scholarly journals The effect of Platelet-Rich Plasma and Stromal Vascular Fraction combination on Epidermal Growth Factor serum level for anal trauma healing in the Wistar rat model

2021 ◽  
Vol 70 ◽  
pp. 102773
Author(s):  
Ivanna Sirowanto ◽  
Fonny Josh ◽  
Sulmiati ◽  
Ahmadwirawan ◽  
Andi Alfian Zainuddin ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Serum Level ◽  
Rat Model ◽  
Platelet Rich Plasma ◽  
Stromal Vascular Fraction ◽  
Wistar Rat ◽  
Epidermal Growth ◽  
Trauma Healing

scholarly journals Epidermal growth factor or platelet-rich plasma combined with induced membrane technique in the treatment of segmental femur defects: an experimental study

2020 ◽  
Vol 15 (1) ◽  
Author(s):  
Ökkeş Bilal ◽  
Duran Topak ◽  
Mustafa Kınaş ◽  
Ergül Belge Kurutaş ◽  
Betül Kızıldağ ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Rat Model ◽  
Platelet Rich Plasma ◽  
Bone Defects ◽  
Control Group ◽  
Therapeutic Benefits ◽  
Masquelet Technique ◽  
Membrane Technique ◽  
Epidermal Growth

Abstract Objective Extensive bone defects remain a therapeutic challenge necessitating alternative surgical approaches with better outcomes. Can increase the effectiveness of PRP or EGF treatment in surgical treatment of large bone defects with Masquelet technique? Aim of this study examined potential therapeutic benefits of the Masquelet technique with induced membranes in combination with platelet-rich plasma (PRP) or epidermal growth factor (EGF) in a rat model of segmental femur defect. Methods Three groups each consisting of 20 Sprague-Dawley rats were defined as follows: EGF group, PRP group, and control group. A femoral bone defect was created and filled with antibiotic embedded polymethyl methacrylate. Half of the animals in each group were sacrificed at week 6 and the pseudo-membranes formed were analyzed. In the remaining half, the cement was removed and the space was filled with autograft. After another 6 weeks, the structures formed were examined radiologically, histologically, and biochemically. Results At week 6, both PRP and EGF groups had significantly higher membrane CD31, TGF-beta, and VEGF levels than controls. At week 12, when compared to controls, PRP and EGF groups had significantly higher membrane CD31 levels and the PRP group had significantly higher membrane TGF levels. Regarding bone tissue levels, PRP and EGF groups had significantly higher VEGF levels and the EGF group had significantly higher BMP levels. In addition, PRP and EGF groups had higher radiological scores than controls. However, the two experimental groups did not differ with respect to any parameter tested in this study. Conclusion Both PRP and EGF seem to be associated with histological, biochemical, and radiological improvements in experimental rat model of Masquelet technique, warranting in further clinical studies. Level of evidence Level 5

scholarly journals Epidermal Growth Factor or Platelet Rich Plasma Combined With Induced Membrane Technique in the Treatment of Segmental Femur Defects: An Experimental Study

2020 ◽  
Author(s):  
Ökkeş Bilal ◽  
Duran Topak ◽  
Mustafa Kınaş ◽  
Ergül Belge Kurutaş ◽  
Betül Kızıldağ ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Rat Model ◽  
Platelet Rich Plasma ◽  
Bone Defects ◽  
Control Group ◽  
Therapeutic Benefits ◽  
Masquelet Technique ◽  
Membrane Technique ◽  
Epidermal Growth

Abstract Objective: Extensive bone defects remain a therapeutic challenge necessitating alternative surgical approaches with better outcomes. Can increase the effectiveness of PRP or EGF treatment in surgical treatment of large bone defects with masquelet technique?Aim of this study examined potential therapeutic benefits of the Masquelet technique with induced membranes in combination with platelet rich plasma (PRP) or epidermal growth factor (EGF) in a rat model of segmental femur defect.Methods: Three groups each consisting of 20 Sprague-Dawley rats were defined as follows: EGF group, PRP group, and control group. A femoral bone defect was created and filled with antibiotic embedded polymethyl metacrylate. Half of the animals in each group were sacrificed at week 6 and the pseudo-membranes formed were analyzed. In the remaining half, the cement was removed and the space was filled with autograft. After another six weeks, the structures formed were examined radiologically, histologically and biochemically.Results: At week 6, both PRP and EGF groups had significantly higher membrane CD31, TGF and VEGF levels than controls. At week 12, when compared to controls, PRP and EGF groups had significantly higher membrane CD31 levels and PRP group had significantly higher membrane TGF levels. Regarding bone tissue levels, PRP and EGF groups had significantly higher VEGF levels and EGF group had significantly higher BMP levels. In addition, PRP and EGF groups had higher radiological scores than controls. However, the two experimental groups did not differ with respect to any parameter tested in this study. Conclusion: Both PRP and EGF seems to be associated with histological, biochemical, and radiological improvements in experimental rat model of Masquelet technique, warranting in further clinical studies.

Epidermal growth factor reduces the development of necrotizing enterocolitis in a neonatal rat model

2002 ◽  
Vol 282 (1) ◽  
pp. G156-G164 ◽  
Author(s):  
Bohuslav Dvorak ◽  
Melissa D. Halpern ◽  
Hana Holubec ◽  
Catherine S. Williams ◽  
Debra L. McWilliam ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Mrna Expression ◽  
Necrotizing Enterocolitis ◽  
Rat Model ◽  
Transforming Growth Factor ◽  
Gastrointestinal Disease ◽  
Neonatal Rat ◽  
Epidermal Growth ◽  
Neonatal Rat Model

Necrotizing enterocolitis (NEC) is the most common gastrointestinal disease of prematurely born infants. Maternal milk plays an important protective role against NEC development and is the major source of epidermal growth factor (EGF) for neonates. The aim of this study was to examine the effect of orally administered EGF on the incidence of NEC in a neonatal rat model. Newborn rats were artificially fed either with growth factor-free rat milk substitute (RMS) or RMS supplemented with 500 ng/ml of EGF (RMS+EGF). Experimental NEC was induced by exposure to asphyxia and cold stress. Development of NEC was evaluated by gross and histological scoring of damage in the ileum. Ileal EGF receptor (EGF-R), EGF, and transforming growth factor-α mRNA expression was assessed by RT competitive-PCR, and the EGF-R was localized by immunohistochemistry. EGF supplementation of formula reduced the incidence and severity of NEC in rats (13/16 RMS vs. 4/13 RMS+EGF). Ileal EGF-R mRNA expression was markedly increased in the RMS group compared with RMS+EGF. Enhanced EGF-R expression in the RMS group was localized predominantly in the epithelial cells of injured ileum. These data suggest a new potential therapeutic approach for the prevention and treatment of NEC.

SERUM LEVEL OF EPIDERMAL GROWTH FACTOR RECEPTOR AS A BIOMARKER OF LIVER CANCER

Epidemiology ◽  
2004 ◽  
Vol 15 (4) ◽  
pp. S71
Author(s):  
How-Ran Guo ◽  
Tzu-I Sung ◽  
Ying-Jan Wang ◽  
Tsui-Lien Hung ◽  
Chi-Yi Chen
Keyword(s):  
Epidermal Growth Factor Receptor ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Serum Level ◽  
Liver Cancer ◽  
Growth Factor Receptor ◽  
Epidermal Growth

Synergistic effects of systemic trefoil factor family 1 (TFF1) peptide and epidermal growth factor in a rat model of colitis

Peptides ◽  
2004 ◽  
Vol 25 (5) ◽  
pp. 793-801 ◽  
Author(s):  
A.J FitzGerald ◽  
M Pu ◽  
T Marchbank ◽  
B.R Westley ◽  
F.E.B May ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Rat Model ◽  
Synergistic Effects ◽  
Trefoil Factor ◽  
Epidermal Growth ◽  
Trefoil Factor Family

scholarly journals Intrauterine Infusion of Autologous Platelet-Rich Plasma Affects the Endometrial Thickness, Epidermal Growth Factor and Pregnancy Outcome in Patients Undergoing IUI

2020 ◽  
Vol 9 (2) ◽  
Author(s):  
Wafaa A. Abaid ◽  
◽  
Manal T. Al-Obaidi ◽  
Muayad S. Abood ◽  
◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Endometrial Thickness ◽  
Platelet Rich Plasma ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Epidermal Growth ◽  
Autologous Platelet Rich Plasma ◽  
The Mean ◽  
Autologous Platelet

Despite developments in assisted reproductive technology, there is immaterial progress in the implantation and pregnancy rates. Intrauterine infusion (IUIF) of autologous platelet-rich plasma (PRP) might renew implantation rates through its paracrine properties by progression cytokines and growth factors which favor implantation. Here we determine whether the IUIF of autologous PRP had a role in pregnancy outcome through its outcome on epidermal growth factor and endometrial thickness. An overall of 43 patients where prospectively randomly dispersed into two groups subjected to a superovulation program using Letrozole® tablet orally 2.5 mg twice daily 12 hours apart from day 2 for 5 days for one cycle. 20 women were considered as control receiving the conventional intrauterine insemination (IUI) management while 23 of them were given PRP by IUIF on the day of human chorionic gonadotrophin injection. The IUI was done for both groups 36-48 hours after confirming ovulation. The blood samples were collected from both groups on the day of IUI for the valuation of epidermal growth factor and an ultrasound was done on the day of human chorionic gonadotrophin injection and day of IUI for assessment of endometrial thickness. The mean endometrial thickness in the PRP group at the day of IUI was significantly thicker than that of the control group and the difference in percentage change of endometrial thickness between PRP group and controls significantly higher in PRP group. The mean epidermal growth factor and the pregnancy rate were significantly superior in the PRP group than that of controls. In conclusion, autologous PRP IUIF was well-tolerated and resulted in a significant expansion in endometrial thickness, epidermal growth factor Level and, subsequent pregnancy rate in an infertile woman undergoing IUI.

The effects of epidermal growth factor embedded dressing on wound healing in rat model

2013 ◽  
Author(s):  
Yoon Taek Koo ◽  
Hak Chang ◽  
Kyung Won Minn ◽  
Jong Shin Park ◽  
Hyo Jin Park
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Rat Model ◽  
Epidermal Growth
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