A functional peptide-mediated colorimetric assay for mercury ion based on dual-modified gold nanoparticles

2021 ◽  
pp. 114369
Author(s):  
Xin-Yi Li ◽  
Miao-Miao Zhang ◽  
Xiao-Dong Zhou ◽  
Ji-Ming Hu
Keyword(s):  
Gold Nanoparticles ◽  
Colorimetric Assay ◽  
Mercury Ion ◽  
Functional Peptide

An Effort to Making a Colorimitric Nano-Biosensor for Vibrio cholera Detection

2020 ◽  
Vol 16 (5) ◽  
pp. 793-804
Author(s):  
Naimeh Mahheidari ◽  
Jamal Rashidiani ◽  
Hamid Kooshki ◽  
Khadijeh Eskandari
Keyword(s):  
Gold Nanoparticles ◽  
Colorimetric Assay ◽  
Au Nanoparticle ◽  
Bacteria Detection ◽  
Great Promise ◽  
Bacterial Cells ◽  
Vibrio Cholera ◽  
Minimum Concentration ◽  
Vis Spectroscopy ◽  
Fast Procedure

Background: Today, nanoparticles hold great promise in biomedical researches and applications including bacteria detection. The rapid and sensitive outcomes of bacteria detection strategies using nanoparticle conjugates become determinative, especially in bacterial outbreaks. In the current research, we focused on detecting V. cholera bacteria and its toxin using a thiocyanate/Au nanoparticle. Thiocyanate adsorbed strongly on the surface of gold nanoparticles and changed the surface by enhancing surface plasmon resonance of gold nanoparticles. Objective: This method is tried to introduce a simple and fast procedure to assay vibrio cholera. So, it is observed by the naked eyes as well. Methods: We used two antibodies (Ab) for V. cholera detection: a) a primary antibody conjugated to magnetic nanoparticles (MNPs) for trapping V. cholera bacterial cells, and b) a secondary Abconjugated thiocyanate-GNPs as a colorimetric detector. Then, an immuno-magnetic separation system connected to a colorimetric assay was designed based on the GNPs. The results were measured by ultraviolet-visible (UV-Vis) spectroscopy. Results: The results showed that gold nanoparticles are an appropriate optical assay for detecting biological samples in a minimum concentration and also it can be easily seen by the naked eyes. The linear range of this biosensor is 3.2×104 to 28×104 cells per ml. Conclusion: In this research, a colorimetric immune assay based on gold nanoparticles was designed to improve the sensitivity of V. cholera detection. Also, this method can be used for the detection of other biological agents.

scholarly journals A dual colorimetric probe for rapid environmental monitoring of Hg2+ and As3+ using gold nanoparticles functionalized with d-penicillamine

RSC Advances ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 5456-5465
Author(s):  
Su-Jin Yoon ◽  
Yun-Sik Nam ◽  
Yeonhee Lee ◽  
In Hwan Oh ◽  
Kang-Bong Lee
Keyword(s):  
Gold Nanoparticles ◽  
Environmental Monitoring ◽  
Colorimetric Assay ◽  
Colorimetric Probe ◽  
Highly Sensitive ◽  
Dual Detection

A highly sensitive and selective colorimetric assay for the dual detection of Hg2+ and As3+ using gold nanoparticles (AuNPs) conjugated with d-penicillamine (DPL) was developed.

scholarly journals Enhancement of the Peroxidase-Like Activity of Iodine-Capped Gold Nanoparticles for the Colorimetric Detection of Biothiols

Biosensors ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 113 ◽  
Author(s):  
Chia-Chen Chang ◽  
Tsz-Lian Hsu ◽  
Chie-Pein Chen ◽  
Chen-Yu Chen
Keyword(s):  
Gold Nanoparticles ◽  
Catalytic Activity ◽  
Detection Limit ◽  
Enzymatic Activity ◽  
Catalytic Properties ◽  
Colorimetric Assay ◽  
Colorimetric Detection ◽  
Synergetic Effect ◽  
Vis Spectroscopy ◽  
Wide Range

A colorimetric assay was developed for the detection of biothiols, based on the peroxidase-like activity of iodine-capped gold nanoparticles (AuNPs). These AuNPs show a synergetic effect in the form of peroxidase-mimicking activity at the interface of AuNPs, while free AuNPs and iodine alone have weak catalytic properties. Thus, iodine-capped AuNPs possess good intrinsic enzymatic activity and trigger the oxidation of 3,3’,5,5’-tetramethylbenzidine (TMB), leading to a change in color from colorless to yellow. When added to solution, biothiols, such as cysteine, strongly bind to the interface of AuNPs via gold-thiol bonds, inhibiting the catalytic activity of AuNPs, resulting in a decrease in oxidized TMB. Using this strategy, cysteine could be linearly determined, at a wide range of concentrations (0.5 to 20 μM), with a detection limit of 0.5 μM using UV-Vis spectroscopy. This method was applied for the detection of cysteine in diluted human urine.

scholarly journals Development of Label-Free Colorimetric Assay for MERS-CoV Using Gold Nanoparticles

ACS Sensors ◽  
2019 ◽  
Vol 4 (5) ◽  
pp. 1306-1312 ◽  
Author(s):  
Hanbi Kim ◽  
Minseon Park ◽  
Joonki Hwang ◽  
Jin Hwa Kim ◽  
Doo-Ryeon Chung ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Colorimetric Assay ◽  
Label Free

Paper-based device for the colorimetric assay of bilirubin based on in-situ formation of gold nanoparticles

2019 ◽  
Vol 187 (1) ◽  
Author(s):  
Resmi P. Edachana ◽  
Abishek Kumaresan ◽  
Vidhya Balasubramanian ◽  
Ramachandran Thiagarajan ◽  
Bipin G. Nair ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Colorimetric Assay ◽  
In Situ Formation

Colorimetric Detection of Trace Arsenic(III) in Aqueous Solution Using Arsenic Aptamer and Gold Nanoparticles

2014 ◽  
Vol 67 (5) ◽  
pp. 813 ◽  
Author(s):  
Minglei Yu
Keyword(s):  
Aqueous Solution ◽  
Gold Nanoparticles ◽  
Detection Limit ◽  
Colorimetric Assay ◽  
Colorimetric Detection ◽  
Colour Change ◽  
Colorimetric Sensing ◽  
Detection Range ◽  
Colorimetric Indicator ◽  
Blue Solution

In this study, trace arsenic(iii) (AsIII) in aqueous solution was detected by applying a classical aptamer-based gold nanoparticles colorimetric sensing strategy. An arsenic aptamer was used as a sensing probe and gold nanoparticles as a colorimetric indicator. In the absence of AsIII, the gold nanoparticles were stabilised by the arsenic aptamer and remained dispersed at high NaCl concentrations, displaying a red solution. Contrarily, in the presence of AsIII, the gold nanoparticles were prone to aggregation, owing to the formation of aptamer–AsIII complex between the arsenic aptamer and AsIII, and thus exhibited a blue solution. By monitoring the colour change, a simple and fast colorimetric assay for AsIII was established with a detection range of 1.26–200 ppb and a detection limit of 1.26 ppb. Because this colorimetric assay only involves common reagents and can be assessed visually, it holds great potential for arsenic(iii) monitoring in environment-related and other applications.

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