scholarly journals Growth performance, fast muscle development and chemical composition of juvenile lumpfish (Cyclopterus lumpus) fed diets incorporating soy and pea protein concentrates

2020 ◽  
Vol 17 ◽  
pp. 100352
Author(s):  
Florence Perera Willora ◽  
Nimalan Nadanasabesan ◽  
Helene Rønquist Knutsen ◽  
Cui Liu ◽  
Mette Sørensen ◽  
...  
2020 ◽  
Author(s):  
Gist H. Farr ◽  
Bingsi Li ◽  
Maurizio Risolino ◽  
Nathan M. Johnson ◽  
Zizhen Yao ◽  
...  

SummaryVertebrate skeletal muscles are composed of both slow-twitch and fast-twitch fiber types. How the differentiation of distinct fiber types is activated during embryogenesis is not well characterized. Skeletal muscle differentiation is initiated by the activity of the myogenic basic helix-loop-helix (bHLH) transcription factors Myf5, Myod1, Myf6, and Myog. Myod1 functions as a muscle master regulatory factor and directly activates muscle differentiation genes, including those specific to both slow and fast muscle fibers. Our previous studies showed that Pbx TALE-class homeodomain proteins bind with Myod1 on the promoter of the zebrafish fast muscle gene mylpfa and are required for proper activation of mylpfa expression and the fast-twitch muscle-specific differentiation program in zebrafish embryos. Pbx proteins have also been shown to bind regulatory regions of muscle differentiation genes in mammalian muscle cells in culture. Here, we use new zebrafish mutant strains to confirm the essential roles of zebrafish Pbx factors in embryonic fast muscle differentiation. Furthermore, we examine the requirements for Pbx genes in mouse embryonic skeletal muscle differentiation, an area that has not been investigated in the mammalian embryo. Removing Pbx1 function from skeletal muscle in Myf5Cre/+;Pbx1fl/fl mouse embryos has minor effects on embryonic muscle development. However, concomitantly deleting Pbx2 function in Myf5Cre/+;Pbx1fl/fl;Pbx2-/- mouse embryos causes delayed activation and reduced expression of fast muscle differentiation genes. In the mouse, Pbx1/Pbx2-dependent fast muscle genes closely match those that have been previously shown to be dependent on murine Six1 and Six4. This work establishes evolutionarily conserved requirements for Pbx factors in embryonic fast muscle differentiation. Our studies are revealing how Pbx homeodomain proteins help direct specific cellular differentiation pathways.


2021 ◽  
Vol 48 (2) ◽  
pp. 148-161
Author(s):  
R. S. Onabanjo ◽  
G. S. Ojewola ◽  
D. N. Onunkwo ◽  
O. O. Adedokun ◽  
E. U. Ewa ◽  
...  

The growing concern about the cost of feed ingredients, use of least cost feed formulation and maintenance of a reliable nutritional requirement for broiler birds irrespective of the season is almost compelling to look for alternatives. Thus, this experiment was conducted to evaluate the performance of broiler chickens fed rice milling waste (RMW) as a replacement for maize. Chemical composition of rice milling waste was determined, while eleven experimental diets each were formulated to contain RMW as replacement for maize at 0%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% and 100%. Three hundred (300), 7-day-old chicks were allocated to the 11 dietary treatments each having 3 replicate pens with 10 birds per pen in a Completely Randomized Design (CRD). Feed and water were provided ad libitum for 6 weeks. Growth performance, carcass yield, organ proportions and economics of production were monitored. Data were subjected to ANOVA at P<0.05. The result from the chemical composition showed that rice milling waste has 89.84% dry matter, 10.80% crude protein and 24.09% crude fibre, while the gross energy was 3789 kcal/g, this showed that RMW is suitable as feed ingredient and as well would not hinder absorption and nutrient utilization. The result of the growth performance showed that broiler chickens fed 10% - 40% RMW had greater average daily weight gain (55.33g- 47.97g), reached a greater slaughter and carcass weight (1500g – 1650g) and were more feed efficient than broiler chicken of the other treatments (P<0.05 for each). In addition, from an economical point of view birds fed 10 - 40% were superior to the other treatments where the cost/kg weight gained (N292.21K – N315.96K) were lower than the remaining treatment groups. Thus, the inclusion of RMW in diet of broiler chickens as a replacement for maize improved nutrient utilization and economic value. It can therefore be recommended that RMW can be used to replace maize between 10% -40% in the diets of broiler chickens.     La préoccupation croissante au sujet du coût des ingrédients alimentaires, de l'utilisation de la formulation d'aliments pour animaux les moins coûteux et du maintien d'une exigence nutritionnelle fiable pour les oiseaux de gril, quelle que soit la saison, est presque convaincante pour chercher d'autres solutions. Ainsi, cette expérience a été menée pour évaluer la performance des poulets à griller nourris aux déchets de fraisage du riz (le 'RMW') comme remplacement du maïs. La composition chimique des déchets de fraisage du riz a été déterminée, tandis que onze régimes expérimentaux ont été formulés pour contenir le 'RMW' comme remplacement pour le maïs à 0%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% et 100%. Trois cents (300), poussins de 7 jours ont été alloués aux 11 traitements diététiques ayant chacun 3 stylos de répétition avec 10 oiseaux par stylo dans un design complètement randomisé (CRD). L'alimentation et l'eau ont été fournies ad libitum pendant 6 semaines. Les performances de croissance, le rendement de la carcasse, les proportions d'organes et l'économie de la production ont été surveillés. Les données ont été soumises à ANOVA (P<0.05) Le résultat de la composition chimique a montré que les déchets de fraisage du riz ont 89,84% de matière sèche, 10,80% de protéines brutes et 24,09% de fibres brutes, tandis que l'énergie brute était de 3789 kcal/g, ce qui a montré que le 'RMW' est approprié comme ingrédient d'alimentation et ainsi n'entraverait pas l'absorption et l'utilisation des nutriments. Le résultat de la performance de croissance a montré que les poulets a griller nourris 10% - 40% RMW avaient un gain de poids quotidien moyen plus élevé (55.33g- 47.97g), ont atteint un plus grand poids d'abattage et de carcasse (1500g - 1650g) et étaient plus économes en aliments pour des animaux que le poulet de gril des autres traitements (P<0.05 pour chacun). En outre, d'un point de vue économique, les oiseaux nourris de 10 à 40 % étaient supérieurs aux autres traitements où le coût/kg de poids gagné (N292.21 K – N315.96 K) était inférieur à celui des autres groupes de traitement. Ainsi, l'inclusion du RMW dans l'alimentation des poulets de poulet a griller comme remplacement du maïs a amélioré l'utilisation des nutriments et la valeur économique. Il peut donc être recommandé que le 'RMW' peut être utilisé pour remplacer le maïs entre 10% -40% dans les régimes des poulets à griller.


Author(s):  
Ana Paula Hanke de Oliveira ◽  
Michele Harumi Omura ◽  
Éverton de Almeida Alves Barbosa ◽  
Gustavo Costa Bressan ◽  
Érica Nascif Rufino Vieira ◽  
...  

1974 ◽  
Vol 24 (1-2) ◽  
pp. 71-84 ◽  
Author(s):  
A. M. Youssef ◽  
Mohamed M. El-Fouly ◽  
F. K. El-Baz

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