scholarly journals Characterization of gene–environment interactions by behavioral profiling of selectively bred rats: The effect of NMDA receptor inhibition and social isolation

2013 ◽  
Vol 240 ◽  
pp. 134-145 ◽  
Author(s):  
Zita Petrovszki ◽  
Gabor Adam ◽  
Gabor Tuboly ◽  
Gabriella Kekesi ◽  
Gyorgy Benedek ◽  
...  
Keyword(s):  
Nmda Receptor ◽  
Social Isolation ◽  
Gene Environment ◽  
Receptor Inhibition ◽  
Behavioral Profiling

Mechanisms of NMDA receptor inhibition by diarylamidine compounds

2019 ◽  
Vol 51 (7) ◽  
pp. 1573-1582 ◽  
Author(s):  
Mikhail Y. Dron ◽  
Arseniy S. Zhigulin ◽  
Oleg I. Barygin
Keyword(s):  
Nmda Receptor ◽  
Receptor Inhibition

scholarly journals Biochemical and electrophysiological characterization of N- glycans on NMDA receptor subunits

2016 ◽  
Vol 138 (4) ◽  
pp. 546-556 ◽  
Author(s):  
Martina Kaniakova ◽  
Katarina Lichnerova ◽  
Kristyna Skrenkova ◽  
Ladislav Vyklicky ◽  
Martin Horak
Keyword(s):  
Nmda Receptor ◽  
Nmda Receptor Subunits ◽  
Electrophysiological Characterization

scholarly journals Characterization of the 6'-N-aminoglycoside acetyltransferase gene aac(6')-Im [corrected] associated with a sulI-type integron.

1997 ◽  
Vol 41 (2) ◽  
pp. 314-318 ◽  
Author(s):  
E Hannecart-Pokorni ◽  
F Depuydt ◽  
L de wit ◽  
E van Bossuyt ◽  
J Content ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Amino Acids ◽  
Amino Acid ◽  
Resistance Gene ◽  
Citrobacter Freundii ◽  
Gram Negative ◽  
Gene Environment ◽  
Insert Region ◽  
Klebsiella Spp

The amikacin resistance gene aac(6')-Im [corrected] from Citrobacter freundii Cf155 encoding an aminoglycoside 6'-N-acetyltransferase was characterized. The gene was identified as a coding sequence of 521 bp located down-stream from the 5' conserved segment of an integron. The sequence of this aac(6')-Im [corrected] gene corresponded to a protein of 173 amino acids which possessed 64.2% identity in a 165-amino-acid overlap with the aac(6')-Ia gene product (F.C. Tenover, D. Filpula, K.L. Phillips, and J. J. Plorde, J. Bacteriol. 170:471-473, 1988). By using PCR, the aac(6')-Im [corrected] gene could be detected in 8 of 86 gram-negative clinical isolates from two Belgian hospitals, including isolates of Citrobacter, Klebsiella spp., and Escherichia coli. PCR mapping of the aac(6')-Im [corrected] gene environment in these isolates indicated that the gene was located within a sulI-type integron; the insert region is 1,700 bases long and includes two genes cassettes, the second being ant (3")-Ib.

scholarly journals Classifying neuronal subclasses of the cerebellum through constellation pharmacology

2016 ◽  
Vol 115 (2) ◽  
pp. 1031-1042 ◽  
Author(s):  
Kigen J. Curtice ◽  
Lee S. Leavitt ◽  
Kevin Chase ◽  
Shrinivasan Raghuraman ◽  
Martin P. Horvath ◽  
...  
Keyword(s):  
Nmda Receptor ◽  
Gaba Receptors ◽  
Acetylcholine Receptors ◽  
Granule Cells ◽  
Functional Expression ◽  
Muscarinic Acetylcholine Receptors ◽  
Receptor Subtypes ◽  
Mouse Cerebellum ◽  
The Brain

A pressing need in neurobiology is the comprehensive identification and characterization of neuronal subclasses within the mammalian nervous system. To this end, we used constellation pharmacology as a method to interrogate the neuronal and glial subclasses of the mouse cerebellum individually and simultaneously. We then evaluated the data obtained from constellation-pharmacology experiments by cluster analysis to classify cells into neuronal and glial subclasses, based on their functional expression of glutamate, acetylcholine, and GABA receptors, among other ion channels. Conantokin peptides were used to identify N-methyl-d-aspartate (NMDA) receptor subtypes, which revealed that neurons of the young mouse cerebellum expressed NR2A and NR2B NMDA receptor subunits. Additional pharmacological tools disclosed differential expression of α-amino-3-hydroxy-5-methyl-4-isoxazloepropionic, nicotinic acetylcholine, and muscarinic acetylcholine receptors in different neuronal and glial subclasses. Certain cell subclasses correlated with known attributes of granule cells, and we combined constellation pharmacology with genetically labeled neurons to identify and characterize Purkinje cells. This study illustrates the utility of applying constellation pharmacology to classify neuronal and glial subclasses in specific anatomical regions of the brain.

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