scholarly journals A novel glioblastoma invasion model using human brain slice cultures

2021 ◽  
Vol 1 ◽  
pp. 100823
Author(s):  
V.M. Ravi ◽  
K. Joseph ◽  
J. Beck ◽  
U.G. Hofmann ◽  
O. Schnell ◽  
...  
Keyword(s):  
Human Brain ◽  
Brain Slice ◽  
Invasion Model ◽  
Brain Slice Cultures

scholarly journals Author response: Long-term adult human brain slice cultures as a model system to study human CNS circuitry and disease

2019 ◽  
Author(s):  
Niklas Schwarz ◽  
Betül Uysal ◽  
Marc Welzer ◽  
Jacqueline C Bahr ◽  
Nikolas Layer ◽  
...  
Keyword(s):  
Human Brain ◽  
Brain Slice ◽  
Model System ◽  
Author Response ◽  
Adult Human Brain ◽  
Adult Human ◽  
Brain Slice Cultures

scholarly journals Long-term adult human brain slice cultures as a model system to study human CNS circuitry and disease

eLife ◽  
2019 ◽  
Vol 8 ◽  
Author(s):  
Niklas Schwarz ◽  
Betül Uysal ◽  
Marc Welzer ◽  
Jacqueline C Bahr ◽  
Nikolas Layer ◽  
...  
Keyword(s):  
Human Brain ◽  
Brain Slice ◽  
Pyramidal Neurons ◽  
Model System ◽  
Model Systems ◽  
Human Model ◽  
Model Organisms ◽  
Brain Slice Cultures

Most of our knowledge on human CNS circuitry and related disorders originates from model organisms. How well such data translate to the human CNS remains largely to be determined. Human brain slice cultures derived from neurosurgical resections may offer novel avenues to approach this translational gap. We now demonstrate robust preservation of the complex neuronal cytoarchitecture and electrophysiological properties of human pyramidal neurons in long-term brain slice cultures. Further experiments delineate the optimal conditions for efficient viral transduction of cultures, enabling ‘high throughput’ fluorescence-mediated 3D reconstruction of genetically targeted neurons at comparable quality to state-of-the-art biocytin fillings, and demonstrate feasibility of long term live cell imaging of human cells in vitro. This model system has implications toward a broad spectrum of translational studies, regarding the validation of data obtained in non-human model systems, for therapeutic screening and genetic dissection of human CNS circuitry.

scholarly journals Organotypic brain slice cultures as a model to study angiogenesis of brain vessels

2015 ◽  
Vol 3 ◽  
Author(s):  
Bianca Hutter-Schmid ◽  
Kathrin M. Kniewallner ◽  
Christian Humpel
Keyword(s):  
Brain Slice ◽  
Brain Vessels ◽  
Organotypic Brain Slice ◽  
Brain Slice Cultures

scholarly journals Preparation of organotypic brain slice cultures for the study of Alzheimer’s disease

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 592
Author(s):  
Cara L. Croft ◽  
Wendy Noble
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Brain Slice ◽  
Synaptic Dysfunction ◽  
Slice Culture ◽  
Therapeutic Effects ◽  
Culture Model ◽  
Organotypic Brain Slice ◽  
Brain Slice Cultures

Alzheimer's disease, the most common cause of dementia, is a progressive neurodegenerative disorder characterised by amyloid-beta deposits in extracellular plaques, intracellular neurofibrillary tangles of aggregated tau, synaptic dysfunction and neuronal death. There are no cures for AD and current medications only alleviate some disease symptoms. Transgenic rodent models to study Alzheimer’s mimic features of human disease such as age-dependent accumulation of abnormal beta-amyloid and tau, synaptic dysfunction, cognitive deficits and neurodegeneration. These models have proven vital for improving our understanding of the molecular mechanisms underlying AD and for identifying promising therapeutic approaches. However, modelling neurodegenerative disease in animals commonly involves aging animals until they develop harmful phenotypes, often coupled with invasive procedures. In vivo studies are also resource, labour, time and cost intensive. We have developed a novel organotypic brain slice culture model to study Alzheimer’ disease which brings the potential of substantially reducing the number of rodents used in dementia research from an estimated 20,000 per year. We obtain 36 brain slices from each mouse pup, considerably reducing the numbers of animals required to investigate multiple stages of disease. This tractable model also allows the opportunity to modulate multiple pathways in tissues from a single animal. We believe that this model will most benefit dementia researchers in the academic and drug discovery sectors. We validated the slice culture model against aged mice, showing that the molecular phenotype closely mimics that displayed in vivo, albeit in an accelerated timescale. We showed beneficial outcomes following treatment of slices with agents previously shown to have therapeutic effects in vivo, and we also identified new mechanisms of action of other compounds. Thus, organotypic brain slice cultures from transgenic mouse models expressing Alzheimer’s disease-related genes may provide a valid and sensitive replacement for in vivo studies that do not involve behavioural analysis.

The Use of Brain Slice Cultures for the Study of Epilepsy

2006 ◽  
pp. 45-58
Author(s):  
SCOTT M. THOMPSON ◽  
XIANG CAI ◽  
CÉLINE DINOCOURT ◽  
MICHAEL W. NESTOR
Keyword(s):  
Brain Slice ◽  
Brain Slice Cultures

Three dimensional MEMS microfluidic perfusion system for thick brain slice cultures

2006 ◽  
Vol 9 (1) ◽  
pp. 7-13 ◽  
Author(s):  
Yoonsu Choi ◽  
Maxine A. McClain ◽  
Michelle C. LaPlaca ◽  
A. Bruno Frazier ◽  
Mark G. Allen
Keyword(s):  
Brain Slice ◽  
Three Dimensional ◽  
Perfusion System ◽  
Microfluidic Perfusion ◽  
Brain Slice Cultures ◽  
Microfluidic Perfusion System

Morphological effects of isoflavones (daidzein and genistein) on hypothalamic oxytocin neurons in the neonatal mouse brain slice cultures

2011 ◽  
Vol 505 (2) ◽  
pp. 87-92 ◽  
Author(s):  
Ryoichi Yoshimura ◽  
Erika Yamamoto ◽  
Yasuhisa Endo
Keyword(s):  
Mouse Brain ◽  
Brain Slice ◽  
Neonatal Mouse ◽  
Brain Slice Cultures

scholarly journals An in vitro blood-brain barrier model: Cocultures between endothelial cells and organotypic brain slice cultures

1998 ◽  
Vol 95 (4) ◽  
pp. 1840-1845 ◽  
Author(s):  
S. Duport ◽  
F. Robert ◽  
D. Muller ◽  
G. Grau ◽  
L. Parisi ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Blood Brain Barrier ◽  
Brain Slice ◽  
Brain Barrier ◽  
Blood Brain ◽  
Blood Brain Barrier Model ◽  
Organotypic Brain Slice ◽  
Brain Slice Cultures ◽  
Barrier Model
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