WITHDRAWN: Investigation on the spectrum-effect relationships of ethyl acetate extract from Radix Isatidis based on High Performance Liquid Chromatography and microcalorimetry

2008 ◽  
Author(s):  
Weijun Kong ◽  
Yanling Zhao ◽  
Xiaohe Xiao ◽  
Limei Shan ◽  
Weiying Guo
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Ethyl Acetate ◽  
High Performance ◽  
Ethyl Acetate Extract ◽  
Radix Isatidis ◽  
Spectrum Effect

scholarly journals DETERMINATION OF ANNONACYN COMPOUND BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY ON THE EXTRACT OF Annona muricata LINN SEED FOR PESTICIDE FORMULA

2010 ◽  
Vol 5 (3) ◽  
pp. 215-218 ◽  
Author(s):  
Freddy L Wurangian
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Ethyl Acetate ◽  
Mobile Phase ◽  
High Performance ◽  
Ethyl Acetate Extract ◽  
Ethanolic Extract ◽  
Raw Material ◽  
Limit Detection

Determination of annonacyn grade, a main insecticide compound has been done in the ethanolic and ethyl acetate extracts produced by soxhletation, and ethyl acetate extract produced by fractionation of ethanolic extract to find out the most active extract to be used as raw material of pesticide formulae. Analysis method used the reverse phase high performance liquid chromatography with column of Novapack ODS C-18 (waters; 3.9 x 150 nm), mobile phase was the mixture of acetonitrile-water. The result of this research showed the optimum condition as follows: the mobile phase was acetonitrile-water (60:40), flow rate of 0.2 mL min-1, injection volume of 5.0 L, detector UV, wavelength () at 220 nm with AUFS of 1.00. The limit detection of annonacyn was 0.01 g, annonacyn grade in ethanolic extract produced by soxhletation: 0.0405 + 0.0021%, in ethyl acetate extract produced by soxhletation: 0.0293 + 0.0009%, in ethyl acetate extract produced by fractionation of ethanolic extract: 0.1003 + 0.0018%. The precision and accuracy of annonacyn in this research were respectively obtained 8.89% - 1.92%, and 4.91% - 7.28%, at the concentration of 5.00 g mL-1 - 25.00 g mL-1. The sensitivity was 0.75

scholarly journals Phytochemical analysis and antioxidant capacity ofTabernaemontana catharinensis A. DC. Fruits and branches

2014 ◽  
Vol 86 (2) ◽  
pp. 881-888 ◽  
Author(s):  
MARIANA PIANA ◽  
ALINE A. BOLIGON ◽  
THIELE F. DE BRUM ◽  
MARINA ZADRA ◽  
BIANCA V. BELKE ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Ethyl Acetate ◽  
Antioxidant Capacity ◽  
Chlorogenic Acid ◽  
Condensed Tannins ◽  
High Performance ◽  
Ethyl Acetate Fraction ◽  
Phytochemical Analysis ◽  
Dpph Method

The antioxidant capacity of the crude extract and fractions ofTabernaemontana catharinensis fruits and branches, was evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and the content of polyphenols, flavonoids, alkaloids and condensed tannins were determined by the spectrophotometric method. The ethyl acetate fraction of the fruits and the n-butanol fraction of the branches showed IC50 of 181.82 µg/mL and 78.19 µg/mL, respectively. All fractions were analyzed by high performance liquid chromatography (HPLC), in the branches were quantified chlorogenic acid in the chloroform (8.96 mg/g), ethyl acetate (4.31 mg/g) and n-butanol (3.33 mg/g) fractions; caffeic acid in the ethyl acetate (5.24 mg/g) and n-butanol (1.81 mg/g); gallic acid (0.52 mg/g) in the n-butanol. In the fruits, chlorogenic acid in the chloroform (1.67 mg/g); rutin in the ethyl acetate (3.45 mg/g) and n-butanol (8.98 mg/g) fractions. The present study showed that these quantified compounds can contribute to antioxidant capacity which was higher in the branches than in the fruits.

Chromatographic behaviour of some porphyrins and their complexes with Zn, Pd(II) and Cu(II)

2000 ◽  
Vol 04 (02) ◽  
pp. 209-214 ◽  
Author(s):  
M. I. UVAROVA ◽  
G. D. BRYKINA ◽  
O. A. SHPIGUN
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Ethyl Acetate ◽  
Mobile Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Zinc Complexes ◽  
Acetate Concentration ◽  
Hydrophobic Compounds ◽  
Retention Parameters

In this work the influence of the porphyrin structure and of the nature of the mobile phase upon retention parameters is examined by means of reversed phase high-performance liquid chromatography (HPLC). Acetonitrile–ethyl acetate and other mixtures were used as eluents. An increase in the retention of azo- and benzosubstituted porphyrins as well as of those containing a large number of carbon atoms as substituents of macrocycles may be noted. A variation in the polarity of the mobile phase affects the retention of the ligands more than that of their zinc complexes. The retention of the most hydrophobic compounds may be well described in coordinates lg k'– lg M. For less hydrophobic porphyrins these dependences are close to linear only within limited intervals of mobile phase ethyl acetate concentration. The best separation of zinc complexes was achieved with acetonitrile as the eluent. The detection limit of porphyrin ligands and complexes with metals is n × 10-8 M.

Development and Validation of a High-Performance Liquid Chromatography Method for Standardization of the Bioactive Ethyl Acetate Fraction of Alstonia scholaris (Linn.) R. Br. Growing in Egypt

2013 ◽  
Vol 68 (9-10) ◽  
pp. 376-383
Author(s):  
Hesham I. El-Askary ◽  
Mahmoud M. El-Olemy ◽  
Maha M. Salama ◽  
Mahetab H. Amer
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Ethyl Acetate ◽  
High Performance ◽  
Ethanolic Extract ◽  
Ethyl Acetate Fraction ◽  
Hplc Method ◽  
Spectroscopic Techniques ◽  
Alstonia Scholaris ◽  
Relative Standard

Bio-guided fractionation of the ethanolic extract of the leaves of Alstonia scholaris (Apocynaceae) growing in Egypt was carried out to evaluate its antihyperglycemic acti vity in alloxan-induced diabetic rats and its hepatoprotective activity against CCl4-induced hepatotoxicity in rats. The ethyl acetate fraction of the ethanolic extract showed the highest antihyperglycemic [(133.6 ± 4.2) mg/mL, relative to metformin with (92.3 ± 2.7) mg/mL] and hepatoprotective [(37.9 ± 1.4) U/L, relative to silymarin with (29.7 ± 0.8) U/L] activities. Four compounds were isolated from this fraction, and identifi ed by spectroscopic techniques and by comparison with reported data: caffeic acid and isoquercitrin for the fi rst time from this plant, in addition to quercetin 3-O-β-D-xylopyranosyl (1''' →2")-β-D-galactopyranoside (major compound) and chlorogenic acid. A validated reversed phase-high-performance liquid chromatography (RP-HPLC) method was developed for the standardization of the bio active ethyl acetate fraction. The calibration curve showed good linearity (r2 > 0.999) within tested ranges. The relative standard deviation of the method was less than 3% for intra- (0.4 - 2.0%) and inter-day (1.9 - 2.8%) assays. Mean recovery of the method was within the range of 98.5 - 102.5%. The minimum detectable concentration of the analyte (LOD) was found to be 0.04 μg/mL. This developed HPLC method was shown to be simple, rapid, precise, reproducible, robust, specifi c, and accurate for quality assessment of the bioactive fraction

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