Characterization of green mutants in Fremyella diplosiphon provides insight into the impact of phycoerythrin deficiency and linker function on complementary chromatic adaptation

Thermo-setting polymers are widely used as underfill materials to improve the reliability of electronic packages. In the design phase, the influence of underfill applications on reliability is often judged through thermal and mechanical simulations, under assumed operating conditions. Because of lacking insight into the mechanical processes due to polymer curing, the impact of processing induced residual stress fields is often neglected. To investigate the evolution of stress and strain fields during the curing process it is important to assume a more appropriate starting point for subsequent process modeling. Furthermore, study of possible damage originating from the fabrication process then comes within reach. To facilitate future analysis of stress and strain fields during the curing process a cure dependent constitutive relation is assumed. An approximate investigation method for the process-dependent mechanical properties, based on Dynamic Mechanic Analysis (DMA), is developed. As an illustration the parameter identification is performed for a selected epoxy resin.

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COMPLEMENTARY CHROMATIC ADAPTATION IN A FILAMENTOUS BLUE-GREEN ALGA

The Journal of Cell Biology ◽

10.1083/jcb.58.2.419 ◽

1973 ◽

Vol 58 (2) ◽

pp. 419-435 ◽

Cited By ~ 767

Author(s):

Allen Bennett ◽

Lawrence Bogorad

Keyword(s):

De Novo ◽

Red Light ◽

Experimental System ◽

Chromatic Adaptation ◽

Fluorescent Light ◽

Filament Length ◽

Fremyella Diplosiphon ◽

Complementary Chromatic Adaptation ◽

Metabolic Degradation ◽

Light Transfer

Fluorescent and red light environments generate greatly different patterns of pigmentation and morphology in Fremyella diplosiphon. Most strikingly, red-illuminated cultures contain no measurable C-phycoerythrin and have a mean filament length about 10 times shorter than fluorescent-illuminated cultures. C-phycoerythrin behaves as a photoinducible constituent of this alga. Spectrophotometric and immunochemical procedures were devised so that C-phycoerythrin metabolism could be studied quantitatively with [14C]-phenylalanine pulse-chased cultures. Transfer of red-illuminated cultures to fluorescent light initiates C-phycoerythrin production by essentially de novo synthesis. C-phycoerythrin is not degraded to any significant extent in cultures continuously illuminated with fluorescent light. Transfer of fluorescent-illuminated cultures to red light causes an abrupt cessation of C-phycoerythrin synthesis. The C-phycoerythrin content of cultures adapting to red light decreases and subsequently becomes constant. Loss of C-phycoerythrin is not brought about by metabolic degradation, but rather by a decrease in mean filament length which is effected by transcellular breakage. In this experimental system, light influences intracellular C-phycoerythrin levels by regulating the rate of synthesis of the chromoprotein.

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Characterization of nuclear mitochondrial insertions in the whole genomes of primates

NAR Genomics and Bioinformatics ◽

10.1093/nargab/lqaa089 ◽

2020 ◽

Vol 2 (4) ◽

Author(s):

Gargi Dayama ◽

Weichen Zhou ◽

Javier Prado-Martinez ◽

Tomas Marques-Bonet ◽

Ryan E Mills

Keyword(s):

Sequence Data ◽

World Monkey ◽

Great Apes ◽

Human Genomes ◽

Whole Genomes ◽

Fixed Reference ◽

Cellular Pathways ◽

The Impact ◽

Insight Into

Abstract The transfer and integration of whole and partial mitochondrial genomes into the nuclear genomes of eukaryotes is an ongoing process that has facilitated the transfer of genes and contributed to the evolution of various cellular pathways. Many previous studies have explored the impact of these insertions, referred to as NumtS, but have focused primarily on older events that have become fixed and are therefore present in all individual genomes for a given species. We previously developed an approach to identify novel Numt polymorphisms from next-generation sequence data and applied it to thousands of human genomes. Here, we extend this analysis to 79 individuals of other great ape species including chimpanzee, bonobo, gorilla, orang-utan and also an old world monkey, macaque. We show that recent Numt insertions are prevalent in each species though at different apparent rates, with chimpanzees exhibiting a significant increase in both polymorphic and fixed Numt sequences as compared to other great apes. We further assessed positional effects in each species in terms of evolutionary time and rate of insertion and identified putative hotspots on chromosome 5 for Numt integration, providing insight into both recent polymorphic and older fixed reference NumtS in great apes in comparison to human events.

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Characterization of nuclear mitochondrial insertions in the whole genomes of primates

10.1101/2020.02.24.963504 ◽

2020 ◽

Author(s):

Gargi Dayama ◽

Weichen Zhou ◽

Javier Prado-Martinez ◽

Tomas Marques-Bonet ◽

Ryan E. Mills

Keyword(s):

Sequence Data ◽

World Monkey ◽

Great Apes ◽

Human Genomes ◽

Whole Genomes ◽

Fixed Reference ◽

Cellular Pathways ◽

The Impact ◽

Insight Into

ABSTRACTThe transfer and integration of whole and partial mitochondrial genomes into the nuclear genomes of eukaryotes is an ongoing process that has facilitated the transfer of genes and contributed to the evolution of various cellular pathways. Many previous studies have explored the impact of these insertions, referred to as NumtS, but have focused primarily on older events that have become fixed and are therefore present in all individual genomes for a given species. We previously developed an approach to identify novel Numt polymorphisms from next generation sequence data and applied it to thousands of human genomes. Here, we extend this analysis to 79 individuals of other great ape species including chimpanzee, bonobo, gorilla, orang-utan and also an old world monkey, macaque. We show that recent Numt insertions are prevalent in each species though at different apparent rates, with chimpanzees exhibiting a significant increase in both polymorphic and fixed Numt sequences as compared to other great apes. We further assessed positional effects in each species in terms of evolutionary time and rate of insertion and identified putative hotspots on chromosome 5 for Numt integration, providing insight into both recent polymorphic and older fixed reference NumtS in great apes in comparison to human events.

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Photoregulation of Cellular Morphology during Complementary Chromatic Adaptation Requires Sensor-Kinase-Class Protein RcaE in Fremyella diplosiphon

Journal of Bacteriology ◽

10.1128/jb.00018-08 ◽

2008 ◽

Vol 190 (11) ◽

pp. 4069-4074 ◽

Cited By ~ 54

Author(s):

Juliana R. Bordowitz ◽

Beronda L. Montgomery

Keyword(s):

Mutant Strain ◽

Molecular Basis ◽

Green Light ◽

Cellular Morphology ◽

Chromatic Adaptation ◽

Sensor Kinase ◽

Wild Type ◽

Fremyella Diplosiphon ◽

Complementary Chromatic Adaptation ◽

Biochemical Analyses

ABSTRACT We used wild-type UTEX481; SF33, a shortened-filament mutant strain that shows normal complementary chromatic adaptation pigmentation responses; and FdBk14, an RcaE-deficient strain that lacks light-dependent pigmentation responses, to investigate the molecular basis of the photoregulation of cellular morphology in the cyanobacterium Fremyella diplosiphon. Detailed microscopic and biochemical analyses indicate that RcaE is required for the photoregulation of cell and filament morphologies of F. diplosiphon in response to red and green light.

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