Preliminary data on the dietary safety, tolerability and effects on lipid metabolism of the marine microalga Tisochrysis lutea

Single-cell red microalga Porphyridium cruentum is potentially considered to be the bioresource for biofuel and pharmaceutical production. Nitrogen is a kind of nutrient component for photosynthetic P. cruentum. Meanwhile, nitrogen stress could induce to accumulate some substances such as lipid and phycoerythrin and affect its growth and physiology. However, how marine microalga Porphyridium cruentum respond and adapt to nitrogen starvation remains elusive. Here, acclimation of the metabolic reprogramming to changes in the nutrient environment was studied by high-throughput mRNA sequencing in the unicellular red alga P. cruentum. Firstly, to reveal transcriptional regulation, de novo transcriptome was assembled and 8,244 unigenes were annotated based on different database. Secondly, under nitrogen deprivation, 2100 unigenes displayed differential expression (1134 upregulation and 966 downregulation, respectively) and some pathways including carbon/nitrogen metabolism, photosynthesis, and lipid metabolism would be reprogrammed in P. cruentum. The result demonstrated that nitrate assimilation (with related unigenes of 8–493 fold upregulation) would be strengthen and photosynthesis (with related unigenes of 6–35 fold downregulation) be impaired under nitrogen deprivation. Importantly, compared to other green algae, red microalga P. cruentum presented a different expression pattern of lipid metabolism in response to nitrogen stress. These observations will also provide novel insight for understanding adaption mechanisms and potential targets for metabolic engineering and synthetic biology in P. cruentum.

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The chloroplast genome of the marine microalga Tisochrysis lutea

Mitochondrial DNA Part B ◽

10.1080/23802359.2018.1547140 ◽

2019 ◽

Vol 4 (1) ◽

pp. 253-255 ◽

Cited By ~ 3

Author(s):

Alejandra B. Méndez-Leyva ◽

Jingya Guo ◽

Elisabeth A. Mudd ◽

Jerry Wong ◽

Jean-Marc Schwartz ◽

...

Keyword(s):

Chloroplast Genome ◽

Tisochrysis Lutea ◽

Marine Microalga

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Characterization of Iodine-Related Molecular Processes in the Marine Microalga Tisochrysis lutea (Haptophyta)

Frontiers in Marine Science ◽

10.3389/fmars.2018.00134 ◽

2018 ◽

Vol 5 ◽

Cited By ~ 1

Author(s):

Laura Hernández Javier ◽

Hicham Benzekri ◽

Marta Gut ◽

M. Gonzalo Claros ◽

Stefanie van Bergeijk ◽

...

Keyword(s):

Molecular Processes ◽

Tisochrysis Lutea ◽

Marine Microalga

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Comparative Transcriptome of Wild Type and Selected Strains of the Microalgae Tisochrysis lutea Provides Insights into the Genetic Basis, Lipid Metabolism and the Life Cycle

PLoS ONE ◽

10.1371/journal.pone.0086889 ◽

2014 ◽

Vol 9 (1) ◽

pp. e86889 ◽

Cited By ~ 33

Author(s):

Gregory Carrier ◽

Matthieu Garnier ◽

Loïc Le Cunff ◽

Gaël Bougaran ◽

Ian Probert ◽

...

Keyword(s):

Life Cycle ◽

Lipid Metabolism ◽

Genetic Basis ◽

Comparative Transcriptome ◽

Wild Type ◽

Tisochrysis Lutea

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Different DHA or EPA production responses to nutrient stress in the marine microalga Tisochrysis lutea and the freshwater microalga Monodus subterraneus

The Science of The Total Environment ◽

10.1016/j.scitotenv.2018.11.346 ◽

2019 ◽

Vol 656 ◽

pp. 140-149 ◽

Cited By ~ 8

Author(s):

Hao Hu ◽

Jia-Yun Li ◽

Xin-Rong Pan ◽

Fang Zhang ◽

Lin-Lin Ma ◽

...

Keyword(s):

Nutrient Stress ◽

Tisochrysis Lutea ◽

Marine Microalga ◽

Monodus Subterraneus

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Co-production of fucoxanthin, docosahexaenoic acid (DHA) and bioethanol from the marine microalga Tisochrysis lutea

Biochemical Engineering Journal ◽

10.1016/j.bej.2021.108160 ◽

2021 ◽

pp. 108160

Author(s):

Malith Premaratne ◽

Vinoj Chamilka Liyanaarachchi ◽

Pemaththu Hewa Viraj Nimarshana ◽

Thilini U. Ariyadasa ◽

Anushree Malik ◽

...

Keyword(s):

Docosahexaenoic Acid ◽

Tisochrysis Lutea ◽

Marine Microalga

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Anti-Hepatocellular Carcinoma (HepG2) Activities of Monoterpene Hydroxy Lactones Isolated from the Marine Microalga Tisochrysis Lutea

Marine Drugs ◽

10.3390/md18110567 ◽

2020 ◽

Vol 18 (11) ◽

pp. 567

Author(s):

Katkam N. Gangadhar ◽

Maria João Rodrigues ◽

Hugo Pereira ◽

Helena Gaspar ◽

F. Xavier Malcata ◽

...

Keyword(s):

Hepg2 Cells ◽

Preparative Thin Layer Chromatography ◽

Omega 3 ◽

Nmr Analysis ◽

Tisochrysis Lutea ◽

Marine Microalga ◽

Bioassay Guided Fractionation ◽

First Time ◽

Layer Chromatography

Tisochrysis lutea is a marine haptophyte rich in omega-3 polyunsaturated fatty acids (e.g., docosahexaenoic acid (DHA)) and carotenoids (e.g., fucoxanthin). Because of the nutraceutical applications of these compounds, this microalga is being used in aquaculture to feed oyster and shrimp larvae. In our earlier report, T. lutea organic crude extracts exhibited in vitro cytotoxic activity against human hepatocarcinoma (HepG2) cells. However, so far, the compound(s) accountable for the observed bioactivity have not been identified. Therefore, the aim of this study was to isolate and identify the chemical component(s) responsible for the bioactivity observed. Bioassay-guided fractionation through a combination of silica-gel column chromatography, followed by preparative thin layer chromatography (PTLC), led to the isolation of two diastereomers of a monoterpenoid lactone, namely, loliolide (1) and epi-loliolide (2), isolated for the first time in this species. The structural elucidation of both compounds was carried out by GC-MS and 1D (1H and 13C APT) and 2D (COSY, HMBC, HSQC-ed, and NOESY) NMR analysis. Both compounds significantly reduced the viability of HepG2 cells and were considerably less toxic towards a non-tumoral murine stromal (S17) cell line, although epi-loliolide was found to be more active than loliolide.

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