Spectrum–effect relationships between high-performance liquid chromatography fingerprints and anti-inflammatory activities of Leontopodium leontopodioides (Willd.) Beauv.

2019 ◽  
Vol 1104 ◽  
pp. 11-17 ◽  
Author(s):  
Yue Zhao ◽  
Xian-Min You ◽  
Hong Jiang ◽  
Gui-Xin Zou ◽  
Bing Wang
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Anti Inflammatory ◽  
Spectrum Effect

Chemical Profiling of Embelia ribes by Ultra-High-Performance Liquid Chromatography Quadrupole Time-of-Flight Tandem Mass Spectrometry and Its Antioxidant and Anti-inflammatory Activities In Vitro

2020 ◽  
Vol 58 (3) ◽  
pp. 241-250
Author(s):  
Sa Guo ◽  
Mingzhen He ◽  
Mi Liu ◽  
Wengping Huang ◽  
Hui Ouyang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
High Performance ◽  
Time Of Flight ◽  
Tandem Mass ◽  
Ether Extract ◽  
Embelia Ribes ◽  
Anti Inflammatory

Abstract Embelia ribes is a traditional Chinese medicine compound used as a remedy for various diseases. Nevertheless, detailed information regarding its chemical composition is unavailable. Herein, ultra-high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry was used to characterize the components of E. ribes. A total of 56 compounds, including 16 phenolics, 16 flavonoids, 4 coumarins, 5 fatty acids and 15 other compounds were identified. Furthermore, the total phenolic and total flavonoid content was also assessed; the acetic ether extract of E. ribes was an ideal source of phenolics (308.16 ± 0.00 mg gallic acid equivalents/g of extract) and flavonoids (62.00 ± 0.01 mg rutin equivalents/g of extract). Additionally, acetic ether extract exhibited a high antioxidation effect (ferric reducing activity power: 0.15 ± 0.01 mg/mL; 1,1-diphenyl-2-picrylhydrazyl: 0.18 ± 0.01 mg/mL; 2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid: 0.06 ± 0.01 mg/mL). Further, the nitric oxide concentration in lipopolysaccharide-simulated macrophage RAW 264.7 cells and the pro-inflammatory cytokines (TNF-α and IL-6) were suppressed by acetic ether extract. These findings support the notion that E. ribes is an ideal antioxidant and anti-inflammatory agent.

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