scholarly journals Simultaneous quantification of nimesulide, phenylpropanolamine, caffeine and chlorpheniramine in rat plasma by RP–HPLC/PDA method and application to pharmacokinetic studies in healthy rat subjects

2019 ◽  
Vol 12 (8) ◽  
pp. 2320-2327 ◽  
Author(s):  
Thippani Ramesh ◽  
Pothuraju Nageswara Rao ◽  
Ramisetti Nageswara Rao
Keyword(s):  
Rat Plasma ◽  
Pharmacokinetic Studies ◽  
Simultaneous Quantification ◽  
Rp Hplc

scholarly journals LC-MS/MS Method for Simultaneous Quantification of Dexmedetomidine, Dezocine, and Midazolam in Rat Plasma and Its Application to Their Pharmacokinetic Study

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Wenjuan Cui ◽  
Qin Liu ◽  
Shan Xiong ◽  
Lujun Qiao
Keyword(s):  
Storage Conditions ◽  
Rat Plasma ◽  
Selected Reaction Monitoring ◽  
Pharmacokinetic Studies ◽  
Reaction Monitoring ◽  
Simultaneous Quantification ◽  
Ionization Mode ◽  
The Stability ◽  
Lower Limits ◽  
Interday Precision

A simple, sensitive, and accurate LC-MS/MS method was established and validated for the simultaneous quantification of dexmedetomidine, dezocine, and midazolam in rat plasma. Chromatographic separation was achieved on a C18 column (50 mm × 2.1 mm, 3 µm) using a mobile phase composed of water (containing 0.1% formic acid) and acetonitrile. The lower limits of quantification were 0.1, 0.1, and 0.2 ng/mL for dexmedetomidine, dezocine, and midazolam in rat plasma, respectively. The analytes were determined with selected reaction monitoring under positive ionization mode. The intra- and interday precision and accuracy were all within acceptable limits during the entire validation, and the stability of analytes was acceptable under various storage conditions. The validated method was successfully applied in pharmacokinetic studies of dexmedetomidine, dezocine, and midazolam following intravenous injection.

scholarly journals A simple sensitive UFLC-MS/MS method for the simultaneous quantification of artesunate, dihydroartemisinin and quercetin in rat plasma and its application to pharmacokinetic studies

RSC Advances ◽  
2019 ◽  
Vol 9 (71) ◽  
pp. 41794-41802
Author(s):  
Nethravathi Puttappa ◽  
Karthik Yamjala ◽  
Narenderan S. T. ◽  
Suresh Kumar Raman ◽  
Gowthamarajan Kuppusamy ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Active Metabolite ◽  
Rat Plasma ◽  
Simultaneous Estimation ◽  
Pharmacokinetic Studies ◽  
Tandem Mass ◽  
Simultaneous Quantification ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

An ultrafast liquid chromatography-tandem mass spectrometry (UFLC-MS/MS) method was developed for the simultaneous estimation of artesunate (ART), dihydroartemisinin (DHA, an active metabolite of ART) and quercetin (QRT) in rat plasma.

Development of validated RP HPLC method with fluorescence detection for simultaneous quantification of sacubitril and valsartan from rat plasma

2018 ◽  
Vol 41 (5) ◽  
pp. 246-252 ◽  
Author(s):  
Mahesh Attimarad ◽  
Sree Harsha Nagaraja ◽  
Anroop Balachandran Nair ◽  
Bandar Essa Aldhubaib ◽  
Venugopala Narayanaswamy Katharigatta
Keyword(s):  
Fluorescence Detection ◽  
Rat Plasma ◽  
Hplc Method ◽  
Simultaneous Quantification ◽  
Rp Hplc

scholarly journals Development and validation of RP-HPLC method for sildenafil citrate in rat plasma-application to pharmacokinetic studies

2013 ◽  
Vol 21 (3) ◽  
pp. 317-321 ◽  
Author(s):  
A.S. Tripathi ◽  
I. Sheikh ◽  
A.P. Dewani ◽  
P.G. Shelke ◽  
R.L. Bakal ◽  
...  
Keyword(s):  
Rat Plasma ◽  
Hplc Method ◽  
Sildenafil Citrate ◽  
Pharmacokinetic Studies ◽  
Rp Hplc ◽  
Development And Validation

Analytical and bioanalytical HPLC method for simultaneous estimation of 5-fluorouracil and sonidegib

Bioanalysis ◽  
2021 ◽  
Author(s):  
Bharath S Padya ◽  
Aswathi R Hegde ◽  
Sadhana P Mutalik ◽  
Swati Biswas ◽  
Srinivas Mutalik
Keyword(s):  
Analytical Procedure ◽  
Gradient Elution ◽  
Rat Plasma ◽  
Hplc Method ◽  
Simultaneous Estimation ◽  
Simultaneous Quantification ◽  
Liquid Liquid Extraction ◽  
Rp Hplc ◽  
Dose Oral ◽  
Methods Analytical

Aim: To develop a new sensitive RP-HPLC method for simultaneous estimation of 5-fluorouracil (5-FU) and sonidegib (SDG). Materials & methods: Analytical and bioanalytical methods for simultaneous quantification of 5-FU and SDG in bulk, nanoformulations and in rat plasma were developed and validated using a gradient elution technique. Results: Separation of the analytes was effected on a Luna® C18 LC column using a mobile mixture comprising acetonitrile and acidified water. 5-FU and SDG were extracted from plasma matrix using liquid–liquid extraction. The applicability of the method was verified through single-dose oral pharmacokinetic study in Wistar rats. Conclusion: The developed methods allow a specific, sensitive and steady analytical procedure for the simultaneous estimation of 5-FU and SDG in nanoformulations and biological matrix.

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