scholarly journals A20 regulates inflammation through autophagy mediated by NF-κB pathway in human nucleus pulposus cells and ameliorates disc degeneration in vivo

2021 ◽  
Vol 549 ◽  
pp. 179-186
Author(s):  
Ye Zhang ◽  
Weiwei Yi ◽  
Huiqiang Xia ◽  
Haiyang Lan ◽  
Jie Chen ◽  
...  
Keyword(s):  
Nucleus Pulposus ◽  
Disc Degeneration ◽  
Nucleus Pulposus Cells

Baicalein Inhibits the IL-1β-Induced Inflammatory Response in Nucleus Pulposus Cells and Attenuates Disc Degeneration In vivo

Inflammation ◽  
2019 ◽  
Vol 42 (3) ◽  
pp. 1032-1044 ◽  
Author(s):  
Haiming Jin ◽  
Qingqing Wang ◽  
Jianwei Wu ◽  
Xuyao Han ◽  
Tianchen Qian ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Nucleus Pulposus ◽  
Disc Degeneration ◽  
Nucleus Pulposus Cells

scholarly journals Metformin protects against apoptosis and senescence in nucleus pulposus cells and ameliorates disc degeneration in vivo

2016 ◽  
Vol 7 (10) ◽  
pp. e2441-e2441 ◽  
Author(s):  
Deheng Chen ◽  
Dongdong Xia ◽  
Zongyou Pan ◽  
Daoliang Xu ◽  
Yifei Zhou ◽  
...  
Keyword(s):  
Nucleus Pulposus ◽  
Disc Degeneration ◽  
Nucleus Pulposus Cells

Therapeutic Potential of Naringin for Intervertebral Disc Degeneration: Involvement of Autophagy Against Oxidative Stress-Induced Apoptosis in Nucleus Pulposus Cells

2018 ◽  
Vol 46 (07) ◽  
pp. 1561-1580 ◽  
Author(s):  
Zengjie Zhang ◽  
Chenggui Wang ◽  
Jialiang Lin ◽  
Haiming Jin ◽  
Ke Wang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Intervertebral Disc ◽  
Nucleus Pulposus ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Therapeutic Potential ◽  
Nucleus Pulposus Cells ◽  
Induced Apoptosis

Intervertebral disc degeneration (IDD) is a major cause of lower back pain, but few efficacious medicines have been developed for IDD. Increased nucleus pulposus cells apoptosis is a dominant pathogenesis of IDD and is considered a therapeutic target. Previously, our group proved that autophagy may protect nucleus pulposus cells against apoptosis. As one of the major bioflavonoids of citrus, naringin activates autophagy. Therefore, we hypothesize that naringin may have therapeutic potential for IDD by activating autophagy in nucleus pulposus cells. In this study, we evaluated the effects of naringin on TBHP-induced oxidative stress in nucleus pulposus cells in vitro as well as in puncture-induced rat IDD model in vivo. Our results showed that naringin could reduce the incidence of oxidative stress-induced apoptosis in nucleus pulposus cells and promoted the expression of autophagy markers LC3-II/I and beclin-1. Meanwhile, inhibition of autophagy by 3-MA may partially reverse the anti-apoptotic effect of naringin, indicating that autophagy was involved in the protective effect of naringin in nucleus pulposus cells. Further study showed that autophagy regulation of naringin may be related to AMPK signaling. Also, we found that naringin treatment can regulate the expression of collagen II, aggrecan and Mmp13 to sustain the extracellular matrix. Furthermore, our in vivo study showed that naringin can ameliorate IDD in puncture-induced rat model. In conclusion, our study suggests that naringin can protect nucleus pulposus cells against apoptosis and ameliorate IDD in vivo, the mechanism may relate to its autophagy regulation.

LncRNA MALAT1 exhibits positive effects on nucleus pulposus cells biology in vivo and in vitro by sponging miR-503 media the P38MAPKs/AP-1 pathway

2019 ◽  
Author(s):  
Hongyu Zheng ◽  
Tingting Wang ◽  
Xiangmin Li ◽  
Wei He ◽  
Zhiqiang Gong ◽  
...  
Keyword(s):  
Nucleus Pulposus ◽  
Disc Degeneration ◽  
Mapk Pathway ◽  
Critical Role ◽  
Molecular Study ◽  
Mapk Signaling ◽  
Nucleus Pulposus Cells ◽  
Positive Effects

Abstract Background: Intervertebral disc degeneration (IDD) is characterized by the loss of nucleus pulposus cells (NPCs) and phenotypic abnormalities. Accumulated evidence suggests that long non-coding RNAs (lncRNAs) are involved in the pathogenesis of IDD. In this study, we aimed to investigate the functional effects of lncMALAT1 on NPCs in IDD and the possible mechanism. Results: We validated the decreased expression of MALAT1 in the IDD tissues, associating with increased collagen II and aggrecan expression. In vitro, overexpressed MALAT1 could attenuate the effect of IL-1β on NPCs proliferation, apoptosis, and aggrecan degradation. In vivo, MALAT1 overexpression attenuated the severity of disc degeneration in IDD model rats. Our molecular study further demonstrated that MALAT1 could sponge miR-503, modulate the expression of miR-503, and the activation of downstream MAPK signaling pathways. The effects of MALAT1 on NPCs were partially reversed/ aggregated by miR-503 mimics/inhibitor treatment. Conclusion: Our data suggested that MALAT1-miR-503-MAPK pathway plays a critical role in NPCs, which may be a potential strategy for improving IDD.

Transplantation of Activated Nucleus Pulposus Cells after Cryopreservation: Efficacy Study in Canine Disc Degeneration Model

2014 ◽  
Vol 4 (1_suppl) ◽  
pp. s-0034-1376573-s-0034-1376573
Author(s):  
T. Nukaga ◽  
D. Sakai ◽  
A. Hiyama ◽  
T. Ishii ◽  
T. Nakai ◽  
...  
Keyword(s):  
Nucleus Pulposus ◽  
Disc Degeneration ◽  
Efficacy Study ◽  
Nucleus Pulposus Cells
Sign in / Sign up

Export Citation Format

Share Document