scholarly journals Year-long evaluation of microalgae production in wastewater using pilot-scale raceway photobioreactors: Assessment of biomass productivity and nutrient recovery capacity

2021 ◽  
Vol 60 ◽  
pp. 102500
Author(s):  
Ainoa Morillas-España ◽  
Tomás Lafarga ◽  
Ana Sánchez-Zurano ◽  
Francisco Gabriel Acién-Fernández ◽  
Enrique Rodríguez-Miranda ◽  
...  
Keyword(s):  
Biomass Productivity ◽  
Pilot Scale ◽  
Nutrient Recovery ◽  
Recovery Capacity

scholarly journals Annual assessment of the wastewater treatment capacity of the microalga Scenedesmus almeriensis and optimisation of operational conditions

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ana Sánchez-Zurano ◽  
Ainoa Morillas-España ◽  
Cintia Gómez-Serrano ◽  
Martina Ciardi ◽  
Gabriel Acién ◽  
...  
Keyword(s):  
Dilution Rate ◽  
Biomass Productivity ◽  
Pilot Scale ◽  
Nutrient Recovery ◽  
Urban Wastewater ◽  
Operational Conditions ◽  
Recovery Capacity ◽  
Lower Volume ◽  
The Relationship ◽  
Striking Effect

AbstractThe depth of the culture and the dilution rate have a striking effect on the biomass productivity and the nutrient recovery capacity of microalgal cultures. The combination of culture depth and dilution rate that allows to maximise the performance of the system depends on environmental conditions. In the current study, a response surface methodology was used to explore the relationship between the two most relevant operational conditions and the biomass productivity achieved in 8.3 m2 pilot-scale raceways operated using urban wastewater. Four polynomial models were developed, one for each season of the year. The software predicted biomass productivities of 12.3, 25.6, 32.7, and 18.9 g·m−2·day−1 in winter, spring, summer, and autumn, respectively. The models were further validated at pilot-scale with R2 values ranging within 0.81 and 0.91, depending on the season. Lower culture depths had the advantage of minimising nitrification and stripping but allow to process a lower volume of wastewater per surface area. Biomass productivity was higher at culture depths of 0.05 m, when compared to 0.12 and 0.20 m, while the optimal dilution rate was season-dependent. Results reported herein are useful for optimising the biomass productivity of raceway reactors located outdoors throughout the year.

scholarly journals Growth of Haematococcus pluvialis on a Small-Scale Angled Porous Substrate Photobioreactor for Green Stage Biomass

2021 ◽  
Vol 11 (4) ◽  
pp. 1788
Author(s):  
Thanh-Tri Do ◽  
Binh-Nguyen Ong ◽  
Tuan-Loc Le ◽  
Thanh-Cong Nguyen ◽  
Bich-Huy Tran-Thi ◽  
...  
Keyword(s):  
Light Intensity ◽  
Algal Biomass ◽  
Haematococcus Pluvialis ◽  
Biomass Productivity ◽  
Pilot Scale ◽  
Small Scale ◽  
Porous Substrate ◽  
Low Light ◽  
Green Algal ◽  
Green Stage

In the production of astaxanthin from Haematococcus pluvialis, the process of growing algal biomass in the vegetative green stage is an indispensable step in both suspended and immobilized cultivations. The green algal biomass is usually cultured in a suspension under a low light intensity. However, for astaxanthin accumulation, the microalgae need to be centrifuged and transferred to a new medium or culture system, a significant difficulty when upscaling astaxanthin production. In this research, a small-scale angled twin-layer porous substrate photobioreactor (TL-PSBR) was used to cultivate green stage biomass of H. pluvialis. Under low light intensities of 20–80 µmol photons m−2·s−1, algae in the biofilm consisted exclusively of non-motile vegetative cells (green palmella cells) after ten days of culturing. The optimal initial biomass density was 6.5 g·m−2, and the dry biomass productivity at a light intensity of 80 µmol photons m−2·s−1 was 6.5 g·m−2·d−1. The green stage biomass of H. pluvialis created in this small-scale angled TL-PSBR can be easily harvested and directly used as the source of material for the inoculation of a pilot-scale TL-PSBR for the production of astaxanthin.

scholarly journals Influence of Light Intensity and Temperature on Cultivation of Microalgae Desmodesmus Communis in Flasks and Laboratory-Scale Stirred Tank Photobioreactor

2015 ◽  
Vol 52 (2) ◽  
pp. 59-70 ◽  
Author(s):  
J. Vanags ◽  
L. Kunga ◽  
K. Dubencovs ◽  
V. Galvanauskas ◽  
O. Grīgs
Keyword(s):  
Light Intensity ◽  
Shake Flask ◽  
Scale Up ◽  
Biomass Concentration ◽  
Biomass Productivity ◽  
Pilot Scale ◽  
Laboratory Scale ◽  
Stirred Tank ◽  
Light Limitation ◽  
Maximum Biomass

Abstract Optimization of the microalgae cultivation process and of the bioprocess in general traditionally starts with cultivation experiments in flasks. Then the scale-up follows, when the process from flasks is transferred into a laboratory-scale bioreactor, in which further experiments are performed before developing the process in a pilot-scale reactor. This research was done in order to scale-up the process from a 0.4 1 shake flask to a 4.0 1 laboratory-scale stirred-tank photobioreactor for the cultivation of Desmodesmus (D.) communis microalgae. First, the effect of variation in temperature (21-29 ºC) and in light intensity (200-600 μmol m-2s-1) was studied in the shake-flask experiments. It was shown that the best results (the maximum biomass concentration of 2.72 g 1-1 with a specific growth rate of 0.65 g g-1d-1) can be achieved at the cultivation temperature and light intensity being 25 °C and 300 μmol m2s-1, respectively. At the same time, D. communis cultivation under the same conditions in stirred-tank photobioreactor resulted in average volumetric productivities of biomass due to the light limitation even when the light intensity was increased during the experiment (the maximum biomass productivity 0.25 g 1-1d-1; the maximum biomass concentration 1.78 g 1-1).

scholarly journals Nutrient recovery and fractionation of anaerobic digester effluents employing pilot scale membrane technology

2019 ◽  
Vol 31 ◽  
pp. 100846 ◽  
Author(s):  
Myrto-Panagiota Zacharof ◽  
Stephen J. Mandale ◽  
Darren Oatley-Radcliffe ◽  
Robert W. Lovitt
Keyword(s):  
Pilot Scale ◽  
Anaerobic Digester ◽  
Membrane Technology ◽  
Nutrient Recovery

scholarly journals Photosynthetic biogas upgrading to bio-methane: Boosting nutrient recovery via biomass productivity control

2016 ◽  
Vol 17 ◽  
pp. 46-52 ◽  
Author(s):  
Alma Toledo-Cervantes ◽  
Mayara L. Serejo ◽  
Saúl Blanco ◽  
Rebeca Pérez ◽  
Raquel Lebrero ◽  
...  
Keyword(s):  
Biomass Productivity ◽  
Nutrient Recovery ◽  
Biogas Upgrading

The potential of nutrient reuse from a source-separated domestic wastewater system in Indonesia – Case study: ecological sanitation pilot plant in Surabaya

2007 ◽  
Vol 56 (5) ◽  
pp. 141-148 ◽  
Author(s):  
A.F. Malisie ◽  
M. Prihandrijanti ◽  
R. Otterpohl
Keyword(s):  
Small Volume ◽  
Domestic Wastewater ◽  
Pilot Scale ◽  
The Other ◽  
Nutrient Recovery ◽  
Ecological Sanitation ◽  
Faecal Matter ◽  
Nutrient Reuse ◽  
Cultivation Experiment

Human excreta (faeces and urine) contribute only a small volume in domestic wastewater but are one of the main causes of water pollution. On the other hand, they contain very valuable nutrients to be reused as anthropogenic fertiliser through proper collection, treatment and hygienisation processes. To know the potential of nutrient recovery and reuse in Indonesia, a pilot scale source separation domestic wastewater system has been built in Surabaya and, so far, has shown promising results. Using urine diverting toilets, up to 86% nitrogen, 21% phosphorus and 69% potassium from urine and 12% of nitrogen, 68% of phosphorus and 20% of potassium from the faecal matter can be recovered. The separated urine was stored for 6 months before usage as fertiliser for hygienic reasons, while the separated faecal matter was composted with worms (vermicomposting). In order to investigate the fertilising effect, a preliminary cultivation experiment has been carried out on young rose plants using different fertilisers for 2 months.

scholarly journals Production of Scenedesmus almeriensis Using Pilot-Scale Raceway Reactors Located inside a Greenhouse

Phycology ◽  
2022 ◽  
Vol 2 (1) ◽  
pp. 76-85
Author(s):  
Ainoa Morillas-España ◽  
Silvia Villaró ◽  
Martina Ciardi ◽  
Gabriel Acién ◽  
Tomás Lafarga
Keyword(s):  
Stationary Phase ◽  
Functional Foods ◽  
Biomass Productivity ◽  
Pilot Scale ◽  
Average Value ◽  
Industrial Sectors ◽  
Potential Applications ◽  
Agricultural Biostimulant ◽  
Macromolecular Composition ◽  
Scenedesmus Almeriensis

The microalga S. almeriensis was produced in spring and summer using 80 m2 raceway reactors located inside a greenhouse in Almería, Spain. This microalga was selected because it is a fast-growing and robust strain with potential applications in the production of functional foods and feeds or as a high-value agricultural biostimulant. Overall, the biomass productivity obtained in summer was 24.9 ± 0.9 g·m−2·day−1. This value was higher than that obtained in spring, with an average value of 21.4 ± 1.3 g·m−2·day−1 (p < 0.05). The Fv/Fm value of the cultures at the stationary phase was comparable and around 0.6, which is the optimum of this strain. No major differences in the macromolecular composition of the biomass were observed between seasons, with an average protein, lipid, ash, and carbohydrate content of 37.9, 4.6, 10.8, and 46.7%, respectively. The data reported herein were used to validate a previously described model. The differences between the experimental and the predicted biomass productivities were below 5% in spring and 8% in summer, although a larger dataset is needed to validate the model. Overall, results supported the robustness of the selected strain and its utilisation in different industrial sectors.

scholarly journals Pilot-scale phycoremediation using Muriellopsis sp. for wastewater reclamation in the Atacama Desert: microalgae biomass production and pigment recovery

2020 ◽  
Author(s):  
L. Cavieres ◽  
J. Bazaes ◽  
P. Marticorena ◽  
K. Riveros ◽  
P. Medina ◽  
...  
Keyword(s):  
Irrigation Water ◽  
Municipal Wastewater ◽  
Bubble Column ◽  
Atacama Desert ◽  
Biomass Productivity ◽  
Chlorella Pyrenoidosa ◽  
Pilot Scale ◽  
Wastewater Reclamation ◽  
Nitrogen And Phosphorus ◽  
Bubble Column Reactors

Abstract Municipal wastewater phycoremediation represents a promising circular economy-based process for wastewater reclamation used to recover water and produce biomass. This study aimed to evaluate a pilot-scale phycoremediation system, using the most efficient strain of microalgae for wastewater reclamation in the Atacama Desert. Nitrogen and phosphorus removal, as well as biomass growth, were compared in different microalgae treatments, namely Muriellopsis sp., Scenedesmus almeriensis, Chlamydomonas segnis, Chlorella pyrenoidosa and Chlorella vulgaris. The most efficient treatments, Muriellopsis sp. and S. almeriensis, were scaled up to 20-L bubble column reactors to evaluate nutrient removal and biomass biochemical profile for potential biotechnological application. Finally, Muriellopsis sp. was selected for a pilot-scale phycoremediation experiment (800-L raceway), which removed 84% of nitrogen, 93% of phosphorus and other chemical compounds after 4 days of treatment to meet most of the Chilean standards for irrigation water (NCh. 1333. DS. MOP No. 867/78). Faecal coliforms count was reduced by 99.9%. Furthermore, biomass productivity reached 104.25 mg·L–1·day–1 value with 51% protein, and pigment content of 0.6% carotenoid, with 0.3% lutein. These results indicate the potential of wastewater phycoremediation at an industrial scale for the production of irrigation water and carotenoid using Muriellopsis sp.

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