Multiplexing the dynamic vibration absorber (DVA) has tried for improving the vibration absorption efficiency of the dynamic vibration absorber, and because there are several tuning vibration frequencies in the multi-mass dynamic vibration absorber system, the vibration absorption efficiency is more excellent than a conventional single-mass dynamic vibration absorber when they are in the same total mass ratio. In this paper, a two-series-mass dynamic vibration absorber was proposed, optimal parameters of the two-series-mass dynamic vibration absorber have been calculated and vibration absorption is analyzed with the simulation and experiment. The results show that the two-series-mss dynamic vibration absorber can improve vibration absorption, in the same total mass ratio, by about 20% above that of a conventional single-mass dynamic vibration absorber. Based on the above optimal results, the two-series-mass dynamic vibration was used for vibration control on high building, and the practical measure result shows its effectiveness.