Preparation of oriented collagen fiber scaffolds and its application in bone tissue engineering

2021 ◽  
Vol 22 ◽  
pp. 100902
Author(s):  
Chunyang Ma ◽  
Hetong Wang ◽  
Yongjie Chi ◽  
Yanling Wang ◽  
Le Jiang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Collagen Fiber ◽  
Fiber Scaffolds

scholarly journals Evaluation of the Osteoblast Behavior to PGA Textile Functionalized with RGD as a Scaffold for Bone Regeneration

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Mariné Ortiz ◽  
Diana María Escobar-Garcia ◽  
Marco Antonio Álvarez-Pérez ◽  
Amaury Pozos-Guillén ◽  
Christian Grandfils ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Laser Scanning ◽  
Tissue Injury ◽  
Polyglycolic Acid ◽  
Confocal Laser Scanning Microscope ◽  
Confocal Laser ◽  
Fiber Scaffolds

The new era of biomaterials for repairing bone tissue injury continues to be a challenge in bone tissue engineering. The fiber scaffolds allow for cellular interconnection and a microenvironment close to the bone extracellular matrix. The aim of this study was to evaluate the osteoblast behavior on a 3D textile of PGA (polyglycolic acid) fibers functionalized with the RGD (R: arginine; G: glycine; D: aspartic acid) peptide. The cell morphology, proliferation, and calcium phosphate deposition ability were evaluated on textiles at different time intervals under a confocal laser scanning microscope. The osteoblast viability ranged from 92% to 98%, and cell proliferation was higher in PGA-RGD than control PGA (uncoated). In addition, the osteoblast calcium phosphate deposition was significantly greater on PGA-RGD in osteogenic inductor medium (OIM) in contrast to controls without inducing factors. The PGA-RGD fibers supported proliferation and viability of osteoblast and stimulated bone osteogenesis and mineralization. These results support the adoption of this 3D polymeric textile as a scaffold for bone tissue engineering.

scholarly journals Electrospun oriented gelatin-hydroxyapatite fiber scaffolds for bone tissue engineering

2017 ◽  
Vol 105 (7) ◽  
pp. 1911-1926 ◽  
Author(s):  
Ali A. Salifu ◽  
Constantina Lekakou ◽  
Fatima H. Labeed
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Fiber Scaffolds

scholarly journals Composite nanoclay-hydroxyapatite-polymer fiber scaffolds for bone tissue engineering manufactured using pressurized gyration

2021 ◽  
Vol 202 ◽  
pp. 108598
Author(s):  
Krishna Kundu ◽  
Ayda Afshar ◽  
Dinesh R. Katti ◽  
Mohan Edirisinghe ◽  
Kalpana S. Katti
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Polymer Fiber ◽  
Fiber Scaffolds

HYDROXYAPATITE, ALGINATE, AND CHITOSAN FOR BONE SCAFFOLDS: SPECTROSCOPY STUDY

2016 ◽  
Vol 19 (2) ◽  
pp. 93-100
Author(s):  
Lalita El Milla
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Functional Groups ◽  
Bone Growth ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Tissue Engineering Scaffolds ◽  
Hydroxyapatite Powder ◽  
Materials Used

Scaffolds is three dimensional structure that serves as a framework for bone growth. Natural materials are often used in synthesis of bone tissue engineering scaffolds with respect to compliance with the content of the human body. Among the materials used to make scafffold was hydroxyapatite, alginate and chitosan. Hydroxyapatite powder obtained by mixing phosphoric acid and calcium hydroxide, alginate powders extracted from brown algae and chitosan powder acetylated from crab. The purpose of this study was to examine the functional groups of hydroxyapatite, alginate and chitosan. The method used in this study was laboratory experimental using Fourier Transform Infrared (FTIR) spectroscopy for hydroxyapatite, alginate and chitosan powders. The results indicated the presence of functional groups PO43-, O-H and CO32- in hydroxyapatite. In alginate there were O-H, C=O, COOH and C-O-C functional groups, whereas in chitosan there were O-H, N-H, C=O, C-N, and C-O-C. It was concluded that the third material containing functional groups as found in humans that correspond to the scaffolds material in bone tissue engineering.

PLGA+HA/βTCP scaffold incorporating simvastatin: a promising biomaterial for bone tissue engineering

2020 ◽  
Author(s):  
Mariane Beatriz Sordi ◽  
Ariadne Cristiane Cabral da Cruz ◽  
Águedo Aragones ◽  
Mabel Mariela Rodríguez Cordeiro ◽  
Ricardo de Souza Magini
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Thermal Analyses ◽  
Chemical Properties ◽  
Biological Properties ◽  
Scanning Calorimetry ◽  
Evaporation Technique ◽  
Porosity Analysis ◽  
Biphasic Ceramic

The aim of this study was to synthesize, characterize, and evaluate degradation and biocompatibility of poly(lactic-co-glycolic acid) + hydroxyapatite / β-tricalcium phosphate (PLGA+HA/βTCP) scaffolds incorporating simvastatin (SIM) to verify if this biomaterial might be promising for bone tissue engineering. Samples were obtained by the solvent evaporation technique. Biphasic ceramic particles (70% HA, 30% βTCP) were added to PLGA in a ratio of 1:1. Samples with SIM received 1% (m:m) of this medication. Scaffolds were synthesized in a cylindric-shape and sterilized by ethylene oxide. For degradation analysis, samples were immersed in PBS at 37 °C under constant stirring for 7, 14, 21, and 28 days. Non-degraded samples were taken as reference. Mass variation, scanning electron microscopy, porosity analysis, Fourier transform infrared spectroscopy, differential scanning calorimetry, and thermogravimetry were performed to evaluate physico-chemical properties. Wettability and cytotoxicity tests were conducted to evaluate the biocompatibility. Microscopic images revealed the presence of macro, meso, and micropores in the polymer structure with HA/βTCP particles homogeneously dispersed. Chemical and thermal analyses presented very similar results for both PLGA+HA/βTCP and PLGA+HA/βTCP+SIM. The incorporation of simvastatin improved the hydrophilicity of scaffolds. Additionally, PLGA+HA/βTCP and PLGA+HA/βTCP+SIM scaffolds were biocompatible for osteoblasts and mesenchymal stem cells. In summary, PLGA+HA/βTCP scaffolds incorporating simvastatin presented adequate structural, chemical, thermal, and biological properties for bone tissue engineering.

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