scholarly journals Bakteri Endofit asal Tanaman Tembakau sebagai Agens Pengendali Meloidogyne spp.

2019 ◽  
Vol 14 (6) ◽  
pp. 215 ◽  
Author(s):  
Isnainy dinul Mursyalatiyus ◽  
Abdul Munif ◽  
Abdjad Asih Nawangsih
Keyword(s):  
Biological Control ◽  
Endophytic Bacteria ◽  
Biological Control Agent ◽  
Tobacco Plant ◽  
Control Agent ◽  
Root Knot Nematode ◽  
Tobacco Plants ◽  
Physiological Characterization ◽  
Protease Enzyme ◽  
Meloidogyne Spp

Endophytic Bacteria from Tobacco Plant as Biocontrol Agent of  Meloidogyne spp.Soilborne disease on tobacco plants caused by fungal and bacterial infection in association with root-knot nematode (Meloidogyne spp.) may cause significant yield loss.  Endophytic bacteria have been recognized as biological control agent for Meloidogyne spp. as well as  plant promoting growth agent. Research was conducted to evaluate endophytic bacteria isolated from tobacco plants as biological control agent for Meloidogyne spp. infecting tobacco. A total of 215 isolates of endophytic bacteria were isolated from root of two tobacco varieties, Kemloko and Prancak 95.  Biosafety screening showed that 80 isolates (37%) and 7 isolates (8%) gave negative reaction on hypersensitivity test and hemolysis test, respectively.  Seven isolates i.e. TPT3.10, TPT2.1, TK3n8, TK2t21, TK2n8, TK3n1 and TK2t11 were able to promote plant growth and increase the mortality of juvenile Meloidogyne spp. Physiological characterization of endophytic bacteria showed that most of the isolates were able to produce protease enzyme, phosphate, nitrogen and HCN. The same isolates were also able to suppress the number of galls from 80.09% up to 93.82%. Two isolates, TPT3.10 and TK2n8, are considered having the best suppression on root gall formation.

Effect of nematophagous fungi on reproduction of Meloidogyne enterolobii on guava (Psidium guajava) plants

Nematology ◽  
2011 ◽  
Vol 13 (6) ◽  
pp. 721-728 ◽  
Author(s):  
Myrian Tigano ◽  
Karla Fernanda Ayres de Souza Silva ◽  
Irene Martins ◽  
Regina M.D. Gomes Carneiro ◽  
Leopoldo Hidalgo-Díaz ◽  
...  
Keyword(s):  
Biological Control ◽  
Biological Control Agent ◽  
Integrated Management ◽  
Pathogenic Fungi ◽  
Psidium Guajava ◽  
Control Agent ◽  
Root Knot Nematode ◽  
Plastic Bags ◽  
Meloidogyne Enterolobii ◽  
Meloidogyne Spp

AbstractThe root-knot nematode, Meloidogyne enterolobii, is a major disease of guava, Psidium guajava, in Brazil and other countries. Egg-pathogenic fungi are considered potential biological control agents of root-knot nematodes and are associated with suppression of Meloidogyne spp. Glasshouse experiments were conducted in order to evaluate the effect of the fungi Paecilomyces lilacinus and Pochonia chlamydosporia on a population of M. enterolobii growing on guava plants. Guava seedlings of about 15-20 cm growing in plastic bags were inoculated with 10 000 eggs of M. enterolobii plant−1. Two months later, three isolates of P. lilacinus and one isolate of P. chlamydosporia were inoculated in the infested plants. The effect of the treatments was evaluated 6 months later. Although plant infection by nematodes was not attenuated, the number of eggs (g roots)−1 fell significantly. The number of egg masses infected with the fungi was inversely correlated with the number of eggs found in the roots. The most effective result (61.5% of control) was obtained with the isolate CG1003 of P. chlamydoporia, which was originally isolated from eggs of M. enterolobii in Brazil, followed by P. lilacinus (CG959 and CG1038) with about 40% of control. These fungi showed the ability to colonise healthy guava roots in glasshouse experiments. These results suggest that P. chlamydosporia can be selected as a potential biological control agent to be employed with other strategies in integrated management to control M. enterolobii on guava.

Development of Pasteuria penetrans on different field populations of Meloidogyne spp. from Pakistan

Nematology ◽  
2019 ◽  
Vol 21 (10) ◽  
pp. 1003-1010
Author(s):  
Muhammad Kamran ◽  
Nazir Javed ◽  
Huma Abbas ◽  
Sajid Aleem Khan ◽  
Ihsan Ullah ◽  
...  
Keyword(s):  
Biological Control ◽  
Root System ◽  
Biological Control Agent ◽  
Control Agent ◽  
Pasteuria Penetrans ◽  
Successful Development ◽  
Nematode Reproduction ◽  
Tomato Roots ◽  
Differential Infectivity ◽  
Meloidogyne Spp

Summary Pasteuria penetrans is a hyperparasite with potential as a biological control agent of root-knot nematodes, Meloidogyne spp. In this study two exotic Pasteuria isolates (PP-3 and PP-J) were developed on different Meloidogyne spp. collected from different districts of Punjab, Pakistan. The development of PP-3 and PP-J on different field populations of Meloidogyne spp. showed differential infectivity in terms of nematode reproduction variables. The development of PP-3 and PP-J was maximum on tomato as compared to cucumber. It was probably due to more invasion sites for nematodes present in the tomato roots. However, a bigger root system has not always been a guarantee for development of Pasteuria on Meloidogyne spp. For successful development of Pasteuria, the key parameters are the percentage of infected females, number of endospores (female)−1 or endospores (mg root powder)−1. The results of these parameters are greater in tomato as compared to cucumber.

scholarly journals Isolation and effect of Trichoderma citrinoviride Snef1910 for the biological control of root-knot nematode, Meloidogyne incognita

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Haiyan Fan ◽  
Meiling Yao ◽  
Haiming Wang ◽  
Di Zhao ◽  
Xiaofeng Zhu ◽  
...  
Keyword(s):  
Biological Control ◽  
Meloidogyne Incognita ◽  
Plant Biomass ◽  
Biological Control Agent ◽  
Control Agent ◽  
Vegetable Crops ◽  
Root Knot Nematode ◽  
Egg Hatching ◽  
Tomato Plants ◽  
Trichoderma Citrinoviride

Abstract Background Root-knot nematode is one of the most significant diseases of vegetable crops in the world. Biological control with microbial antagonists has been emerged as a promising and eco-friendly treatment to control pathogens. The aim of this study was to screen and identify novel biocontrol agents against root-knot nematode, Meloidogyne incognita. Results A total of 890 fungal isolates were obtained from rhizosphere soil of different crops and screened by nematicidal activity assays. Snef1910 strain showed high virulence against second stage juveniles (J2s) of M. incognita and identified as Trichoderma citrinoviride by morphology analysis and biomolecular assay. Furthermore, T. citrinoviride Snef1910 significantly inhibited egg hatching with the hatching inhibition percentages of 90.27, 77.50, and 67.06% at 48, 72, and 96 h after the treatment, respectively. The results of pot experiment showed that the metabolites of T. citrinoviride Snef1910 significantly decreased the number of root galls, J2s, and nematode egg masses and J2s population density in soil and significantly promoted the growth of tomato plants. In the field experiment, the biocontrol application showed that the control efficacy of T. citrinoviride Snef1910 against root-knot nematode was more than 50%. Meanwhile, T. citrinoviride Snef1910 increased the tomato plant biomass. Conclusions T. citrinoviride strain Snef1910 could be used as a potential biological control agent against root-knot nematode, M. incognita.

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