scholarly journals Evaluating the fluorescent tracer 800CW-TATE for molecular fluorescence guided surgery in meningiomas

2021 ◽  
Vol 1 ◽  
pp. 100526
Author(s):  
B.M. Dijkstra ◽  
M. de Jong ◽  
M.C.M. Stroet ◽  
F. Andreae ◽  
S.E. Dulfer ◽  
...  
Keyword(s):  
Fluorescent Tracer ◽  
Guided Surgery ◽  
Fluorescence Guided Surgery ◽  
Molecular Fluorescence

scholarly journals Correction to: Evaluation of Ac-Lys0(IRDye800CW)Tyr3-octreotate as a novel tracer for SSTR2-targeted molecular fluorescence guided surgery in meningioma

2021 ◽  
Author(s):  
Bianca M. Dijkstra ◽  
Marion de Jong ◽  
Marcus C. M. Stroet ◽  
Fritz Andreae ◽  
Sebastiaan E. Dulfer ◽  
...  
Keyword(s):  
Guided Surgery ◽  
Fluorescence Guided Surgery ◽  
Molecular Fluorescence

A correction to this paper has been published: https://doi.org/10.1007/s11060-021-03769-9

scholarly journals Evaluation of Ac-Lys0(IRDye800CW)Tyr3-octreotate as a novel tracer for SSTR2-targeted molecular fluorescence guided surgery in meningioma

2021 ◽  
Author(s):  
Bianca M. Dijkstra ◽  
Marion de Jong ◽  
Marcus C. M. Stroet ◽  
Fritz Andreae ◽  
Sebastiaan E. Dulfer ◽  
...  
Keyword(s):  
Binding Affinity ◽  
Ex Vivo ◽  
Specific Binding ◽  
Somatostatin Receptor ◽  
Receptor Subtype ◽  
Tumor Uptake ◽  
In Vivo Models ◽  
Guided Surgery ◽  
Fluorescence Guided Surgery ◽  
Molecular Fluorescence

Abstract Purpose Meningioma recurrence rates can be reduced by optimizing surgical resection with the use of intraoperative molecular fluorescence guided surgery (MFGS). We evaluated the potential of the fluorescent tracer 800CW-TATE for MFGS using in vitro and in vivo models. It targets somatostatin receptor subtype 2 (SSTR2), which is overexpressed in all meningiomas. Methods Binding affinity of 800CW-TATE was evaluated using [177Lu] Lu-DOTA-Tyr3-octreotate displacement assays. Tumor uptake was determined by injecting 800CW-TATE in (SSTR2-positive) NCI-H69 or (SSTR2-negative) CH-157MN xenograft bearing mice and FMT2500 imaging. SSTR2-specific binding was measured by comparing tumor uptake in NCI-H69 and CH-157MN xenografts, blocking experiments and non-targeted IRDye800CW-carboxylate binding. Tracer distribution was analyzed ex vivo, and the tumor-to-background ratio (TBR) was calculated. SSTR2 expression was determined by immunohistochemistry (IHC). Lastly, 800CW-TATE was incubated on frozen and fresh meningioma specimens and analyzed by microscopy. Results 800CW-TATE binding affinity assays showed an IC50 value of 72 nM. NCI-H69 xenografted mice showed a TBR of 21.1. 800CW-TATE detection was reduced after co-administration of non-fluorescent DOTA-Tyr3-octreotate or administration of IRDye800CW. CH-157MN had no tumor specific tracer staining due to absence of SSTR2 expression, thereby serving as a negative control. The tracer bound specifically to SSTR2-positive meningioma tissues representing all WHO grades. Conclusion 800CW-TATE demonstrated sufficient binding affinity, specific SSTR2-mediated tumor uptake, a favorable biodistribution, and high TBR. These features make this tracer very promising for use in MFGS and could potentially aid in safer and a more complete meningioma resection, especially in high-grade meningiomas or those at complex anatomical localizations.

scholarly journals Molecular fluorescence-guided surgery of peritoneal carcinomatosis of colorectal origin: A narrative review

2018 ◽  
Vol 118 (2) ◽  
pp. 332-343 ◽  
Author(s):  
Judith E.K.R. Hentzen ◽  
Steven J. de Jongh ◽  
Patrick H.J. Hemmer ◽  
Willemijn Y. van der Plas ◽  
Gooitzen M. van Dam ◽  
...  
Keyword(s):  
Peritoneal Carcinomatosis ◽  
Narrative Review ◽  
Guided Surgery ◽  
Fluorescence Guided Surgery ◽  
Molecular Fluorescence ◽  
Colorectal Origin

scholarly journals Molecular fluorescence-guided surgery of peritoneal carcinomatosis of colorectal origin: a single-centre feasibility study

2016 ◽  
Vol 1 (4) ◽  
pp. 283-290 ◽  
Author(s):  
Niels J Harlaar ◽  
Marjory Koller ◽  
Steven J de Jongh ◽  
Barbara L van Leeuwen ◽  
Patrick H Hemmer ◽  
...  
Keyword(s):  
Peritoneal Carcinomatosis ◽  
Feasibility Study ◽  
Single Centre ◽  
Guided Surgery ◽  
Fluorescence Guided Surgery ◽  
Molecular Fluorescence ◽  
Colorectal Origin

Optical imaging probes and their potential contribution to radiotracer development

2016 ◽  
Vol 55 (02) ◽  
pp. 51-62 ◽  
Author(s):  
S. Hermann ◽  
M. Schäfers ◽  
C. Höltke ◽  
A. Faust
Keyword(s):  
Optical Imaging ◽  
Fluorescent Dyes ◽  
Great Influence ◽  
Potential Contribution ◽  
Preclinical Evaluation ◽  
Guided Surgery ◽  
Fluorescence Guided Surgery ◽  
Imaging Probes ◽  
Unspecific Binding

SummaryOptical imaging has long been considered a method for histological or microscopic investigations. Over the last 15 years, however, this method was applied for preclinical molecular imaging and, just recently, was also able to show its principal potential for clinical applications (e.g. fluorescence-guided surgery). Reviewing the development and preclinical evaluation of new fluorescent dyes and target-specific dye conjugates, these often show characteristic patterns of their routes of excretion and biodistribution, which could also be interesting for the development and optimization of radiopharmaceuticals. Especially ionic charges show a great influence on biodistribution and netcharge and charge-distribution on a conjugate often determines unspecific binding or background signals in liver, kidney or intestine, and other organs.Learning from fluorescent probe behaviour in vivo and translating this knowledge to radio-pharmaceuticals might be useful to further optimize emerging and existing radiopharmaceuticals with respect to their biodistribution and thereby availability for binding to their targets.

Unexpected Binding of Tozuleristide “Tumor Paint” to Cerebral Vascular Malformations: A Potentially Novel Application of Fluorescence-Guided Surgery

Neurosurgery ◽  
2021 ◽  
Author(s):  
Andrew J Kobets ◽  
David Nauen ◽  
Amy Lee ◽  
Alan R Cohen
Keyword(s):  
Near Infrared ◽  
Vascular Malformations ◽  
Cns Tumors ◽  
Matrix Metalloproteinase 2 ◽  
Cerebrovascular Lesions ◽  
Cavernous Malformations ◽  
Guided Surgery ◽  
Fluorescence Guided Surgery ◽  
Cerebral Vascular Malformations ◽  
Cerebral Vascular

Abstract BACKGROUND Fluorescence-guided surgery (FGS) is under investigation as a means to improve the extent of resection for primary central nervous system (CNS) tumors. Tozuleristide, known also as “Tumor Paint,” is an investigational tumor-targeting agent covalently conjugated to a derivative of the fluorescent dye indocyanine green. OBJECTIVE To report the finding of avid intraoperative fluorescence of tozuleristide on cerebral vascular malformations. METHODS Our institution is participating in a phase 2/3 study of intraoperative near-infrared fluorescence detection of pediatric primary CNS tumors in patients receiving intravenous tozuleristide and imaged with the Canvas system. Our site enrolled 2 patients with intracranial lesions, suspected preoperatively of possibly being gliomas that proved to be cavernous vascular malformations after resection. RESULTS Each lesion had a dark blue mulberry appearance and each fluoresced avidly with tozuleristide. Each was completely resected, and the patients recovered without deficit. Pathological assessment showed cavernous angioma for both cases. Tozuleristide fluorescence is postulated to result from binding to matrix metalloproteinase-2 and annexin A2, and literature review demonstrates expression of both these ligands on multiple cerebrovascular lesions, including cavernous malformations. CONCLUSION This finding deserves further investigation to determine if tozuleristide “Tumor Paint” may have a wider role in the identification of non-neoplastic intracranial pathologies.

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