Proteomic analysis of endogenous conjugated linoleic acid biosynthesis in lactating rats and mouse mammary gland epithelia cells (HC11)

2010 ◽  
Vol 1804 (4) ◽  
pp. 745-751 ◽  
Author(s):  
Yong-Cheng Jin ◽  
Hong-Gu Lee ◽  
Cheng-Xiong Xu ◽  
Jeng-A Han ◽  
Seong-Ho Choi ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Proteomic Analysis ◽  
Mouse Mammary Gland ◽  
Acid Biosynthesis ◽  
Linoleic Acid Biosynthesis

scholarly journals Conjugated Linoleic Acid Induces Mast Cell Recruitment during Mouse Mammary Gland Stromal Remodeling

2007 ◽  
Vol 137 (5) ◽  
pp. 1200-1207 ◽  
Author(s):  
Joshua S. Russell ◽  
Sibel Oflazoglu McGee ◽  
Margot M. Ip ◽  
Dietrich Kuhlmann ◽  
Patricia A. Masso-Welch
Keyword(s):  
Mast Cell ◽  
Mammary Gland ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Mouse Mammary Gland ◽  
Cell Recruitment

scholarly journals Conjugated linoleic acid biosynthesis by human-derived Bifidobacterium species

2003 ◽  
Vol 94 (1) ◽  
pp. 138-145 ◽  
Author(s):  
M. Coakley ◽  
R.P. Ross ◽  
M. Nordgren ◽  
G. Fitzgerald ◽  
R. Devery ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Acid Biosynthesis ◽  
Bifidobacterium Species ◽  
Linoleic Acid Biosynthesis

Proteomic Analysis Reveals PGAM1 Altering cis-9, trans-11 Conjugated Linoleic Acid Synthesis in Bovine Mammary Gland

Lipids ◽  
2015 ◽  
Vol 50 (5) ◽  
pp. 469-481 ◽  
Author(s):  
T. Wang ◽  
S. B. Lee ◽  
J. H. Hwang ◽  
J. N. Lim ◽  
U. S. Jung ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Proteomic Analysis ◽  
Acid Synthesis ◽  
Bovine Mammary Gland

Conjugated Linoleic Acid: Biosynthesis and Nutritional Significance

2020 ◽  
pp. 67-106
Author(s):  
D. E. Bauman ◽  
A. L. Lock ◽  
R. Conboy Stephenson ◽  
K. Linehan ◽  
R. P. Ross ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Acid Biosynthesis ◽  
Linoleic Acid Biosynthesis

Independent effects of dietary linoleic and linolenic fatty acids on the conjugated linoleic acid content of cows’ milk

2002 ◽  
Vol 74 (1) ◽  
pp. 163-176 ◽  
Author(s):  
A.L. Lock ◽  
P.C. Garnsworthy
Keyword(s):  
Fatty Acids ◽  
Mammary Gland ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Linolenic Acid ◽  
Milk Fat ◽  
Latin Square ◽  
Health Benefit ◽  
Low Linolenic Acid ◽  
Low Linolenic

AbstractIt may be desirable to increase the level of conjugated linoleic acid (CLA) in milk as a health benefit in human nutrition. The purpose of this work was to separate the effects of linoleic and linolenic acids on CLA production in dairy cows and to determine to what extent endogenous synthesis contributes to cis-9, trans-11 CLA concentration in milk fat. Eight lactating cows and four non-lactating duodenal fistulated cows were used in a 4 ✕ 4 Latin-square design. All cows received a basal diet of grass silage that was supplemented with one of four concentrates, which were designed to differ in their linoleic and linolenic acid contents. The oil components of the concentrates were produced from mixtures of olive, linseed, rape, soya and sunflower oils to produce the four treatments: low linoleic/ low linolenic acid (LL), low linoleic/high linolenic acid (LH), high linoleic/low linolenic acid (HL) and high linoleic/ high linolenic acid (HH). Milk cis-9, trans-11 CLA contents were 0·8, 0·9, 0·9 and 1·1 g/100 g fatty acid methyl esters (P < 0·05) and yields were 5, 7, 7 and 8 g/day (P < 0·05) for the LL, LH, HL and HH treatments, respectively. The yields of trans-C18:1 fatty acids in milk were 19, 22, 21 and 23 g/day (P < 0·05), respectively. Taking the data for the cis-9, trans-11 CLA content and flow of duodenal fluid from the fistulated cows and representing this in terms of dietary intake by the lactating animals, the amounts of cis-9, trans-11 CLA produced in the rumen were calculated to be 0·8, 0·9, 1·2 and 1·1 g/day (P < 0·05) and for trans-C18:1 fatty acids 58, 58, 66 and 69 g/day (P < 0·05). Increasing linoleic and/or linolenic acids in the diet can increase the cis-9, trans-11 CLA content of cows’ milk. Only diets high in linoleic acid increased cis-9, trans-11 CLA production in the rumen. On all four diets, more than 80% of cis-9, trans-11 CLA in milk was produced endogenously by Δ9-desaturase from trans-11 C18:1 in the mammary gland. Cows on the same diet have different milk fat cis-9, trans-11 CLA concentrations that may be partially explained by differences in Δ9-desaturase activity between cows. Increasing the activity of Δ9-desaturase in the mammary gland may offer greater potential for enhancing the cis-9, trans-11 CLA content of milk fat than increasing cis-9, trans-11 CLA production in the rumen.

scholarly journals Conjugated Linoleic Acid–Enriched Butter Fat Alters Mammary Gland Morphogenesis and Reduces Cancer Risk in Rats

1999 ◽  
Vol 129 (12) ◽  
pp. 2135-2142 ◽  
Author(s):  
Clement Ip ◽  
Sebastiano Banni ◽  
Elisabetta Angioni ◽  
Gianfranca Carta ◽  
John McGinley ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Linoleic Acid ◽  
Cancer Risk ◽  
Conjugated Linoleic Acid ◽  
Butter Fat ◽  
Mammary Gland Morphogenesis ◽  
Gland Morphogenesis

scholarly journals Kinetics of trans-10, cis-12-conjugated linoleic acid transfer to plasma and milk following an abomasal bolus in lactating dairy cows

2018 ◽  
Vol 120 (3) ◽  
pp. 259-268 ◽  
Author(s):  
Natalie L. Urrutia ◽  
Monica Toledo ◽  
Michel Baldin ◽  
Jennifer L. Ford ◽  
Michael H. Green ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Milk Fat ◽  
Milk Fat Depression ◽  
Bolus Infusion ◽  
Bioactive Fatty Acids ◽  
Post Infusion ◽  
Kinetics Of ◽  
Transfer To Milk

AbstractTrans-10, cis-12-conjugated linoleic acid (CLA) is a potent bioactive fatty acids (FA) that causes milk fat depression in lactating animals. FA are transferred to milk directly through chylomicrons and indirectly by recycling through other tissues. The objective of this study was to characterise the kinetics of trans-10, cis-12 CLA transfer to plasma and milk after a single bolus infusion. Five multiparous mid-lactation cows received a single abomasal bolus infusion of an enriched CLA mixture providing 15 g of trans-10, cis-12 CLA and 15 g of cis-9, trans-11 CLA over a 30-min period. Plasma concentration of trans-10, cis-12 and cis-9, trans-11 CLA peaked 2 h post-bolus, reaching 0·29 and 0·38 % of total plasma FA, respectively, and returned to pre-bolus values at 72 h post-infusion. Milk trans-10, cis-12 CLA yield and concentration peaked 14 h post-bolus (0·25 g/h) and was not detectable in milk after 86 h. Total apparent transfer of trans-10, cis-12 CLA to milk was 41 %, with 73 % transferred to milk through the direct pool (chylomicrons) and the remaining 27 % transferred through the indirect pool (tissue recycling). Compartmental modelling revealed the existence of a transient unavailable pool of trans-10, cis-12 CLA in extravascular tissues represented primarily by the mammary gland, which slowly exchanges with an available pool for secretion in milk fat and transfer to milk. In conclusion, trans-10, cis-12 CLA is predominantly transferred to milk through the direct pathway; however, how this CLA isomer is processed within the mammary gland requires further investigation.

scholarly journals Trans-10, cis-12 conjugated linoleic acid reduces milk fat content and lipogenic gene expression in the mammary gland of sows without altering litter performance

2019 ◽  
Vol 123 (6) ◽  
pp. 610-618 ◽  
Author(s):  
E. C. Sandri ◽  
K. J. Harvatine ◽  
D. E. Oliveira
Keyword(s):  
Gene Expression ◽  
Mammary Gland ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Milk Fat ◽  
Fat Content ◽  
Lipogenic Gene ◽  
Lipogenic Genes ◽  
Lipogenic Gene Expression

AbstractTrans-10, cis-12 conjugated linoleic acid (CLA) decreases milk fat synthesis in lactating sows and involves, at least in part, the down-regulation of lipogenic genes. The objective was to evaluate the effect of CLA on milk composition and lipogenic gene expression. Twenty multiparous sows were randomly assigned to one of the two treatments for 18 d (from day 7 to day 25 of lactation): (1) control (no CLA added) and (2) 1 % of CLA mixed into the ration. CLA treatment decreased milk fat and protein content by 20 % (P = 0·004) and 11 % (P = 0·0001), respectively. However, piglet weight did not differ between treatments (P = 0·60). Dietary CLA increased the concentration of SFA in milk fat by 16 % (P < 0·0001) and decreased MUFA by 17·6 % (P < 0·0001). In the mammary gland, CLA reduced gene expression of acetyl-CoA carboxylase-α by 37 % (P = 0·003), fatty acid synthase by 64 % (P = 0·002), stearoyl-CoA desaturase 1 by 52 % (P = 0·003), lipoprotein lipase by 26 % (P = 0·03), acyl glycerol phosphate acyltransferase 6 by 15 % (P = 0·02) and diacylglycerol acyltransferase 1 by 27 % (P = 0·02), whereas the expression of fatty acid binding protein 3 was not altered by CLA treatment (P = 0·09). Mammary expression of casein-β and α-lactalbumin was reduced by CLA by 68 % (P = 0·0004) and 62 % (P = 0·005), respectively. Additionally, CLA had no effect on the expression of lipogenic genes evaluated in adipose tissue. In summary, CLA reduced milk fat content without negatively affecting litter performance and it affected mammary expression of genes involved in all lipogenic pathways studied.

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