Evaluating effects of plant growth-promoting rhizobacteria on the radiation use efficiency and yield of soybean (Glycine max) under water deficit stress condition

Agronomic biofortification of horticultural crops using plant growth-promoting rhizobacteria (PGPR) under crop residue incorporation systems remains largely underexploited. Bacillus subtilis (B1), Bacillus laterosporus (B2), or Bacillus amyloliquefaciens (B3) was inoculated on soil containing chili residue, while chili residue without PGPR (NP) served as the control. Two hybrid long cayenne peppers, succeeding a leaf mustard crop were used in the intensive cultivation study. Net photosynthesis, leaf stomatal conductance, transpiration rate, photosynthetic water use efficiency, shoot and root biomass, and fruit yield were evaluated. Derivatives of folate, minerals, and nitrate contents in the pepper fruits were also assessed. B1 elicited higher net photosynthesis and photosynthetic water use efficiency, while B2 and B3 had higher transpiration rates than B1 and NP. B1 and B3 resulted in 27–36% increase in pepper fruit yield compared to other treatments, whereas B3 produced 24–27.5% and 21.9–27.2% higher 5-methyltetrahydrofolate and total folate contents, respectively, compared to B1 and NP. However, chili residue without PGPR inoculation improved fruit calcium, magnesium, and potassium contents than the inoculated treatments. ‘Xin Xian La 8 F1’ cultivar had higher yield and plant biomass, fruit potassium, total soluble solids, and total folate contents compared to ‘La Gao F1.’ Agronomic biofortification through the synergy of Bacillus amyloliquefaciens and chili residue produced better yield and folate contents with a trade-off in the mineral contents of the greenhouse-grown long cayenne pepper.

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Plant Growth-promoting Rhizobacteria and Soybean [Glycine max(L.) Merr.] Growth and Physiology at Suboptimal Root Zone Temperatures

Annals of Botany ◽

10.1006/anbo.1996.0332 ◽

1997 ◽

Vol 79 (3) ◽

pp. 243-249 ◽

Cited By ~ 62

Author(s):

F ZHANG

Keyword(s):

Glycine Max ◽

Plant Growth ◽

Root Zone ◽

Plant Growth Promoting Rhizobacteria ◽

Plant Growth Promoting ◽

Glycine Max L ◽

Growth Promoting

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Pertumbuhan dan Produksi Kedelai Hitam (Glycine max L. Merill) pada Berbagai Jenis Pupuk Kandang dan Konsentrasi Plant Growth Promoting Rhizobacteria

Buletin Anatomi dan Fisiologi ◽

10.14710/baf.5.1.2020.52-59 ◽

2020 ◽

Vol 5 (1) ◽

pp. 52-59

Author(s):

Murtinah Murtinah ◽

Eny Fuskhah ◽

Adriani Darmawati

Keyword(s):

Glycine Max ◽

Plant Growth ◽

Plant Growth Promoting Rhizobacteria ◽

Plant Growth Promoting ◽

Glycine Max L ◽

Growth Promoting ◽

Range Test

Penelitian ini bertujuan untuk mengkaji pengaruh penggunaan jenis pupuk kandang dan berbagai konsentrasi plant growth promoting rhizobacteria (PGPR) terhadap pertumbuhan dan produksi kedelai hitam (Glycine max L. Merill). Rancangan percobaan yang digunakan adalah rancangan acak lengkap pola faktorial. Faktor pertama adalah jenis pupuk kandang (tanpa pupuk, pupuk kandang ayam dan pupuk kandang kambing) dan faktor kedua adalah konsentrasi PGPR (PGPR komersial 5 ml/l air, 0 ml/l air, 5 ml/l air, 12,5 ml/l air, dan 20 ml/l air). Parameter pertumbuhan yang diamati meliputi tinggi tanaman dan jumlah daun. Parameter produksi yang diamati yaitu jumlah polong, berat polong dan bobot 100 biji. Data dianalisis dengan analisis ragam dan dilanjutkan dengan uji jarak berganda Duncan (Duncan’s Multiple Range Test). Hasil penelitian menunjukkan jenis pupuk kandang mampu meningkatkan tinggi tanaman, jumlah daun, jumlah polong, berat polong dan bobot 100 biji. Peningkatan konsentrasi PGPR hanya meningkatkan tinggi tanaman. Tidak menunjukkan adanya interaksi antara jenis pupuk kandang dan konsentrasi PGPR dalam meningkatkan pertumbuhan dan produksi kedelai hitam. Kata kunci : pupuk kandang ayam, pupuk kandang kambing, PGPR, kedelai hitam

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Biofilm Producing Rhizobacteria With Multiple Plant Growth-Promoting Traits Promote Growth of Tomato Under Water-Deficit Stress

Frontiers in Microbiology ◽

10.3389/fmicb.2020.542053 ◽

2020 ◽

Vol 11 ◽

Author(s):

Md. Manjurul Haque ◽

Md Khaled Mosharaf ◽

Moriom Khatun ◽

Md. Amdadul Haque ◽

Md. Sanaullah Biswas ◽

...

Keyword(s):

Plant Growth ◽

Water Deficit ◽

Xanthomonas Campestris ◽

Plant Pathogens ◽

Water Deficit Stress ◽

Rrna Gene ◽

Growth Promoters ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Plant Growth Promoting Traits

Plant growth-promoting rhizobacteria (PGPR) not only enhance plant growth but also control phytopathogens and mitigate abiotic stresses, including water-deficit stress. In this study, 21 (26.9%) rhizobacterial strains isolated from drought-prone ecosystems of Bangladesh were able to form air–liquid (AL) biofilms in the glass test tubes containing salt-optimized broth plus glycerol (SOBG) medium. Based on 16S rRNA gene sequencing, Pseudomonas chlororaphis (ESR3 and ESR15), P. azotoformans ESR4, P. poae ESR6, P. fluorescens (ESR7 and ESR25), P. gessardii ESR9, P. cedrina (ESR12, ESR16, and ESR23), P. veronii (ESR13 and ESR21), P. parafulva ESB18, Stenotrophomonas maltophilia ESR20, Bacillus cereus (ESD3, ESD21, and ESB22), B. horikoshii ESD16, B. aryabhattai ESB6, B. megaterium ESB9, and Staphylococcus saprophyticus ESD8 were identified. Fourier transform infrared spectroscopy studies showed that the biofilm matrices contain proteins, polysaccharides, nucleic acids, and lipids. Congo red binding results indicated that these bacteria produced curli fimbriae and nanocellulose-rich polysaccharides. Expression of nanocellulose was also confirmed by Calcofluor binding assays and scanning electron microscopy. In vitro studies revealed that all these rhizobacterial strains expressed multiple plant growth-promoting traits including N2 fixation, production of indole-3-acetic acid, solubilization of nutrients (P, K, and Zn), and production of ammonia, siderophores, ACC deaminase, catalases, lipases, cellulases, and proteases. Several bacteria were also tolerant to multifarious stresses such as drought, high temperature, extreme pH, and salinity. Among these rhizobacteria, P. cedrina ESR12, P. chlororaphis ESR15, and B. cereus ESD3 impeded the growth of Xanthomonas campestris pv. campestris ATCC 33913, while P. chlororaphis ESR15 and B. cereus ESD21 prevented the progression of Ralstonia solanacearum ATCC® 11696TM. In a pot experiment, tomato plants inoculated with P. azotoformans ESR4, P. poae ESR6, P. gessardii ESR9, P. cedrina ESR12, P. chlororaphis ESR15, S. maltophilia ESR20, P. veronii ESR21, and B. aryabhattai ESB6 exhibited an increased plant growth compared to the non-inoculated plants under water deficit-stressed conditions. Accordingly, the bacterial-treated plants showed a higher antioxidant defense system and a fewer tissue damages than non-inoculated plants under water-limiting conditions. Therefore, biofilm-producing PGPR can be utilized as plant growth promoters, suppressors of plant pathogens, and alleviators of water-deficit stress.

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