Chemical inhibitor anticancer drugs regulate mechanical properties and cytoskeletal structure of non-invasive and invasive breast cancer cell lines: Study of effects of Letrozole, Exemestane, and Everolimus

2021 ◽  
Vol 565 ◽  
pp. 14-20
Author(s):  
Ehsan Mohammadi ◽  
Mohammad Tabatabaei ◽  
Mahdi Habibi-Anbouhi ◽  
Mohammad Tafazzoli-Shadpour
Keyword(s):  
Breast Cancer ◽  
Mechanical Properties ◽  
Cell Lines ◽  
Anticancer Drugs ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Invasive Breast Cancer ◽  
Breast Cancer Cell Lines ◽  
Non Invasive ◽  
Cytoskeletal Structure

scholarly journals Fibronectin Affects Transient MMP2 Gene Expression through DNA Demethylation Changes in Non-Invasive Breast Cancer Cell Lines

PLoS ONE ◽  
2014 ◽  
Vol 9 (9) ◽  
pp. e105806 ◽  
Author(s):  
Isabela T. Pereira ◽  
Edneia A. S. Ramos ◽  
Erico T. Costa ◽  
Anamaria A. Camargo ◽  
Graciele C. M. Manica ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Invasive Breast Cancer ◽  
Dna Demethylation ◽  
Breast Cancer Cell Lines ◽  
Invasive Breast Cancer Cell ◽  
Non Invasive

Abstract 5410: Simultaneously tackling NFκB and JNK pathways to sensitise breast cancer cell lines to conventional anticancer drugs

2010 ◽  
Author(s):  
Nga C. Yip* ◽  
Ivo S. Fombon* ◽  
Peng Liu ◽  
Sarah Brown ◽  
Angel L. Armesilla ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Lines ◽  
Anticancer Drugs ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines

scholarly journals Investigation on the Composition of Agarose–Collagen I Blended Hydrogels as Matrices for the Growth of Spheroids from Breast Cancer Cell Lines

Pharmaceutics ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 963
Author(s):  
Alessandra Quarta ◽  
Nunzia Gallo ◽  
Daniele Vergara ◽  
Luca Salvatore ◽  
Concetta Nobile ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Mechanical Properties ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Physical And Mechanical Properties ◽  
Cancer Cell Lines ◽  
Collagen I ◽  
Breast Cancer Cell Lines ◽  
Culture Systems

Three-dimensional (3D) cell culture systems mimic the structural complexity of the tissue microenvironment and are gaining increasing importance as they resemble the extracellular matrix (ECM)–cell and cell–cell physical interactions occurring in vivo. Several scaffold-based culture systems have been already proposed as valuable tools for large-scale production of spheroids, but they often suffer of poor reproducibility or high costs of production. In this work, we present a reliable 3D culture system based on collagen I-blended agarose hydrogels and show how the variation in the agarose percentage affects the physical and mechanical properties of the resulting hydrogel. The influence of the different physical and mechanical properties of the blended hydrogels on the growth, size, morphology, and cell motility of the spheroids obtained by culturing three different breast cancer cell lines (MCF-7, MDA-MB-361, and MDA-MB-231) was also evaluated. As proof of concept, the cisplatin penetration and its cytotoxic effect on the tumor spheroids as function of the hydrogel stiffness were also investigated. Noteworthily, the possibility to recover the spheroids from the hydrogels for further processing and other biological studies has been considered. This feature, in addition to the ease of preparation, the lack of cross-linking chemistry and the high reproducibility, makes this hydrogel a reliable biomimetic matrix for the growth of 3D cell structures.

scholarly journals Agarose-collagen I hydrogels: impact of the matrix stiffness on the growth of breast cancer cell lines spheroids and on drug penetration

2021 ◽  
Author(s):  
ALESSANDRA QUARTA ◽  
Nunzia Gallo ◽  
Daniele Vergara ◽  
Luca Salvatore ◽  
Concetta Nobile ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Mechanical Properties ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
3D Culture ◽  
Physical And Mechanical Properties ◽  
Collagen I ◽  
Breast Cancer Cell Lines ◽  
Culture Systems

Three-dimensional (3D) cell culture systems mimic the structural complexity of the tissue microenvironment that includes the extracellular matrix (ECM) in addition to the cellular components Thus, 3D culture systems are increasingly important as they resemble the ECM-cell and cell-cell physical interactions occurring in vivo. So far, several scaffold-based culture systems and techniques have been proposed as valuable approaches for large-scale production of spheroids, but often suffering of poor reproducible conditions or high costs of production. In this work we present a reliable 3D culture system based on collagen I-blended agarose hydrogels and show how the variation of the agarose weight percentage affects the physical and mechanical properties of the resulting hydrogel, being that with a lower amount of agarose more permeable, softer and more prone to degradation compared to hydrogels with higher agarose concentrations. We have also evaluated the effect of the different physical and mechanical properties of the agarose hydrogels on the growth, size, morphology and cell motility of spheroids obtained by culturing three different breast cancer cell lines (MCF-7, MDA-MB-361and MDA-MB-231). As proof of concept, the cisplatin penetration and its cytotoxic effect on the tumor spheroids was evaluated as function of the hydrogel stiffness. Noteworthy, the possibility to recover the spheroids from the hydrogels for further processing and other biological studies has been also considered.

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