Wheat bran addition improves Ceriporiopsis subvermispora and Lentinula edodes growth on wheat straw, but not delignification

2020 ◽  
Vol 259 ◽  
pp. 114361
Author(s):  
Lei Mao ◽  
John W. Cone ◽  
Wouter H. Hendriks ◽  
Anton S.M. Sonnenberg
2020 ◽  
Vol 236 ◽  
pp. 104013
Author(s):  
Lei Mao ◽  
J. Leon M. Marchal ◽  
Anton S.M. Sonnenberg ◽  
John W. Cone ◽  
Viviane Endo Hidalgo ◽  
...  

2017 ◽  
Vol 105 ◽  
pp. 381-391 ◽  
Author(s):  
Sandra J.A. van Kuijk ◽  
Anton S.M. Sonnenberg ◽  
Johan J.P. Baars ◽  
Wouter H. Hendriks ◽  
José C. del Río ◽  
...  

2020 ◽  
Vol 32 (1) ◽  
pp. 115-124
Author(s):  
Hasan Sardar ◽  
Muhammad Akbar Anjum ◽  
Aamir Nawaz ◽  
Safina Naz ◽  
Shaghef Ejaz ◽  
...  

AbstractAgricultural waste disposal is one of the main concerns in today's world that can cause environmental pollution. Utilisation of agro-waste materials to grow mushrooms is an eco-friendly method to reduce pollution. Therefore, various agricultural waste materials, such as wheat straw, rice straw and cotton waste, were utilised for the production of milky mushroom. Among the substrates used in this study wheat straw showed superior substrate for the production of milky mushroom. The agronomic traits studied such as total yield, number of fruiting bodies, the maximum diameter of pileus and stalk length, biological efficiency, protein contents, phosphorous and potassium contents were observed on wheat straw substrate. Peat moss, loam soil and spent mushroom substrate were used as casing materials. Among the casing materials used, the highest yield and biological efficiency were observed on peat moss. The results also indicated that the addition of supplements with the substrate improved yield and yield contributing characteristics. Among the tested supplements (wheat bran and rice bran), wheat bran was the best supplement for wheat straw substrate to cultivate milky white mushroom.


Author(s):  
Nataļja Matjuškova ◽  
Laura Okmane ◽  
Dzintra Zaļā ◽  
Linda Rozenfelde ◽  
Māris Puķe ◽  
...  

Abstract The effect of lignocellulose and lignin on growth of mycelium of mushroom Lentinula edodes and laccase activity in cultivation medium was studied. It was shown that cultivation of L. edodes mycelium in liquid nutrient medium with addition of 0.25-0.5% of kraft lignin increased mycelium biomass yield approximately two times compared with reference conditions without addition of lignin. Similar results were obtained in experiments in which 0.5% lignocellulose that remained after obtaining furfural, and 0.5% lignin that remained after obtaining furfural and glucose from wheat straw, were added to the nutrient medium. This effect was greater in the conditions of cultivation with good aeration, compared with static culture. Laccase activity in medium increased after addition of wheat straw lignocellulose or lignin only in the case of mycelium cultivation with aeration. In the case of mushroom cultivation on solid nutrient medium, addition of wheat straw lignocellulose and lignin promoted growth of mycelium only during the first 7 days of cultivation.


2021 ◽  
Vol 35 (4) ◽  
pp. 640-649
Author(s):  
Dalvan Pereira Abilio ◽  
Olívia Gomes Martins ◽  
Giovana Silva ◽  
Meire Andrade

PROSPECÇÃO E AVALIAÇÃO DO POTENCIAL BIOTECNOLÓGICO DE LINHAGENS COMERCIAIS DE SHIITAKE   DALVAN PEREIRA ABÍLIO1, OLÍVIA GOMES MARTINS2, GIOVANA CRISTINA PINTO ALVES DA SILVA3, MEIRE CRISTINA NOGUEIRA DE ANDRADE4   1 Graduando em Ciências Biológicas, Centro Universitário Sagrado Coração – Unisagrado, R. Irmã Arminda, 10-50 - Jardim Brasil, 17011-160 Bauru, SP, Brasil. E-mail: [email protected] 2 Doutoranda em Agronomia – Energia na Agricultura, Faculdade de Ciências Agronômicas, Universidade Estadual Paulista "Julio de Mesquita Filho" (UNESP), Av. Universitária, 3780 - Altos do Paraíso, 18610-034, Botucatu, SP, Brasil. E-mail: [email protected] 3 Mestre em Agronomia – Energia na Agricultura, Faculdade de Ciências Agronômicas, Universidade Estadual Paulista "Julio de Mesquita Filho" (UNESP), Av. Universitária, 3780 - Altos do Paraíso, 18610-034, Botucatu, SP, Brasil. E-mail: [email protected] 4 Docente da Faculdade Gran Tietê, Av. 15 de Novembro, 125 - Centro, 17340-000, Barra Bonita, SP, Brasil. E-mail: [email protected]   RESUMO: O objetivo deste trabalho foi avaliar e comparar o crescimento micelial in vitro de quatro linhagens comerciais de Lentinula edodes (LE-241, LE-242, LE-243 e LE-244) em meio de cultura à base de serragem de eucalipto, suplementado com bagaço de malte ou farelo de trigo. O delineamento experimental foi inteiramente casualizado, em esquema fatorial 2x4 (substratos x linhgens), totalizando oito tratamentos, cada um com cinco repetições. Os substratos foram preparados com 20% de suplementação, acrescidos de calcário (tamponante), umidificados e esterilizados. Os meios de cultura foram preparados a partir da infusão dos substratos, filtração, adição de ágar e esterilização. As placas foram inoculadas com as linhagens e o crescimento radial do micélio na superfície do meio de cultura foi mensurado com paquímetro. O menor desenvolvimento micelial ocorreu com a linhagem LE-241 e com a linhagem LE-243 no substrato suplementado com farelo de trigo. Os maiores desenvolvimentos foram obtidos com as linhagens LE-242 e LE-244 no substrado suplementado com bagaço de malte. O desenvolvimento de todas as linhagens foi maior no substrato suplementado com bagaço de malte. Sendo assim, a utilização do bagaço de malte para o crescimento micelial é uma alternativa viável à suplementação tradicional com farelo de trigo.   Palavras-chave: fungos, cogumelo, Lentinula edodes, micélio, bagaço de malte.   PROSPECTING AND EVALUATING THE BIOTECHNOLOGICAL POTENTIAL OF SHIITAKE COMMERCIAL STRAINS   ABSTRACT: This study aimed to evaluate and compare the in vitro mycelial growth of four commercial Lentinula edodes strains (LE-241, LE-242, LE-243 and LE-244) in a culture medium based on eucalyptus sawdust, supplemented with malt bagasse or wheat bran. The experimental design was completely randomized, in a 2x4 factorial scheme (substrates x strains), totaling eight treatments, each with five repetitions. The substrates were prepared with 20% supplementation, added with lime (buffer), humidified and sterilized. The culture media were prepared from the infusion of substrates, filtration, addition of agar and sterilization. The plates were inoculated with the strains and the radial growth of the mycelium on the surface of the culture medium was measured using a caliper. The smallest mycelial growth occurred with the LE-241 strain and with the LE-243 strain on the substrate supplemented with wheat bran. The greatest growths were obtained with the LE-242 and LE-244 strains in the substrate supplemented with malt bagasse. The development of all strains was greater in the substrate supplemented with malt bagasse. Therefore, the use of malt bagasse for mycelial growth is a viable alternative to traditional wheat bran supplementation.   Keywords: fungi, mushroom, Lentinula edodes, mycelium, malt bagasse.


2020 ◽  
Vol 60 (12) ◽  
pp. 1521
Author(s):  
Z. Iqbal ◽  
M. A. Rashid ◽  
T. N. Pasha ◽  
J. A. Bhatti

Current study evaluated the effects of feeding straw source and energy supplementation during prepartum period on postpartum production performance and changes in blood metabolites of crossbred dairy cows. Twenty-eight crossbred (Holstein × Sahiwal) cows were randomly assigned to one of the following four dietary treatments: (1) wheat straw and corn grain (WSCG), (2) wheat straw and wheat bran (WSWB), (3) oat straw and corn grain (OSCG) and (4) oat straw and wheat bran (OSWB) in a 2 × 2 factorial experiment. Iso-nitrogenous diets fed as ad libitum total mixed ration contained 25% wheat straw (WS) or oat straw (OS) and 10% corn grain (CG) or wheat bran (WB). Experimental duration was 42 days before and 56 days after calving. After calving, all animals were fed a similar lactation diet. Pre- and postpartum dry-matter intake % of BW was not affected by treatments. Prepartum energy balance (EBAL) was higher for OS than WS and higher for CG than WB animals. Postpartum EBAL was higher in the WSCG than OSCG treatment. Milk production and composition were not affected by straw source or energy concentrate. Milk yield at Weeks 1, 2 and 3 was higher in the WSWB than WSCG and OSWB treatments. Total solids and feed efficiency were higher in the WSWB than WSCG treatment. Postpartum plasma concentration of non-esterified fatty acids was higher in the WS than the OS diet; however, the measured values were within normal limits. Postpartum plasma β-hydroxybutyrate concentration was not affected by straw source or energy concentrate. Cows fed WSWB prepartum were in positive EBAL, before and after calving, closer to the recommended requirements. Moreover, cows fed WSWB had a lower incidence of health disorders and subclinical ketosis, higher milk production and better feed conversion efficiency during first 3 weeks after calving.


1960 ◽  
Vol 40 (2) ◽  
pp. 71-82 ◽  
Author(s):  
J. M. Bell

Wheat bran, wheat straw, alfalfa, oat hulls, beet pulp, corn cobs and cellulose were added to basal diets at levels; of 8, 16, 24 and 32 per cent and, in a second experiment, at six levels of digestible energy to permit approximately isocaloric comparisons in the range of 2.2 to 3.4 digestible kilocalories per gram of feed dry matter. All diets were designed to be nutritionally adequate on the basis of nutrient content per unit weight of diet. Weanling mice were fed the diets during 14-day growth tests.Digestibility coefficients for the energy fraction were obtained: bran 42; wheat straw 0; alfalfa 37; oat hulls 10; beet pulp 41; corn cobs 14, and cellulose 0 per cent. Estimations of the digestibility of the basal ration by regression methods indicated associative effects, with corn cobs depressing basal digestibility from 89 to 85 per cent and beet pulp, alfalfa and oat hulls depressing it to about 86 per cent.Varied responses were obtained to isocaloric diets depending on the bulk source. For example, on diets containing 2.2–2.4 digestible Calories/gm., mice fed diets containing wheat straw often failed to survive, those fed diets containing beet pulp or cellulose did poorly, but gains of 70 per cent of normal were obtained when wheat bran or oat hulls was the diluent.Relative rates of passage of ingesta were computed by a method involving consideration of feed intakes, energy digestibility, maintenance requirements and weight gains over a fixed period. Bran rations had the highest passage rates; wheat straw, alfalfa and beet pulp the lowest. In vitro measurements indicated that the latter feeds were least capable of swelling in water and presumably occupied less space in the stomach. However, wheat straw and alfalfa tended to retain their physical characteristics throughout digestion.The results of these experiments emphasize the complexity and the importance in non-ruminant nutrition of the fibrous or bulk components of the ration as they influence available energy, feed intake, volume of ingesta at various levels in the gastrointestinal tract, rate of passage and microbial activity.


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