Identification of suitable reference genes for gene expression studies of human serous ovarian cancer by real-time polymerase chain reaction

2009 ◽  
Vol 394 (1) ◽  
pp. 110-116 ◽  
Author(s):  
Yan-Li Li ◽  
Feng Ye ◽  
Ying Hu ◽  
Wei-Guo Lu ◽  
Xing Xie
Keyword(s):  
Gene Expression ◽  
Ovarian Cancer ◽  
Polymerase Chain Reaction ◽  
Reference Genes ◽  
Serous Ovarian Cancer ◽  
Chain Reaction ◽  
Expression Studies ◽  
Polymerase Chain ◽  
Gene Expression Studies ◽  
Suitable Reference

scholarly journals Identification and Validation of Reference Genes for qRT-PCR Studies of Gene Expression inDioscorea opposita

2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
Xiting Zhao ◽  
Xiaoli Zhang ◽  
Xiaobo Guo ◽  
Shujie Li ◽  
Linlin Han ◽  
...  
Keyword(s):  
Gene Expression ◽  
Reference Genes ◽  
Developmental Stages ◽  
Chain Reaction ◽  
Qrt Pcr ◽  
Expression Studies ◽  
Tuber Crop ◽  
Polymerase Chain ◽  
Gene Expression Studies ◽  
Suitable Reference

Quantitative real-time polymerase chain reaction (qRT-PCR) is one of the most common methods for gene expression studies. Data normalization based on reference genes is essential for obtaining reliable results for qRT-PCR assays. This study evaluated potential reference genes of Chinese yam (Dioscorea oppositaThunb.), which is an important tuber crop and medicinal plant in East Asia. The expression of ten candidate reference genes across 20 samples from different organs and development stages was assessed. We identified the most stable genes for qRT-PCR studies using combined samples from different organs. Our results also suggest that different suitable reference genes or combinations of reference genes for normalization should be applied according to different organs and developmental stages. To validate the suitability of the reference genes, we evaluated the relative expression ofPE2.1andPE53, which are two genes that may be associated with microtuber formation. Our results provide the foundation for reference gene(s) selection inD. oppositaand will contribute toward more accurate gene analysis studies of the genusDioscorea.

scholarly journals Validation of the Reference Genes for the Gene Expression Studies in Different Cell Lines of Pig

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Chun-Di Xie ◽  
Bingyuan Wang ◽  
Zhao-Ji Shen ◽  
Wen-Ye Yao ◽  
Hong Ao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Cell Lines ◽  
Reference Genes ◽  
Theoretical Basis ◽  
Target Genes ◽  
Chain Reaction ◽  
Expression Studies ◽  
Polymerase Chain ◽  
Gene Expression Studies

Reverse transcription quantitative real-time polymerase chain reaction is one of the important methods to investigate gene expression in cells and tissues. However, if the data cannot be normalized with appropriate reference genes, the results may be unreliable. In this study, we detected the expression of 15 reference genes in three pig cell lines. The results showed that SDHA and ALDOA were the most stable reference genes in 3D4/21 cells. TOP2B, TBP, and PPIA were the most stable reference genes in PK-15 cells. SDHA and ALDOA were the most stable reference genes in IPEC-J2 cells. In addition, each cell line only needs to use two reference genes to standardize the expression of target genes. Taken together, this study provides a reference for different pig cell lines to select reference genes and also provides a theoretical basis for the use of these cell lines in related functional researches.

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