Simultaneous quantification of ligustilide, dl ‐3 ‐n‐ butylphthalide and senkyunolide A in rat plasma by GC–MS and its application to comparative pharmacokinetic studies of Rhizoma Chuanxiong extract alone and Baizhi Chuanxiong Decoction

2019 ◽  
Vol 33 (10) ◽  
Author(s):  
Qinhui Wang ◽  
Tao Yan ◽  
Wei Jiang ◽  
Na Hu ◽  
Song Zhang ◽  
...  
Keyword(s):  
Rat Plasma ◽  
Pharmacokinetic Studies ◽  
Comparative Pharmacokinetic ◽  
Rhizoma Chuanxiong ◽  
Simultaneous Quantification

scholarly journals LC-MS/MS Method for Simultaneous Quantification of Dexmedetomidine, Dezocine, and Midazolam in Rat Plasma and Its Application to Their Pharmacokinetic Study

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Wenjuan Cui ◽  
Qin Liu ◽  
Shan Xiong ◽  
Lujun Qiao
Keyword(s):  
Storage Conditions ◽  
Rat Plasma ◽  
Selected Reaction Monitoring ◽  
Pharmacokinetic Studies ◽  
Reaction Monitoring ◽  
Simultaneous Quantification ◽  
Ionization Mode ◽  
The Stability ◽  
Lower Limits ◽  
Interday Precision

A simple, sensitive, and accurate LC-MS/MS method was established and validated for the simultaneous quantification of dexmedetomidine, dezocine, and midazolam in rat plasma. Chromatographic separation was achieved on a C18 column (50 mm × 2.1 mm, 3 µm) using a mobile phase composed of water (containing 0.1% formic acid) and acetonitrile. The lower limits of quantification were 0.1, 0.1, and 0.2 ng/mL for dexmedetomidine, dezocine, and midazolam in rat plasma, respectively. The analytes were determined with selected reaction monitoring under positive ionization mode. The intra- and interday precision and accuracy were all within acceptable limits during the entire validation, and the stability of analytes was acceptable under various storage conditions. The validated method was successfully applied in pharmacokinetic studies of dexmedetomidine, dezocine, and midazolam following intravenous injection.

scholarly journals A simple sensitive UFLC-MS/MS method for the simultaneous quantification of artesunate, dihydroartemisinin and quercetin in rat plasma and its application to pharmacokinetic studies

RSC Advances ◽  
2019 ◽  
Vol 9 (71) ◽  
pp. 41794-41802
Author(s):  
Nethravathi Puttappa ◽  
Karthik Yamjala ◽  
Narenderan S. T. ◽  
Suresh Kumar Raman ◽  
Gowthamarajan Kuppusamy ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Active Metabolite ◽  
Rat Plasma ◽  
Simultaneous Estimation ◽  
Pharmacokinetic Studies ◽  
Tandem Mass ◽  
Simultaneous Quantification ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

An ultrafast liquid chromatography-tandem mass spectrometry (UFLC-MS/MS) method was developed for the simultaneous estimation of artesunate (ART), dihydroartemisinin (DHA, an active metabolite of ART) and quercetin (QRT) in rat plasma.

scholarly journals A Simple HPLC Method for the Quantitative Determination of Silybin in Rat Plasma: Application to a Comparative Pharmacokinetic Study on Commercial Silymarin Products

Molecules ◽  
2019 ◽  
Vol 24 (11) ◽  
pp. 2180 ◽  
Author(s):  
Eun-Sol Ha ◽  
Dong-Gyun Han ◽  
Seong-Wook Seo ◽  
Ji-Min Kim ◽  
Seon-Kwang Lee ◽  
...  
Keyword(s):  
Pharmacokinetic Study ◽  
Gradient Elution ◽  
Rat Plasma ◽  
Hplc Method ◽  
Pharmacokinetic Studies ◽  
Active Constituent ◽  
Promising Alternative ◽  
Comparative Pharmacokinetic ◽  
Bioanalytical Method

Silybin (SBN) is a major active constituent of silymarin, a mixture of flavonoids found in fruits and seeds of milk thistle. The aim of this study was to describe a simple bioanalytical method for quantifying SBN in rat plasma. A simple protein deproteinization procedure with acetonitrile (ACN) was employed for plasma sample preparation. A reversed column and gradient elution of a mobile phase (mixture of phosphate buffer (pH 5.0) and ACN) were used for chromatographic separation. The selectivity, linearity (50–5000 ng/mL), precision, accuracy, recovery, matrix effect, and stability for this method were validated as per the current Food and Drug Administration (FDA) guidelines. Our method for SBN was applied to a comparative pharmacokinetic study on four different commercial silymarin products. This in vivo rat study demonstrated that product #4 significantly enhanced the relative oral bioavailability of SBN, as compared to product #1–3. Therefore, the bioanalytical method proposed herein could serve as a promising alternative for preclinical pharmacokinetic studies on silymarin products and, by extension, clinical use after partial modification and validation.

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