Intramembranous bone tissue response to biodegradable octacalcium phosphate implant

We have previously shown that synthetic octacalcium phosphate (OCP) displays highly osteoconductive and biodegradable characteristics. However, OCP cannot be sintered without thermal decomposition due to the existence of water molecules in the structure. The acquisition of the moldability and the improvement of the handling property in this material are subjects for the clinical use. In the present study, we prepared OCP complex with hyaluronic acid (Hya) that could be used in the injectable form and further examined the bone tissue reaction to cortical bone by placing the complex directly on an 8-weeks-old ICR mouse calvaria in comparison with the placement of OCP granules only. The granule form of OCP (between 300 to 500 μm in diameter) was mixed with sodium hyaluronic acid with molecular weights 90 x 104. The complex revealed an injectable characteristic if it was utilized in a syringe. After polytetrafluoroethylen ring was mounted on mouse calvaria, the inner space of the ring was filled with the complex and left the complex as it is for 6 weeks. Histological examination using the decalcified specimens indicated that the OCP/Hya complex exhibited greater bone formation than OCP granules only group within the ring at 6 weeks. The results suggested that the OCP/Hya complex could be used as an injectable and osteoconductive bone substitute material in many clinical situations.

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Osteoblast and bone tissue response to surface modified zirconia and titanium implant materials

Dental Materials ◽

10.1016/j.dental.2013.04.003 ◽

2013 ◽

Vol 29 (7) ◽

pp. 763-776 ◽

Cited By ~ 52

Author(s):

Ralf J. Kohal ◽

Maria Bächle ◽

Wael Att ◽

Saad Chaar ◽

Brigitte Altmann ◽

...

Keyword(s):

Bone Tissue ◽

Titanium Implant ◽

Tissue Response ◽

Implant Materials ◽

Surface Modified

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Bone tissue response to four-month antiorthostatic bedrest: A bone histomorphometric study

Calcified Tissue International ◽

10.1007/bf00334546 ◽

1992 ◽

Vol 51 (3) ◽

pp. 189-194 ◽

Cited By ~ 54

Author(s):

Sabinne Palle ◽

Laurence Vico ◽

Sandrine Bourrin ◽

Christian Alexandre

Keyword(s):

Bone Tissue ◽

Tissue Response ◽

Histomorphometric Study

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Evaluation of Bone Ingrowth in Free Form Fabricated Scaffolds

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.361-363.919 ◽

2007 ◽

Vol 361-363 ◽

pp. 919-922

Author(s):

Erik Adolfsson ◽

Johan Malmström ◽

Peter Thomsen

Keyword(s):

Bone Tissue ◽

Sintered Density ◽

Bone Ingrowth ◽

Casting Process ◽

Tissue Response ◽

Free Form ◽

Bone Area ◽

Colloidal Processing ◽

Sintering Condition ◽

Rabbit Femur

Colloidal processing was used to cast zirconia and hydroxyapatite materials. The cast materials reached densities around 99% when sintered at 1500°C and 1200°C respectively. By controlling the colloidal process the sintered density of hydroxyapatite was also reduced to around 80% when the same sintering condition was used. The casting process was combined with free form fabrication to prepare designed scaffolds with identical macroporosity. These scaffolds were used to evaluate the early bone tissue response in rabbit femur. After six weeks of implantation the bone area in scaffolds of zirconia and hydroxyapatite were compared. In scaffolds of hydroxyapatite the bone area was roughly three times larger compared to corresponding scaffolds of zirconia. When the scaffolds of hydroxyapatite also contained an open microporosity of around 20% the amount of bone was even more pronounced. The results showed the importance of the material composition and the microstructure on the bone regenerating performance of scaffolds.

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Bone tissue response to zinc polycarboxylate cement and zincfree amalgam

Journal of Endodontics ◽

10.1016/s0099-2399(76)80134-3 ◽

1976 ◽

Vol 2 (7) ◽

pp. 203-208 ◽

Cited By ~ 9

Author(s):

R ZARTNER ◽

G JAMES ◽

B BURCH

Keyword(s):

Bone Tissue ◽

Tissue Response

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Bone Tissue Response to Different Grown Crystal Batches of Octacalcium Phosphate in Rat Long Bone Intramedullary Canal Area

International Journal of Molecular Sciences ◽

10.3390/ijms22189770 ◽

2021 ◽

Vol 22 (18) ◽

pp. 9770

Author(s):

Yukari Shiwaku ◽

Ryo Hamai ◽

Shinichi Sato ◽

Susumu Sakai ◽

Kaori Tsuchiya ◽

...

Keyword(s):

Octacalcium Phosphate ◽

Tissue Response ◽

Ion Concentration ◽

Long Bone ◽

Dissolution Behavior ◽

Control Group ◽

Tubular Bone ◽

Biological Responses ◽

Intramedullary Canal ◽

Rat Tibia

The microstructure of biomaterials influences the cellular and biological responses in the bone. Octacalcium phosphate (OCP) exhibits higher biodegradability and osteoconductivity than hydroxyapatite (HA) during the conversion process from OCP to HA. However, the effect of the microstructure of OCP crystals on long tubular bones has not been clarified. In this study, two types of OCPs with different microstructures, fine-OCP (F-OCP) and coarse-OCP (C-OCP), were implanted in rat tibia for 4 weeks. F-OCP promoted cortical bone regeneration compared with C-OCP. The osteoclasts appearance was significantly higher in the C-OCP group than in the control group (defect only) at 1-week post-implantation. To investigate whether the solubility equilibrium depends on the different particle sizes of OCPs, Nano-OCP, which consisted of nanometer-sized OCPs, was prepared. The degree of supersaturation (DS) tended to decrease modestly in the order of C-OCP, F-OCP, and Nano-OCP with respect to HA and OCP in Tris-HCl buffer. F-OCP showed a higher phosphate ion concentration and lower calcium ion concentration after immersion in the buffer than C-OCP. The crystal structures of both OCPs tended to be converted to HA by rat abdominal implantation. These results suggest that differences in the microstructure of OCPs may affect osteoclastogenesis and result in osteoconductivity of this material in long tubular bone by altering dissolution behavior.

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Bone tissue response to implant surfaces functionalized with phosphate-containing polymers

Clinical Oral Implants Research ◽

10.1111/clr.12053 ◽

2012 ◽

Vol 25 (1) ◽

pp. 91-100 ◽

Cited By ~ 5

Author(s):

Marcio Vivan Cardoso ◽

Amol Chaudhari ◽

Yasuhiro Yoshida ◽

Bart Van Meerbeek ◽

Ignace Naert ◽

...

Keyword(s):

Bone Tissue ◽

Tissue Response

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Human non-decalcified histology of three dental implants 45 months under function—a case report

International Journal of Implant Dentistry ◽

10.1186/s40729-019-0184-4 ◽

2019 ◽

Vol 5 (1) ◽

Author(s):

Rafael Silveira Faeda ◽

Suzana Clesia Silverio do Nascimento ◽

Pâmela Leticia Santos ◽

Rodolfo Jorge Boeck ◽

Rafael Sartori ◽

...

Keyword(s):

Case Report ◽

Bone Tissue ◽

Rare Event ◽

Polarized Light ◽

Collagen Fibers ◽

Titanium Implants ◽

Tissue Response ◽

Internal Diameter ◽

Human Beings ◽

Circularly Polarized Light

Abstract Background Fracture of an implant is a quite rare event but represents an important opportunity to evaluate the peri-implant bone tissue response to implant overload in human beings. This study aimed to evaluate bone tissue around three fractured titanium implants retrieved from a human maxilla, by histomorphometric and birefringence analyses. Case report For this, the implants and the surrounding bone were removed after having been united to a tooth in function for 45 months, by a 4-mm internal diameter trephine bur, following an undecalcified section was obtained. The results showed a rate of 77.3% of bone-to-implant contact (BIC) and 80.3% of bone area filling within the limits of the implant threads. Under circularly polarized light microscopy investigation, the amount of the transverse collagen fibers was of 48.11%, and the amount of the longitudinal collagen fibers was of 51.89%. Conclusion Within the limitation of this study, the possible cause of the implant fracture could be the association of overload, inadequate implant diameter, and fragile internal hexagon connection.

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