Acid whey permeate: An alternative growth medium for microalgae Tetradesmus obliquus and production of β-galactosidase

Introduction. Microorganisms of dairy raw materials tend to adhere to the surfaces of processing equipment and form sustainable biofilms, which is a serious issue in the dairy industry. The goal of the present work was to investigate formation of biofilms on a glass surface in static model conditions, and removal of such biofilms by cleaning. Study objects and methods. The study objects were the permeates of skim milk, sweet whey and acid whey, as well as the biofilms formed and washings from glass slides. Biofilms were removed from the glass with detergents used in the dairy industry. Standard methods of determining microbiological and physicochemical properties were used to characterize the permeates. The biofilm structure and morphology of microorganisms participating in biofilm formation were investigated with a light spectroscopy. The efficiency of biofilm removal in a cleaning process was quantified with optical density of washings. Results and discussion. Biofilms in whey permeates formed slower compared to those in skimmed milk permeate during the first 24 h. Yeasts contributed significantly to the biofilm microflora in acid whey permeate throughout 5 days of biofilm growth. Well adhered biofilm layers were the most stable in skimmed milk permeate. The highest growth of both well and poorly adhered biofilm layers was observed in sweet whey permeate after 3–5 days. It was established that the primary attachment of microorganisms to a glass surface occurred within 8 h, mature multicultural biofilms formed within 48 h, and their partial destruction occurred within 72 h. Conclusion. The research results can be used to improve the cleaning equipment procedures in processing secondary dairy raw materials.

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One-Pot Synthesis of Lactose Derivatives from Whey Permeate

Foods ◽

10.3390/foods9060784 ◽

2020 ◽

Vol 9 (6) ◽

pp. 784 ◽

Cited By ~ 1

Author(s):

Maryam Enteshari ◽

Sergio I. Martínez-Monteagudo

Keyword(s):

Organic Acids ◽

Maximum Yield ◽

Continuous Stirred Tank Reactor ◽

One Pot ◽

Whey Permeate ◽

One Pot Synthesis ◽

Maximum Conversion ◽

Acid Whey ◽

Continuous Stirred Tank ◽

The One

The simultaneous production of lactulose (LAU), lactobionic acid (LBA), and organic acids from sweet and acid whey permeate (SWP and AWP) via catalytic synthesis (5% Ru/C) was studied in a continuous stirred-tank reactor. At selected conditions (60 °C, 60 bar, and 600 rpm), a maximum conversion of lactose (37 and 34%) was obtained after 90 min for SWP and AWP, respectively. The highest yield calculated with respect to the initial concentration of lactose for LAU was 22.98 ± 0.81 and 15.29 ± 0.81% after only 30 min for SWP, and AWP, respectively. For LBA, a maximum yield was found in SWP (5.23%) after 210 min, while about 2.2% was found in AWP. Six major organic acids (gluconic, pyruvic, lactic, formic, acetic, and citric acid) were quantified during the one-pot synthesis of lactose.

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Influence of sweet whey permeate utilization on Tetradesmus obliquus growth and β‐galactosidase production

The Canadian Journal of Chemical Engineering ◽

10.1002/cjce.24245 ◽

2021 ◽

Author(s):

Jihed Bentahar ◽

Jean‐Sébastien Deschênes

Keyword(s):

Whey Permeate ◽

Sweet Whey ◽

Tetradesmus Obliquus

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Evidence of the production of galactooligosaccharide from whey permeate by the microalgae Tetradesmus obliquus

Algal Research ◽

10.1016/j.algal.2019.101470 ◽

2019 ◽

Vol 39 ◽

pp. 101470 ◽

Cited By ~ 3

Author(s):

Shyam Suwal ◽

Jihed Bentahar ◽

Alice Marciniak ◽

Lucie Beaulieu ◽

Jean-Sébastien Deschênes ◽

...

Keyword(s):

Whey Permeate ◽

Tetradesmus Obliquus

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The Novel Solution for Acid Whey Permeate Application in Animal Feeding

Rural Sustainability Research ◽

10.2478/plua-2020-0011 ◽

2020 ◽

Vol 44 (339) ◽

pp. 1-7

Author(s):

Jana Lakstina ◽

Inese Aboltina ◽

Liga Vanaga ◽

Inga Ciprovica ◽

Daina Jonkus ◽

...

Keyword(s):

Cell Count ◽

Milk Yield ◽

Milk Fat ◽

Somatic Cell Count ◽

Milk Composition ◽

Quality Data ◽

Whey Permeate ◽

Lactating Cows ◽

Acid Whey ◽

Experimental Group

AbstractThe experiment was conducted to analyse the effect of fermented acid whey permeate on milk yield and composition in the lactating cows. Propionic acid bacteria and their metabolites have been used in the lactating cows feeding over decades, primarily to improve growth performance, feed conversation and milk production efficiency. Two groups of the lactating cows were arranged in the study: control group (n=50) and experimental group (n=50). Experimental group’s animals received 0.5 L of fermented whey permeate daily. Acid whey permeate was inoculated with the freeze-dried PS-4 (Propionibacterium freudenreichii subsp. shermanii, Chr.Hansen, Denmark) starter and fermented anaerobically for 48 hours at 20±2 oC. Fat, protein, lactose and total solids concentration in acid whey permeate and fermented acid whey permeate was analysed by the standard methods, but propionic acid was detected by HPLC. Milk composition and quality indices were determined at the beginning of the study and each month during 6 months period. At the end of the study the feeding of fermented acid whey permeate was stopped, but milk composition and quality data were monitored additionally after one month. Milk fat, protein, lactose, total solids, urea concentration and somatic cell count were analysed by a near infrared spectroscopy.The variability in milk composition and quality data across trial was greater in the experiment group than in the control. Milk fat and somatic cell count were significantly different (p<0.05) than other studied parameters in the experimental group cows’ milk. Milk yield and lactose concentration were tended to increase during feeding of fermented acid whey permeate in the lactating cows without significant differences between control and experimental groups. Fermented acid whey permeate as feed supplement improves energy metabolism for dairy cows which results in the higher milk yield and fat concentration.

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