Identification of reference genes for real-time quantitative polymerase chain reaction based gene expression studies on various Olive (Olea europaea L.) tissues

2018 ◽  
Vol 93 (6) ◽  
pp. 644-651 ◽  
Author(s):  
Kaan Hürkan ◽  
Fatih Sezer ◽  
Aslıhan Özbilen ◽  
Kemal M. Taşkın
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Reference Genes ◽  
Quantitative Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Olea Europaea L ◽  
Expression Studies ◽  
Polymerase Chain ◽  
Gene Expression Studies

scholarly journals Validation of the Reference Genes for the Gene Expression Studies in Different Cell Lines of Pig

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Chun-Di Xie ◽  
Bingyuan Wang ◽  
Zhao-Ji Shen ◽  
Wen-Ye Yao ◽  
Hong Ao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Cell Lines ◽  
Reference Genes ◽  
Theoretical Basis ◽  
Target Genes ◽  
Chain Reaction ◽  
Expression Studies ◽  
Polymerase Chain ◽  
Gene Expression Studies

Reverse transcription quantitative real-time polymerase chain reaction is one of the important methods to investigate gene expression in cells and tissues. However, if the data cannot be normalized with appropriate reference genes, the results may be unreliable. In this study, we detected the expression of 15 reference genes in three pig cell lines. The results showed that SDHA and ALDOA were the most stable reference genes in 3D4/21 cells. TOP2B, TBP, and PPIA were the most stable reference genes in PK-15 cells. SDHA and ALDOA were the most stable reference genes in IPEC-J2 cells. In addition, each cell line only needs to use two reference genes to standardize the expression of target genes. Taken together, this study provides a reference for different pig cell lines to select reference genes and also provides a theoretical basis for the use of these cell lines in related functional researches.

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