Protein content and amino acid composition of the live feed rotifer (Brachionus plicatilis): With emphasis on the water soluble fraction

The purpose of this study was to analyse the amino acid composition of otolith matrix protein, estimate the proportion of the water-soluble protein fraction, and analyse the effect of matrix composition on otolith visual appearance. Juvenile Atlantic cod (Gadus morhua) were reared under constant temperature and feeding conditions and sampled at the beginning and the end of the experiment. The amino acid composition was dominated by asparagine, glutamic acid, leucine, serine, and proline. A change in amino acid composition was observed with increasing temperature and time, caused by changing proportions of the water-soluble and -insoluble protein fractions. Feeding level had no effect. The relative content of water-soluble protein was linearly related to fish dry weight and temperature. Otolith opacity, defined as the percentage of incident light absorbed by an otolith section, did not differ significantly between experimental treatments. The soluble protein fraction had a positive, albeit insignificant, correlation with opacity. Using opacity and otolith volume, deposited total otolith protein content was estimated with an R2 of 0.91, where otolith volume alone explained 83% of the observed variation.

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Proteins in the Enamel Fluid of Immature Porcine Teeth

Journal of Dental Research ◽

10.1177/00220345870660120501 ◽

1987 ◽

Vol 66 (12) ◽

pp. 1721-1726 ◽

Cited By ~ 31

Author(s):

T. Aoba ◽

T. Tanabe ◽

E.C. Moreno

Keyword(s):

Amino Acid ◽

Amino Acid Composition ◽

Protein Content ◽

Acid Composition ◽

Permanent Teeth ◽

Major Protein ◽

Protein Species ◽

Terminal Sequence ◽

Molecular Weights ◽

Sds Electrophoresis

The fluid was separated from the immature soft enamel of porcine permanent teeth in the secretory stage according to procedures reported previously (Aoba and Moreno, 1987). The protein content of the fluid was about 2.8% w/v; its amino-acid composition was characterized by high contents of Pro, Glx, Leu, and His, showing composition similar to that of the 20 kilo-dalton (kd) amelogenin or its C-terminal segments. The two major protein species in the fluid had apparent molecular weights of 13 kd and 11 kd, as determined by SDS electrophoresis; the N-terminal residue of the former was Leu, while that of the latter was Ala. The C-terminal sequence of both of them was -Met-Phe-Ser. By comparison with the published sequence of 20-kd porcine amelogenin, it is concluded that the main fluid constituents were derived by cleavages of N-terminal segments from the 20-kd amelogenin.

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NATURE OF PROTEINS IN TRITICALE AND ITS PARENTAL SPECIES: I. SOLUBILITY CHARACTERISTICS AND AMINO ACID COMPOSITION OF ENDOSPERM PROTEINS

Canadian Journal of Plant Science ◽

10.4141/cjps70-002 ◽

1970 ◽

Vol 50 (1) ◽

pp. 9-14 ◽

Cited By ~ 88

Author(s):

C. H. CHEN ◽

W. BUSHUK

Keyword(s):

Amino Acid ◽

Durum Wheat ◽

Amino Acid Composition ◽

Acid Composition ◽

Spring Wheat ◽

Water Soluble ◽

The Other ◽

Hard Red Spring Wheat ◽

Endosperm Proteins ◽

Spring Wheat Cultivar

Solubility characteristics and amino acid composition of the endosperm proteins of one line of Triticale, its durum wheat and rye parent cultivars, and one cultivar of hard red spring wheat were compared. Quantitative distribution of the soluble protein fractions and amino acid compositions showed that the proteins of Triticale are intermediate in these properties between analogous properties of the proteins of its durum wheat and rye parents. The major differences between the hard red spring wheat and the other three species were its lower content of water-soluble proteins and higher content of insoluble or gluten proteins. This appears lo be the main reason for the superior breadmaking quality of the hard red spring wheat cultivar compared with the other species used in this study.

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Protein content and amino acid composition of eggs and endotrophic larvae from wild and captive fed sea urchinParacentrotus lividus(Echinodermata: Echinoidea)

Aquaculture Research ◽

10.1111/are.12475 ◽

2014 ◽

Vol 47 (1) ◽

pp. 114-127 ◽

Cited By ~ 1

Author(s):

João Gago ◽

Tiago Martins ◽

Orlando J Luís

Keyword(s):

Amino Acid ◽

Amino Acid Composition ◽

Protein Content ◽

Acid Composition

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INFLUENCE OF DRYING METHODS ON PROTEIN CONTENT AND AMINO ACID COMPOSITION OF THREE FORAGE LEGUMES

Canadian Journal of Plant Science ◽

10.4141/cjps79-110 ◽

1979 ◽

Vol 59 (3) ◽

pp. 707-712 ◽

Cited By ~ 2

Author(s):

M. S. KALDY ◽

M. R. HANNA ◽

S. SMOLIAK

Keyword(s):

Amino Acid ◽

Amino Acid Composition ◽

Protein Content ◽

Acid Composition ◽

Dry Matter ◽

Protein Quality ◽

Microwave Drying ◽

Drying Methods ◽

Dry Matter Content ◽

Forced Draft

Forage samples of alfalfa (Medicago sativa L. ’Beaver’), sainfoin (Onobrychis viciaefolia Scop. ’Melrose’), and cicer milkvetch (Astragalus cicer L. ’Oxley’) were dried either in a forced-draft forage dryer, drying oven, or a microwave oven. The dry matter content, protein content, and amino acid composition were determined but the air-drying methods resulted in significant losses of dry matter compared with microwave drying, suggesting that the latter method is preferable for dry-matter determinations. Interactions between species and drying method were significant for some of the amino acid contents, but not for the limiting amino acids — methionine, isoleucine, and valine. Since protein scores, reflecting protein quality, and protein content of the three legumes were not affected by drying methods, any one of the drying methods would be satisfactory. However, large numbers of forage samples can be handled most conveniently in a forced-draft dryer.

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