scholarly journals Bone Tissue Response to Different Grown Crystal Batches of Octacalcium Phosphate in Rat Long Bone Intramedullary Canal Area

2021 ◽  
Vol 22 (18) ◽  
pp. 9770
Author(s):  
Yukari Shiwaku ◽  
Ryo Hamai ◽  
Shinichi Sato ◽  
Susumu Sakai ◽  
Kaori Tsuchiya ◽  
...  
Keyword(s):  
Octacalcium Phosphate ◽  
Tissue Response ◽  
Ion Concentration ◽  
Long Bone ◽  
Dissolution Behavior ◽  
Control Group ◽  
Tubular Bone ◽  
Biological Responses ◽  
Intramedullary Canal ◽  
Rat Tibia

The microstructure of biomaterials influences the cellular and biological responses in the bone. Octacalcium phosphate (OCP) exhibits higher biodegradability and osteoconductivity than hydroxyapatite (HA) during the conversion process from OCP to HA. However, the effect of the microstructure of OCP crystals on long tubular bones has not been clarified. In this study, two types of OCPs with different microstructures, fine-OCP (F-OCP) and coarse-OCP (C-OCP), were implanted in rat tibia for 4 weeks. F-OCP promoted cortical bone regeneration compared with C-OCP. The osteoclasts appearance was significantly higher in the C-OCP group than in the control group (defect only) at 1-week post-implantation. To investigate whether the solubility equilibrium depends on the different particle sizes of OCPs, Nano-OCP, which consisted of nanometer-sized OCPs, was prepared. The degree of supersaturation (DS) tended to decrease modestly in the order of C-OCP, F-OCP, and Nano-OCP with respect to HA and OCP in Tris-HCl buffer. F-OCP showed a higher phosphate ion concentration and lower calcium ion concentration after immersion in the buffer than C-OCP. The crystal structures of both OCPs tended to be converted to HA by rat abdominal implantation. These results suggest that differences in the microstructure of OCPs may affect osteoclastogenesis and result in osteoconductivity of this material in long tubular bone by altering dissolution behavior.

Effect of Octacalcium Phosphate-Based Bone Substitute Materials on Oriented Bone Regeneration

2017 ◽  
Vol 758 ◽  
pp. 223-227
Author(s):  
Osamu Suzuki ◽  
Takahisa Anada
Keyword(s):  
Bone Matrix ◽  
Octacalcium Phosphate ◽  
Tissue Response ◽  
Gelatin Sponge ◽  
Natural Polymers ◽  
Preparation Conditions ◽  
Biological Responses ◽  
Bone Substitute Materials ◽  
Collagen Molecules ◽  
Substitute Materials

The characteristics and the biological responses of octacalcium phosphate (OCP) crystals, obtained in the presence of natural polymers, were summarized based on our studies reported. OCP obtained with collagen molecules in the solution had a plate-like morphology while OCP obtained with gelatin molecules in the solution exhibited elongated morphology toward long axis of the crystals. Oriented bone matrix formation was observed by the OCP inclusion in gelatin sponge in a critical-sized rat calvaria defect within the implantation periods around 8 weeks. It seems likely that specific crystal property of OCP obtained in distinct preparation conditions may affect bone tissue response probably through the modulation of OCP crystal characteristics.

scholarly journals Silica/OCP Affects the Viability of Osteoblasts through ROS-Induced Autophagy

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Jianghao Gong ◽  
Shangjun Fu ◽  
Zhenghao Zhou
Keyword(s):  
Flow Cytometry ◽  
Cell Viability ◽  
Protein Level ◽  
Reactive Oxygen ◽  
Octacalcium Phosphate ◽  
Control Group ◽  
Substrate Protein ◽  
Autophagy Inhibitor ◽  
Osteoblast Cell Line

Objective. To explore the effects of silicone gel nanoparticles modified with octacalcium phosphate on the surface (silica/OCP) polymer drugs on the proliferation of osteoblasts and autophagy. Method. Silica/OCP was prepared in vitro, and the quality of the sample preparation was tested through characterization experiments. The osteoblast cell line (hFOB1.19) was treated with silica/OCP, autophagy inhibitor (3-methyladenine (3-MA)), and silica/OCP+3-MA, respectively. The proliferation of hFOB1.19 cells was detected through the methylthiazolyldiphenyl-tetrazolium bromide (MTT) kit. Flow cytometry was used to detect the cell apoptosis. The change in protein beclin1 and P62 expression in hFOB1.19 cells was observed in Western blot. An ROS detection kit was used to detect the content of reactive oxygen species in hFOB1.19 cells. Results. Silica/OCP was a sphere with a particle size of 50 nm to 130 nm and had an OCP phase in electron projection microscopy and X-ray diffraction techniques. The results indicated that OCP successfully modified silica and the material was successfully prepared. An MTT kit and flow cytometry test showed that the cell viability of the cells treated with silica/OCP increased significantly ( P < 0.05 ), and the intracellular apoptosis phenomenon was significantly decreased ( P < 0.05 ) compared to the control group. Moreover, the inhibition of cell viability and promotion of apoptosis caused by the autophagy inhibitor 3-MA can be rescued. Western blotting demonstrated that the protein level of beclin1 in osteoblasts reached the highest after six hours of treatment with silica/OCP, and the protein level of p62, the substrate protein of autophagy, reached the lowest. At the same time, treatment of cells with the autophagy inhibitor 3-MA and silica/OCP+3-MA found that the protein levels of beclin1 and p62 in the silica/OCP+3-MA group were adjusted back compared to the 3-MA group. After adding the autophagy inhibitor, the reactive oxygen content in the cell was significantly increased ( P < 0.05 ) in the silica/OCP group. In the presence of intracellular reactive oxygen inhibitors catalase and silica/OCP, the cell viability of osteoblasts was significantly lower than that of the silica/OCP group but significantly higher than that of the silica/OCP+3-MA group. The apoptosis level of the silica/OCP+catalase group was also significantly lower than that of the silica/OCP+3-MA group ( P < 0.05 ) but was significantly higher than that of the silica/OCP group ( P < 0.05 ). Conclusion. Silica/OCP nanoparticles can upregulate the level of autophagy in osteoblasts and promote the proliferation of osteoblasts.

scholarly journals The effect of mesenchymal stem cells, demineralized bone graft and platelet-rich plasma on osteogenesis in rat tibia defects

2021 ◽  
Vol 11 (Suppl. 1) ◽  
pp. 47-55
Author(s):  
Zozan Erdoğmuş ◽  
Belgin Gülsün
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Graft ◽  
Platelet Rich Plasma ◽  
Female Rats ◽  
Control Group ◽  
Osteoblastic Activity ◽  
Group I ◽  
Rat Tibia ◽  
Demineralized Bone

Aim: Deformities of the jaw and face are often caused by infection, inflammation, and cystic and neoplastic pathological conditions. Defects with various aetiologies should be repaired promptly using the most appropriate approach to reconstruct the anatomical form. To treat defects, bone grafts with various combinations have been used. In particular, combinations including cellular products to enhance osteogenic properties have been implemented. In this study, we aimed to investigate the effects of different materials and cells on bone defects by using mesenchymal stem cells (MSCs), which are thought to have a positive effect on healing, demineralized bone graft (DMB) and platelet-rich plasma (PRP). Methodology: We used 55 female rats weighing between 200-250 g, four of which were used to obtain platelet-rich plasma. The remaining animals were divided into five groups. Group I (n = 6) was the operative control group, Group II (n = 24) was given DMB, Group III (n = 24) was given DMB+PRP, Group IV (n = 24) was given MSC+DBG and Group V (n = 24) was given DMB+PRP+MSC applied to rat tibial defects (10 mm x 3 mm x 2 mm). Results: Statistically significant differences were observed in bone osteoblastic activity in tibia defects among the groups (p<0.05). Conclusion: Bone regeneration was significantly improved in groups where MSCs were used in combination with DMB and PRP.   How to cite this article: Erdoğmuş Z, Gülsün B. The effect of mesenchymal stem cells, demıneralızed bone graft and platelet-rıch plasma on osteogenesıs ın rat tıbıa defects. Int Dent Res 2021;11(Suppl.1):47-55. https://doi.org/10.5577/intdentres.2021.vol11.suppl1.8   Linguistic Revision: The English in this manuscript has been checked by at least two professional editors, both native speakers of English.

scholarly journals Degradation of Phorbol Esters on the Jatropha curcas Linn. Seed by Biological Detoxification

2021 ◽  
Vol 226 ◽  
pp. 00020
Author(s):  
Hany Handajani ◽  
Riza Rahman Hakim ◽  
Ganjar Adhywirawan Sutaro ◽  
Boy Ronald Mavuso ◽  
Zhong–Wen Chang ◽  
...  
Keyword(s):  
Phorbol Ester ◽  
Jatropha Curcas ◽  
Crude Protein ◽  
Phorbol Esters ◽  
Fiber Content ◽  
Crude Fiber ◽  
Control Group ◽  
Crude Fiber Content ◽  
Biological Responses ◽  
Significant Difference

The application of fermentation is one of methods to increase food quality biologically. Availability of Jatropha curcas residual from oil factory be focused as a soybean meal or fish meal replacement. On the other hand, J. curcas residuals possess a toxic compound as well. This study aimed to examine the effect of Aspergillus niger on the nutrition and harmful content of J. curcas as a potential ingredient of feed. In brief, J. curcas residual was fermented with a detoxification method at 3 d, 5 d, and 7 d. Crude protein, fat, and crude fiber content were assessed to discover the biological responses of J. curcas post–fermentation while phorbol ester was evaluated to toxic content post–detoxification. The results showed that crude protein and fat content were highest on 7 d post–fermentation but it was no significant difference (p > 0.05). While crude fiber content showed significant difference which the 3 d fermentation had the highest content of fiber. For phorbol ester content, 3 d fermentation showed a better result than the control group (p < 0.05). The present findings suggest that A. niger is recommendable as starter to reduce fiber and toxic content of J. curcas residual at 3 d fermentation.

Parasitic infection alters the physiological response of a marine gastropod to ocean acidification

Parasitology ◽  
2016 ◽  
Vol 143 (11) ◽  
pp. 1397-1408 ◽  
Author(s):  
C. D. MACLEOD ◽  
R. POULIN
Keyword(s):  
Oxygen Consumption ◽  
Ocean Acidification ◽  
Parasitic Infection ◽  
Marine Organisms ◽  
Ion Concentration ◽  
Metabolic Effects ◽  
Control Group ◽  
Glucose Content ◽  
Hydrogen Ion Concentration ◽  
Consumption Rates

SUMMARYIncreased hydrogen ion concentration and decreased carbonate ion concentration in seawater are the most physiologically relevant consequences of ocean acidification (OA). Changes to either chemical species may increase the metabolic cost of physiological processes in marine organisms, and reduce the energy available for growth, reproduction and survival. Parasitic infection also increases the energetic demands experienced by marine organisms, and may reduce host tolerance to stressors associated with OA. This study assessed the combined metabolic effects of parasitic infection and OA on an intertidal gastropod,Zeacumantus subcarinatus. Oxygen consumption rates and tissue glucose content were recorded in snails infected with one of three trematode parasites, and an uninfected control group, maintained in acidified (7·6 and 7·4 pH) or unmodified (8·1 pH) seawater. Exposure to acidified seawater significantly altered the oxygen consumption rates and tissue glucose content of infected and uninfected snails, and there were clear differences in the magnitude of these changes between snails infected with different species of trematode. These results indicate that the combined effects of OA and parasitic infection significantly alter the energy requirements ofZ. subcarinatus, and that the species of the infecting parasite may play an important role in determining the tolerance of marine gastropods to OA.

Intramembranous bone tissue response to biodegradable octacalcium phosphate implant

2009 ◽  
Vol 5 (5) ◽  
pp. 1756-1766 ◽  
Author(s):  
T. Kikawa ◽  
O. Kashimoto ◽  
H. Imaizumi ◽  
S. Kokubun ◽  
O. Suzuki
Keyword(s):  
Bone Tissue ◽  
Octacalcium Phosphate ◽  
Tissue Response ◽  
Intramembranous Bone

Porous Silk Fibroin Membrane as a Potential Scaffold for Bone Regeneration

2008 ◽  
Vol 396-398 ◽  
pp. 187-190 ◽  
Author(s):  
Raquel Farias Weska ◽  
Grínia M. Nogueira ◽  
Wellington C. Vieira ◽  
Marisa Masumi Beppu
Keyword(s):  
Bone Regeneration ◽  
Silk Fibroin ◽  
Biomedical Application ◽  
Early Stage ◽  
Bone Matrix ◽  
Octacalcium Phosphate ◽  
Ion Concentration ◽  
Phosphate Deposits

The requirements for scaffolds for bone tissue engineering include appropriate chemistry, morphology and structure to promote cell adhesion and synthesis of new bone matrix. Silk fibroin (SF) represents an important biomaterial for biomedical application, due to its suitable mechanical properties, biodegradability, biocompatibility, and versatility in processing. Our group has developed a new method to obtain a porous SF membrane, and the study of its potential for use as a scaffold for bone regeneration was the aim of this study. Porous membranes were obtained from SF solution, through the compression of a material generated by phase separation. For in vitro calcification experiments, porous SF membrane samples were immersed in SBF at pH 7.4 placed in polyethylene flasks. The experiments were carried out for seven days, at 36.5±0.5 °C. After 48 and 96h, the solutions were changed for fresh SBF with the ion concentration 1.5-fold higher than that of the standard one, to accelerate the calcification process. The characterization of morphology and composition of samples was performed by using scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS), respectively. The SEM micrographs indicated that the porous SF membranes presented calcium phosphate deposits after undergoing in vitro calcification. These results were confirmed by EDS spectra, which showed a stoichiometric molar Ca/P ratio ranging from 1.27 to 1.52. This fact may suggest that calcification deposits consisted of mixtures of HAP (Ca/P ratio = 1.67) and transient HAP precursor phases, such as octacalcium phosphate (Ca/P = 1.33) and dicalcium phosphate dehydrate (Ca/P = 1), indicating early stage mineralization. The porous silk fibroin membrane analysed in the current study is a promising material to be used as scaffolds for bone regeneration.

scholarly journals Application of Laser-Induced Bone Therapy by Carbon Dioxide Laser Irradiation in Implant Therapy

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Takahiro Naka ◽  
Satoshi Yokose
Keyword(s):  
Bone Formation ◽  
Laser Irradiation ◽  
Healing Process ◽  
Titanium Implants ◽  
Control Group ◽  
Sham Operation ◽  
Micro Ct ◽  
Rat Tibia ◽  
Implant Therapy ◽  
Laser Group

This study evaluated the application of laser-induced bone therapy (LIBT) to reduce implant healing time in rat tibia. Twenty 10-week-old female Sprague-Dawlay rats were used. The rats received laser irradiation (laser group) or sham operation (control group) on either side of the tibia. Five days after invasion, titanium implants were inserted in proximal tibia. Five, 10, and 20 days after implant placement, tibiae were collected. After taking micro-CT and performing a torque test, the tibiae were decalcified and 8-μm-thick sections were prepared. Specimens were stained with hematoxylin and eosin.Results. Micro-CT images, removal torque values, and histomorphometric analysis data demonstrated a significantly accelerated bone formation in the laser group earlier in the healing process.Conclusion. The use of laser irradiation was effective in promoting bone formation and acquiring osseointegration of titanium implants inserted in rat tibia. LIBT may be suitable for use in implant therapy.

Development of a Bioabsorbable Glass-Reinforced-Glass Intra-Osseous Scaffold for Fracture Healing

2011 ◽  
Vol 9 ◽  
pp. 81-91
Author(s):  
Philip Boughton ◽  
Y. Chen ◽  
C. Thompson ◽  
G. Roger ◽  
Jari Hyvarinen ◽  
...  
Keyword(s):  
Fracture Healing ◽  
Bone Fractures ◽  
Stress Shielding ◽  
Long Bone ◽  
Hematopoietic Tissue ◽  
Shear Tests ◽  
Intramedullary Canal ◽  
Long Bone Fracture ◽  
Torsional Loads ◽  
Mechanically Stabilized

Intramedullary (IM) nails are routinely used to stabilize long bone fractures. They can however lead to stress shielding, pain, migration, obstruct hematopoietic tissue, become a loci for infection, and require subsequent surgical retrieval. Novel intra-osseous scaffold (IOS™) prototypes for fracture healing have been developed to function as a regenerative scaffold to enhance callous formation under mechanically stabilized conditions then resorb. Prototype fixation pins and rod systems were formed from glass-reinforced-glass. Flexion, torsion and shear tests were performed to evaluate the composite pins and rods. A modular rod design was successfully deployed and dilated while in a deformable state. When fitted and gripping the intramedullary canal then set in a rigid state. An obliquely sectioned ovine femur was used as a long bone fracture model for deployment and mechanical verification. Flexural support provided by the intramedullary scaffold was superior to multiple k-wire fixation, while the k-wire approach was more stabilizing under torsional loads. Glass reinforced glass samples were mechanically tested after soaking for up to 4 weeks in saline. Strength and modulus of the composite was reduced to approximately 25% of initial values after 2 weeks.

In Vitro Cytotoxicity and In Vivo Tissue Response Study of Foreign Bodies Iron Based Materials

2015 ◽  
Vol 1112 ◽  
pp. 449-452 ◽  
Author(s):  
Deni Noviana ◽  
Sri Estuningsih ◽  
Devi Paramitha ◽  
Mokhammad Fakhrul Ulum ◽  
Hendra Hermawan
Keyword(s):  
Foreign Body ◽  
Cell Viability ◽  
Direct Method ◽  
The Body ◽  
Tissue Response ◽  
Ion Concentration ◽  
Ion Release ◽  
Iron Based

A foreign body is any object originating outside the body. It may migrate from its entry site and cause pain, inflammation and infection. This study aims to examine in vitro cytotoxicity and in vivo tissue response at different implantation sites of two iron-based foreign body (FeFB) specimens: pure Fe wire, Cr-coated Fe wire, and SS316L wire as control. In vitro cytotoxicity was assessed towards rat smooth muscle cells with direct method of methyl thiazolyl tetrazolium (MTT) assay. In vivo tissue response was examined using mice animal model until day 14 after surgical implantation in subcutaneous nape area and intramuscular right femoral muscle. Cell viability, surface morphology and Fe ion release were examined. Implant density and tissue response were examined by using radiographic imaging and histology, respectively. Results showed that both FeFB specimens exhibited similar cell viability with SS316L. Iron ion concentration was higher in both FeFB medium compared to that of SS316L and with oxide layer formation on their surface. Radiographic analysis showed that the density of both FeFB implants end-side was increased. Meanwhile, histological tissue response at intramuscular sites for FeFB specimens showed a prominent inflammatory response compared to SS316L. Detailed analysis on cell and tissue-material interactions of the iron-based foreign body specimens is discussed further in this article.

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