Gold nanoparticles-based colorimetric assay for rapid detection of Salmonella species in food samples

Background: Today, nanoparticles hold great promise in biomedical researches and applications including bacteria detection. The rapid and sensitive outcomes of bacteria detection strategies using nanoparticle conjugates become determinative, especially in bacterial outbreaks. In the current research, we focused on detecting V. cholera bacteria and its toxin using a thiocyanate/Au nanoparticle. Thiocyanate adsorbed strongly on the surface of gold nanoparticles and changed the surface by enhancing surface plasmon resonance of gold nanoparticles. Objective: This method is tried to introduce a simple and fast procedure to assay vibrio cholera. So, it is observed by the naked eyes as well. Methods: We used two antibodies (Ab) for V. cholera detection: a) a primary antibody conjugated to magnetic nanoparticles (MNPs) for trapping V. cholera bacterial cells, and b) a secondary Abconjugated thiocyanate-GNPs as a colorimetric detector. Then, an immuno-magnetic separation system connected to a colorimetric assay was designed based on the GNPs. The results were measured by ultraviolet-visible (UV-Vis) spectroscopy. Results: The results showed that gold nanoparticles are an appropriate optical assay for detecting biological samples in a minimum concentration and also it can be easily seen by the naked eyes. The linear range of this biosensor is 3.2×104 to 28×104 cells per ml. Conclusion: In this research, a colorimetric immune assay based on gold nanoparticles was designed to improve the sensitivity of V. cholera detection. Also, this method can be used for the detection of other biological agents.

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A dual colorimetric probe for rapid environmental monitoring of Hg2+ and As3+ using gold nanoparticles functionalized with d-penicillamine

RSC Advances ◽

10.1039/d0ra08525a ◽

2021 ◽

Vol 11 (10) ◽

pp. 5456-5465

Author(s):

Su-Jin Yoon ◽

Yun-Sik Nam ◽

Yeonhee Lee ◽

In Hwan Oh ◽

Kang-Bong Lee

Keyword(s):

Gold Nanoparticles ◽

Environmental Monitoring ◽

Colorimetric Assay ◽

Colorimetric Probe ◽

Highly Sensitive ◽

Dual Detection

A highly sensitive and selective colorimetric assay for the dual detection of Hg2+ and As3+ using gold nanoparticles (AuNPs) conjugated with d-penicillamine (DPL) was developed.

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Immunochromatographic Assay Based on Polydopamine-Decorated Iridium Oxide Nanoparticles for the Rapid Detection of Salbutamol in Food Samples

ACS Applied Materials & Interfaces ◽

10.1021/acsami.1c06724 ◽

2021 ◽

Author(s):

Shuang Zhao ◽

Tong Bu ◽

Kairong Yang ◽

Zhihao Xu ◽

Feier Bai ◽

...

Keyword(s):

Rapid Detection ◽

Food Samples ◽

Iridium Oxide ◽

Immunochromatographic Assay ◽

Oxide Nanoparticles

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A BIENZYME ELECTROCHEMICAL BIOSENSOR COUPLED WITH IMMUNOMAGNETIC SEPARATION FOR RAPID DETECTION OF ESCHERICHIA COLI O157:H7 IN FOOD SAMPLES

Transactions of the ASAE ◽

10.13031/2013.7853 ◽

2002 ◽

Vol 45 (1) ◽

Cited By ~ 31

Author(s):

C. Ruan ◽

H. Wang ◽

Y. Li

Keyword(s):

Escherichia Coli ◽

Rapid Detection ◽

Electrochemical Biosensor ◽

Immunomagnetic Separation ◽

Food Samples ◽

Escherichia Coli O157

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Application of nanomaterials in microbial-cell biosensor constructions

Chemical Papers ◽

10.2478/s11696-014-0602-2 ◽

2015 ◽

Vol 69 (1) ◽

Cited By ~ 9

Author(s):

Jana Šefčovičová ◽

Jan Tkac

Keyword(s):

Carbon Nanotubes ◽

Gold Nanoparticles ◽

Rapid Detection ◽

Food Industry ◽

Low Cost ◽

Microbial Cell ◽

Direct Connection ◽

Qualitative Detection ◽

Analytical Tools ◽

Cell Biosensor

AbstractMicrobial cell biosensors, where cells are in direct connection with a transducer enabling quantitative and qualitative detection of an analyte, are very promising analytical tools applied mainly for assays in the environmental field, food industry or biomedicine. Microbial cell biosensors are an excellent alternative to conventional analytical methods due to their specificity, rapid detection and low cost of analysis. Nowadays, nanomaterials are often used in the construction of biosensors to improve their sensitivity and stability. In this review, the combination of microbial and other individual cells with different nanomaterials (carbon nanotubes, graphene, gold nanoparticles, etc.) for the construction of biosensors is described and their applications are provided as well.

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