V-ATPase-dependent luminal acidification is required for endocytic recycling of a yeast cell wall stress sensor, Wsc1p

2014 ◽  
Vol 443 (2) ◽  
pp. 549-555 ◽  
Author(s):  
Kazuma Ueno ◽  
Mayu Saito ◽  
Makiko Nagashima ◽  
Ai Kojima ◽  
Show Nishinoaki ◽  
...  
Keyword(s):  
Cell Wall ◽  
Yeast Cell ◽  
Wall Stress ◽  
Yeast Cell Wall ◽  
Endocytic Recycling ◽  
Stress Sensor ◽  
Cell Wall Stress

scholarly journals NPFXD-mediated Endocytosis Is Required for Polarity and Function of a Yeast Cell Wall Stress Sensor

2007 ◽  
Vol 18 (1) ◽  
pp. 57-65 ◽  
Author(s):  
Hai Lan Piao ◽  
Iara M.P. Machado ◽  
Gregory S. Payne
Keyword(s):  
Cell Wall ◽  
Yeast Cell ◽  
Wall Stress ◽  
Yeast Cell Wall ◽  
Stress Sensor ◽  
Cell Wall Stress ◽  
A Cell ◽  
Increased Sensitivity ◽  
Targeting Signal ◽  
And Function

The actin-associated protein Sla1p, through its SHD1 domain, acts as an adaptor for the NPFX(1,2)D endocytic targeting signal in yeast. Here we report that Wsc1p, a cell wall stress sensor, depends on this signal-adaptor pair for endocytosis. Mutation of NPFDD in Wsc1p or expression of Sla1p lacking SHD1 blocked Wsc1p internalization. By live cell imaging, endocytically defective Wsc1p was not concentrated at sites of endocytosis. Polarized distribution of Wsc1p to regions of cell growth was lost in the absence of endocytosis. Mutations in genes necessary for endosome to Golgi traffic caused redistribution of Wsc1p from the cell surface to internal compartments, indicative of recycling. Inhibition of Wsc1p endocytosis caused defects in polarized deposition of the cell wall and increased sensitivity to perturbation of cell wall synthesis. Our results reveal that the NPFX(1,2)D-Sla1p system is responsible for directing Wsc1p into an endocytosis and recycling pathway necessary to maintain yeast cell wall polarity. The dynamic localization of Wsc1p, a sensor of the extracellular wall in yeast, resembles polarized distribution of certain extracellular matrix-sensing integrins through endocytic recycling.

The putative stress sensor protein MtlA is required for conidia formation, cell wall stress tolerance, and cell wall integrity inAspergillus nidulans

2014 ◽  
Vol 78 (2) ◽  
pp. 326-335 ◽  
Author(s):  
Taiki Futagami ◽  
Kazufumi Seto ◽  
Yasuhiro Kajiwara ◽  
Hideharu Takashita ◽  
Toshiro Omori ◽  
...  
Keyword(s):  
Cell Wall ◽  
Stress Tolerance ◽  
Wall Stress ◽  
Cell Wall Integrity ◽  
Stress Sensor ◽  
Cell Wall Stress ◽  
Sensor Protein

Functional analysis of cell wall stress sensor proteins WscA and WscB from Aspergillus nidulans

2010 ◽  
Vol 150 ◽  
pp. 359-359
Author(s):  
Taiki Futagami ◽  
Yasuhiro Kajiwara ◽  
Hideharu Takashita ◽  
Toshiro Omori ◽  
Masatoshi Goto
Keyword(s):  
Functional Analysis ◽  
Cell Wall ◽  
Aspergillus Nidulans ◽  
Wall Stress ◽  
Stress Sensor ◽  
Cell Wall Stress ◽  
Sensor Proteins

In vitro Cell Wall Stress Assay for Fusarium oxysporum

BIO-PROTOCOL ◽  
2016 ◽  
Vol 6 (17) ◽  
Author(s):  
Elena Pérez-Nadales ◽  
Antonio Di Pietro
Keyword(s):  
Cell Wall ◽  
Fusarium Oxysporum ◽  
Wall Stress ◽  
Cell Wall Stress

scholarly journals Peptidogalactomannan from Histoplasma capsulatum yeast cell wall: role of the chemical structure in recognition and activation by peritoneal macrophages

2021 ◽  
Author(s):  
Giulia Maria Pires dos Santos ◽  
Gustavo Ramalho Cardoso dos Santos ◽  
Mariana Ingrid Dutra da Silva Xisto ◽  
Rodrigo Rollin-Pinheiro ◽  
Andréa Regina de Souza Baptista ◽  
...  
Keyword(s):  
Cell Wall ◽  
Yeast Cell ◽  
Chemical Structure ◽  
Histoplasma Capsulatum ◽  
Peritoneal Macrophages ◽  
Yeast Cell Wall
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