scholarly journals COMPLEMENTARY CHROMATIC ADAPTATION IN A FILAMENTOUS BLUE-GREEN ALGA

1973 ◽  
Vol 58 (2) ◽  
pp. 419-435 ◽  
Author(s):  
Allen Bennett ◽  
Lawrence Bogorad
Keyword(s):  
De Novo ◽  
Red Light ◽  
Experimental System ◽  
Chromatic Adaptation ◽  
Fluorescent Light ◽  
Filament Length ◽  
Fremyella Diplosiphon ◽  
Complementary Chromatic Adaptation ◽  
Metabolic Degradation ◽  
Light Transfer

Fluorescent and red light environments generate greatly different patterns of pigmentation and morphology in Fremyella diplosiphon. Most strikingly, red-illuminated cultures contain no measurable C-phycoerythrin and have a mean filament length about 10 times shorter than fluorescent-illuminated cultures. C-phycoerythrin behaves as a photoinducible constituent of this alga. Spectrophotometric and immunochemical procedures were devised so that C-phycoerythrin metabolism could be studied quantitatively with [14C]-phenylalanine pulse-chased cultures. Transfer of red-illuminated cultures to fluorescent light initiates C-phycoerythrin production by essentially de novo synthesis. C-phycoerythrin is not degraded to any significant extent in cultures continuously illuminated with fluorescent light. Transfer of fluorescent-illuminated cultures to red light causes an abrupt cessation of C-phycoerythrin synthesis. The C-phycoerythrin content of cultures adapting to red light decreases and subsequently becomes constant. Loss of C-phycoerythrin is not brought about by metabolic degradation, but rather by a decrease in mean filament length which is effected by transcellular breakage. In this experimental system, light influences intracellular C-phycoerythrin levels by regulating the rate of synthesis of the chromoprotein.

RcaE is a complementary chromatic adaptation photoreceptor required for green and red light responsiveness

2004 ◽  
Vol 51 (2) ◽  
pp. 567-577 ◽  
Author(s):  
Kazuki Terauchi ◽  
Beronda L. Montgomery ◽  
Arthur R. Grossman ◽  
J. Clark Lagarias ◽  
David M. Kehoe
Keyword(s):  
Red Light ◽  
Chromatic Adaptation ◽  
Complementary Chromatic Adaptation

A proteomic approach to the analysis of the components of the phycobilisomes from two cyanobacteria with complementary chromatic adaptation: Fremyella diplosiphon UTEX B590 and Tolypothrix PCC 7601

2012 ◽  
Vol 114 (1) ◽  
pp. 43-58 ◽  
Author(s):  
Bertha Pérez-Gómez ◽  
Guillermo Mendoza-Hernández ◽  
Tecilli Cabellos-Avelar ◽  
Lourdes Elizabeth Leyva-Castillo ◽  
Emma Berta Gutiérrez-Cirlos ◽  
...  
Keyword(s):  
Chromatic Adaptation ◽  
Fremyella Diplosiphon ◽  
Complementary Chromatic Adaptation ◽  
Proteomic Approach

scholarly journals Genomic DNA Microarray Analysis: Identification of New Genes Regulated by Light Color in the Cyanobacterium Fremyella diplosiphon

2004 ◽  
Vol 186 (13) ◽  
pp. 4338-4349 ◽  
Author(s):  
Emily L. Stowe-Evans ◽  
James Ford ◽  
David M. Kehoe
Keyword(s):  
Genomic Dna ◽  
Dna Microarrays ◽  
Chlorophyll Biosynthesis ◽  
Red Light ◽  
Chromatic Adaptation ◽  
Light Spectrum ◽  
Two Dimensional Gel Electrophoresis ◽  
Fremyella Diplosiphon ◽  
New Genes ◽  
Light Color

ABSTRACT Many cyanobacteria use complementary chromatic adaptation to efficiently utilize energy from both green and red regions of the light spectrum during photosynthesis. Although previous studies have shown that acclimation to changing light wavelengths involves many physiological responses, research to date has focused primarily on the expression and regulation of genes that encode proteins of the major photosynthetic light-harvesting antennae, the phycobilisomes. We have used two-dimensional gel electrophoresis and genomic DNA microarrays to expand our understanding of the physiology of acclimation to light color in the cyanobacterium Fremyella diplosiphon. We found that the levels of nearly 80 proteins are altered in cells growing in green versus red light and have cloned and positively identified 17 genes not previously known to be regulated by light color in any species. Among these are homologs of genes present in many bacteria that encode well-studied proteins lacking clearly defined functions, such as tspO, which encodes a tryptophan-rich sensory protein, and homologs of genes encoding proteins of clearly defined function in many species, such as nblA and chlL, encoding phycobilisome degradation and chlorophyll biosynthesis proteins, respectively. Our results suggest novel roles for several of these gene products and highly specialized, unique uses for others.

scholarly journals In the Limelight: Photoreceptors in Cyanobacteria

mBio ◽  
2016 ◽  
Vol 7 (3) ◽  
Author(s):  
Devaki Bhaya
Keyword(s):  
Color Change ◽  
Red Light ◽  
Signal Transduction Pathway ◽  
Fine Tuning ◽  
Chromatic Adaptation ◽  
Complex Signal ◽  
Quality Change ◽  
Biotechnological Potential ◽  
Complementary Chromatic Adaptation ◽  
Light Levels

ABSTRACT Certain cyanobacteria look green if grown in red light and vice versa. This dramatic color change, called complementary chromatic adaptation (CCA), is caused by alterations of the major colored light-harvesting proteins. A major controller of CCA is the cyanobacteriochrome (CBCR) RcaE, a red-green reversible photoreceptor that triggers a complex signal transduction pathway. Now, a new study demonstrates that CCA is also modulated by DpxA, a CBCR that senses yellow and teal (greenish blue) light. DpxA acts to expand the range of wavelengths that can impact CCA, by fine-tuning the process. This dual control of CCA might positively impact the fitness of cells growing in the shade of competing algae or in a water column where light levels and spectral quality change gradually with depth. This discovery adds to the growing number of light-responsive phenomena controlled by multiple CBCRs. Furthermore, the diverse CBCRs which are exclusively found in cyanobacteria have significant biotechnological potential.

scholarly journals A Turquoise Mutant Genetically Separates Expression of Genes Encoding Phycoerythrin and Its Associated Linker Peptides

2002 ◽  
Vol 184 (4) ◽  
pp. 962-970 ◽  
Author(s):  
Laura Ort Seib ◽  
David M. Kehoe
Keyword(s):  
Light Quality ◽  
Sequence Similarity ◽  
Red Light ◽  
Green Light ◽  
Transcript Abundance ◽  
Chromatic Adaptation ◽  
Partial Control ◽  
Complementary Chromatic Adaptation ◽  
Expression Of Genes ◽  
Genes Encoding

ABSTRACT During complementary chromatic adaptation (CCA), cyanobacterial light harvesting structures called phycobilisomes are restructured in response to ambient light quality shifts. Transcription of genes encoding components of the phycobilisome is differentially regulated during this process: red light activates cpcB2A2, whereas green light coordinately activates the cpeCDE and cpeBA operons. Three signal transduction components that regulate CCA have been isolated to date: a sensor-photoreceptor (RcaE) and two response regulators (RcaF and RcaC). Mutations in the genes encoding these components affect the accumulation of both cpcB2A2 and cpeBA gene products. We have isolated and characterized a new pigmentation mutant called Turquoise 1. We demonstrate that this mutant phenotype is due to a dramatic decrease in cpeBA transcript abundance and results from a lesion in the cpeR gene. However, in this mutant cpeCDE RNA levels remain near those found in wild-type cells. Our results show that the coordinate regulation of cpeBA and cpeCDE by green light can be uncoupled by the loss of CpeR, and we furnish the first genetic evidence that different regulatory mechanisms control these two operons. Sequence analysis of CpeR reveals that it shares limited sequence similarity to members of the PP2C class of protein serine/threonine phosphatases. We also demonstrate that cpeBA and cpeCDE retain light quality responsiveness in a mutant lacking the RcaE photoreceptor. This provides compelling evidence for the partial control of CCA through an as-yet-uncharacterized second light quality sensing system.

scholarly journals Photoregulation of Cellular Morphology during Complementary Chromatic Adaptation Requires Sensor-Kinase-Class Protein RcaE in Fremyella diplosiphon

2008 ◽  
Vol 190 (11) ◽  
pp. 4069-4074 ◽  
Author(s):  
Juliana R. Bordowitz ◽  
Beronda L. Montgomery
Keyword(s):  
Mutant Strain ◽  
Molecular Basis ◽  
Green Light ◽  
Cellular Morphology ◽  
Chromatic Adaptation ◽  
Sensor Kinase ◽  
Wild Type ◽  
Fremyella Diplosiphon ◽  
Complementary Chromatic Adaptation ◽  
Biochemical Analyses

ABSTRACT We used wild-type UTEX481; SF33, a shortened-filament mutant strain that shows normal complementary chromatic adaptation pigmentation responses; and FdBk14, an RcaE-deficient strain that lacks light-dependent pigmentation responses, to investigate the molecular basis of the photoregulation of cellular morphology in the cyanobacterium Fremyella diplosiphon. Detailed microscopic and biochemical analyses indicate that RcaE is required for the photoregulation of cell and filament morphologies of F. diplosiphon in response to red and green light.

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