scholarly journals CotB is essential for complete activation of green light‐induced genes during complementary chromatic adaptation in Fremyella diplosiphon

2003 ◽  
Vol 50 (3) ◽  
pp. 781-793 ◽  
Author(s):  
Barbara E. Balabas ◽  
Beronda L. Montgomery ◽  
Laura E. Ong ◽  
David M. Kehoe
Keyword(s):  
Green Light ◽  
Chromatic Adaptation ◽  
Fremyella Diplosiphon ◽  
Complementary Chromatic Adaptation ◽  
Induced Genes

scholarly journals Photoregulation of Cellular Morphology during Complementary Chromatic Adaptation Requires Sensor-Kinase-Class Protein RcaE in Fremyella diplosiphon

2008 ◽  
Vol 190 (11) ◽  
pp. 4069-4074 ◽  
Author(s):  
Juliana R. Bordowitz ◽  
Beronda L. Montgomery
Keyword(s):  
Mutant Strain ◽  
Molecular Basis ◽  
Green Light ◽  
Cellular Morphology ◽  
Chromatic Adaptation ◽  
Sensor Kinase ◽  
Wild Type ◽  
Fremyella Diplosiphon ◽  
Complementary Chromatic Adaptation ◽  
Biochemical Analyses

ABSTRACT We used wild-type UTEX481; SF33, a shortened-filament mutant strain that shows normal complementary chromatic adaptation pigmentation responses; and FdBk14, an RcaE-deficient strain that lacks light-dependent pigmentation responses, to investigate the molecular basis of the photoregulation of cellular morphology in the cyanobacterium Fremyella diplosiphon. Detailed microscopic and biochemical analyses indicate that RcaE is required for the photoregulation of cell and filament morphologies of F. diplosiphon in response to red and green light.

scholarly journals FdTonB is involved in the photoregulation of cellular morphology during complementary chromatic adaptation in Fremyella diplosiphon

Microbiology ◽  
2010 ◽  
Vol 156 (3) ◽  
pp. 731-741 ◽  
Author(s):  
Bagmi Pattanaik ◽  
Beronda L. Montgomery
Keyword(s):  
Transmembrane Domain ◽  
Sequence Similarity ◽  
Green Light ◽  
Cellular Morphology ◽  
Chromatic Adaptation ◽  
Cell Width ◽  
Fremyella Diplosiphon ◽  
Complementary Chromatic Adaptation ◽  
Rich Domain ◽  
Sequence Similarity Analysis

We have characterized a Fremyella diplosiphon TonB protein (FdTonB) and investigated its function during complementary chromatic adaptation. Sequence similarity analysis of FdTonB (571 aa) led to identification of several conserved domains characteristic of TonB proteins, including an N-terminal transmembrane domain, a central proline-rich spacer and a C-terminal TonB-related domain (TBRD). We identified a novel glycine-rich domain containing (Gly-X) n repeats. To assess FdTonB function, we constructed a ΔtonB mutant through homologous recombination based upon truncation of the central proline-rich spacer, glycine-rich domain and TBRD. Our ΔtonB mutant exhibited an aberrant cellular morphology under green light, with expanded cell width compared to the parental wild-type (WT) strain. The cellular morphology of the ΔtonB mutant recovered upon WT tonB expression. Interestingly, tonB expression was found to be independent of RcaE. As ΔtonB and WT strains respond in the same way when grown under iron-replete versus iron-limited conditions, our results suggest that FdTonB is not involved in the classic TonB function of mediating cellular adaptation to iron limitation, but exhibits a novel function related to the photoregulation of cellular morphology in F. diplosiphon.

A proteomic approach to the analysis of the components of the phycobilisomes from two cyanobacteria with complementary chromatic adaptation: Fremyella diplosiphon UTEX B590 and Tolypothrix PCC 7601

2012 ◽  
Vol 114 (1) ◽  
pp. 43-58 ◽  
Author(s):  
Bertha Pérez-Gómez ◽  
Guillermo Mendoza-Hernández ◽  
Tecilli Cabellos-Avelar ◽  
Lourdes Elizabeth Leyva-Castillo ◽  
Emma Berta Gutiérrez-Cirlos ◽  
...  
Keyword(s):  
Chromatic Adaptation ◽  
Fremyella Diplosiphon ◽  
Complementary Chromatic Adaptation ◽  
Proteomic Approach

scholarly journals COMPLEMENTARY CHROMATIC ADAPTATION IN A FILAMENTOUS BLUE-GREEN ALGA

1973 ◽  
Vol 58 (2) ◽  
pp. 419-435 ◽  
Author(s):  
Allen Bennett ◽  
Lawrence Bogorad
Keyword(s):  
De Novo ◽  
Red Light ◽  
Experimental System ◽  
Chromatic Adaptation ◽  
Fluorescent Light ◽  
Filament Length ◽  
Fremyella Diplosiphon ◽  
Complementary Chromatic Adaptation ◽  
Metabolic Degradation ◽  
Light Transfer

Fluorescent and red light environments generate greatly different patterns of pigmentation and morphology in Fremyella diplosiphon. Most strikingly, red-illuminated cultures contain no measurable C-phycoerythrin and have a mean filament length about 10 times shorter than fluorescent-illuminated cultures. C-phycoerythrin behaves as a photoinducible constituent of this alga. Spectrophotometric and immunochemical procedures were devised so that C-phycoerythrin metabolism could be studied quantitatively with [14C]-phenylalanine pulse-chased cultures. Transfer of red-illuminated cultures to fluorescent light initiates C-phycoerythrin production by essentially de novo synthesis. C-phycoerythrin is not degraded to any significant extent in cultures continuously illuminated with fluorescent light. Transfer of fluorescent-illuminated cultures to red light causes an abrupt cessation of C-phycoerythrin synthesis. The C-phycoerythrin content of cultures adapting to red light decreases and subsequently becomes constant. Loss of C-phycoerythrin is not brought about by metabolic degradation, but rather by a decrease in mean filament length which is effected by transcellular breakage. In this experimental system, light influences intracellular C-phycoerythrin levels by regulating the rate of synthesis of the chromoprotein.

scholarly journals A Turquoise Mutant Genetically Separates Expression of Genes Encoding Phycoerythrin and Its Associated Linker Peptides

2002 ◽  
Vol 184 (4) ◽  
pp. 962-970 ◽  
Author(s):  
Laura Ort Seib ◽  
David M. Kehoe
Keyword(s):  
Light Quality ◽  
Sequence Similarity ◽  
Red Light ◽  
Green Light ◽  
Transcript Abundance ◽  
Chromatic Adaptation ◽  
Partial Control ◽  
Complementary Chromatic Adaptation ◽  
Expression Of Genes ◽  
Genes Encoding

ABSTRACT During complementary chromatic adaptation (CCA), cyanobacterial light harvesting structures called phycobilisomes are restructured in response to ambient light quality shifts. Transcription of genes encoding components of the phycobilisome is differentially regulated during this process: red light activates cpcB2A2, whereas green light coordinately activates the cpeCDE and cpeBA operons. Three signal transduction components that regulate CCA have been isolated to date: a sensor-photoreceptor (RcaE) and two response regulators (RcaF and RcaC). Mutations in the genes encoding these components affect the accumulation of both cpcB2A2 and cpeBA gene products. We have isolated and characterized a new pigmentation mutant called Turquoise 1. We demonstrate that this mutant phenotype is due to a dramatic decrease in cpeBA transcript abundance and results from a lesion in the cpeR gene. However, in this mutant cpeCDE RNA levels remain near those found in wild-type cells. Our results show that the coordinate regulation of cpeBA and cpeCDE by green light can be uncoupled by the loss of CpeR, and we furnish the first genetic evidence that different regulatory mechanisms control these two operons. Sequence analysis of CpeR reveals that it shares limited sequence similarity to members of the PP2C class of protein serine/threonine phosphatases. We also demonstrate that cpeBA and cpeCDE retain light quality responsiveness in a mutant lacking the RcaE photoreceptor. This provides compelling evidence for the partial control of CCA through an as-yet-uncharacterized second light quality sensing system.

Sensing of Green Light in Complementary Chromatic Adaptation of the Cyanobacterium Calothrix sp.

1999 ◽  
pp. 187-194
Author(s):  
Hans C. P. Matthijs ◽  
Jeroen H. Geerdink ◽  
Hans Balke ◽  
Andrea Haker ◽  
Hendrik Schubert ◽  
...  
Keyword(s):  
Green Light ◽  
Chromatic Adaptation ◽  
Complementary Chromatic Adaptation
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