Evaluation of a nucleic acid amplification assay for the diagnosis of Clostridioides difficile infection

Anaerobe ◽  
2019 ◽  
Vol 59 ◽  
pp. 201-204
Author(s):  
Ozlem Koyuncu-Ozyurt ◽  
Betil Ozhak ◽  
Dilara Ogunc ◽  
Gozde Ongut ◽  
Filiz Gunseren ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Amplification ◽  
Clostridioides Difficile ◽  
Clostridioides Difficile Infection ◽  
Nucleic Acid Amplification Assay ◽  
Amplification Assay

scholarly journals Comparison of Different Methods of RNA Preparation from Peripheral Blood for Nucleic Acid Amplification Assay

2018 ◽  
Vol 36 (1) ◽  
pp. 77-82 ◽  
Author(s):  
Dong Eun Lee ◽  
Hanna Lee ◽  
Seon Duk Lee ◽  
Hyung Soo Han ◽  
Jae Young Choe ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Peripheral Blood ◽  
Nucleic Acid Amplification ◽  
Nucleic Acid Amplification Assay ◽  
Amplification Assay

scholarly journals 2356. Increased Clinical Specificity with Ultrasensitive Detection of Clostridioides difficile Toxins: Reduction of Overdiagnosis Compared with Nucleic Acid Amplification Tests

2019 ◽  
Vol 6 (Supplement_2) ◽  
pp. S811-S812 ◽  
Author(s):  
Johanna Sandlund ◽  
Joel Estis ◽  
Phoebe Katzenbach ◽  
Niamh Nolan ◽  
Kirstie Hinson ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Disease Severity ◽  
Single Molecule ◽  
Clinical Performance ◽  
Neutralization Assay ◽  
Nucleic Acid Amplification ◽  
Clostridioides Difficile ◽  
Percent Agreement ◽  
Clostridioides Difficile Infection ◽  
Healthcare Associated

Abstract Background Clostridioides difficile infection (CDI) is one of the most common healthcare-associated infections, resulting in significant morbidity, mortality, and economic burden. Diagnosis of CDI relies on the assessment of clinical presentation and laboratory tests. We have evaluated the clinical performance of ultrasensitive Single Molecule Counting technology for detection of C. difficile toxins A and B. Methods Stool specimens from 298 patients with suspected CDI were tested with nucleic acid amplification test (NAAT; BD MAX™ Cdiff assay or Xpert® C. difficile assay) and Singulex Clarity® C. difficile toxins A/B assay. Specimens with discordant results were tested with cell cytotoxicity neutralization assay (CCNA), and results were correlated with disease severity and outcome. Results There were 64 NAAT-positive and 234 NAAT-negative samples. Of the 32 NAAT+/Clarity− and 4 NAAT-/Clarity+ samples, there were 26 CCNA− and 4 CCNA- samples, respectively. CDI relapse or overall death was more common in NAAT+/toxin+ patients than in NAAT+/toxin− and NAAT−/toxin− patients, and NAAT+/toxin+ patients were 3.7 times more likely to experience relapse or death (Figure 1). The clinical specificity of Clarity and NAAT was 97.4% and 89.0%, respectively, and overdiagnosis was over three times more common in NAAT+/toxin− than in NAAT+/toxin+ patients (Figure 2). Negative percent agreement between NAAT and Clarity was 98.3%, and positive percent agreement increased from 50.0% to effective 84.2% and 94.1% after CCNA testing and clinical assessment. Conclusion The Clarity assay was superior to NAATs in diagnosis of CDI, by reducing overdiagnosis and thereby increasing clinical specificity, and presence of toxins was associated with disease severity and outcome. Disclosures All authors: No reported disclosures.

Performance of a new system using a one-step nucleic acid amplification assay for detecting lymph node metastases in breast cancer

2019 ◽  
Vol 36 (6) ◽  
Author(s):  
Kenzo Shimazu ◽  
Tomonori Tanei ◽  
Yasuhiro Tamaki ◽  
Toshiaki Saeki ◽  
Akihiko Osaki ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Lymph Node ◽  
Nucleic Acid ◽  
Lymph Node Metastases ◽  
Nucleic Acid Amplification ◽  
Nucleic Acid Amplification Assay ◽  
Amplification Assay ◽  
One Step ◽  
Node Metastases ◽  
New System

Non-instrumented nucleic acid amplification assay

2008 ◽  
Author(s):  
Bernhard H. Weigl ◽  
Gonzalo Domingo ◽  
Jay Gerlach ◽  
Dennis Tang ◽  
Darrel Harvey ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Amplification ◽  
Nucleic Acid Amplification Assay ◽  
Amplification Assay

scholarly journals Diagnostic potential of 1-step nucleic acid amplification assay in patients with head and neck squamous cell carcinoma based on CK19 expression in a primary lesion

Head & Neck ◽  
2015 ◽  
Vol 38 (S1) ◽  
pp. E239-E245 ◽  
Author(s):  
Masahiro Suzuki ◽  
Takashi Matsuzuka ◽  
Yuko Hashimoto ◽  
Masakazu Ikeda ◽  
Satoshi Saijo ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Nucleic Acid ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Primary Lesion ◽  
Nucleic Acid Amplification ◽  
Diagnostic Potential ◽  
Ck19 Expression ◽  
Nucleic Acid Amplification Assay ◽  
Amplification Assay

A one-tube quantitative HIV-1 RNA NASBA nucleic acid amplification assay using electrochemiluminescent (ECL) labelled probes

1994 ◽  
Vol 49 (2) ◽  
pp. 157-167 ◽  
Author(s):  
Bob van Gemen ◽  
Rinie van Beuningen ◽  
Arjan Nabbe ◽  
Dianne van Strijp ◽  
Suzanne Jurriaans ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Amplification ◽  
Nucleic Acid Amplification Assay ◽  
Amplification Assay ◽  
Hiv 1
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