Rhodotorula glutinis cultivation on cassava wastewater for carotenoids and fatty acids generation

2019 ◽  
Vol 22 ◽  
pp. 101419 ◽  
Author(s):  
José Evangelista Santos Ribeiro ◽  
Amanda Marília da Silva Sant'Ana ◽  
Mina Martini ◽  
Carlo Sorce ◽  
Andrea Andreucci ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Rhodotorula Glutinis ◽  
Cassava Wastewater

scholarly journals Fatty Acids Effect on Lipase and Biosurfactant Induction in Rhodotorula Glutinis CMGB-RG5

2018 ◽  
Vol 1 (1) ◽  
pp. 515-522 ◽  
Author(s):  
Ortansa Csutak ◽  
Viorica Corbu ◽  
Ileana Stoica ◽  
Tatiana Vassu
Keyword(s):  
Fatty Acids ◽  
Antimicrobial Activity ◽  
Olive Oil ◽  
Rhodotorula Glutinis ◽  
Tween 80 ◽  
Screening Tests ◽  
Tween 20 ◽  
Emulsification Index ◽  
Carbon Substrates ◽  
Microbial Lipases

AbstractFatty acids represent important substrates for obtaining microbial lipases and biosurfactants. The yeast strain CMGBRG5 was identified as Rhodotorula glutinis using the BIOLOG MicroLog System. The opacity screening tests showed that R. glutinis CMGB-RG5 was able to produce lipases in presence of 1% Tween 80 after three days of incubation. Lipase induction was estimated as cell growth in presence of Tween 80, Tween 20, olive oil and tributyrin. After 48 hours, best results were obtained in presence of butyric acid, respectively, oleic acid: 2.2 × 107cells/ml on tributyrin and 1.0 × 107cells/ml on olive oil. Biosurfactant production was evaluated as emulsification index (E24%). After one week, high E24 values were obtained on fried sunflower oil (53%) and olive oil (35%). Crude and concentrated biosurfactants were tested against nine Candida strains. Best antimicrobial activity was observed for [20X] biosurfactants against C. tropicalis, C. guilliermondii and C. krusei. In conclusion, R. glutinis CGB-RG5 shows high potential for using fatty acids from various sources as unique carbon substrates for synthesis of biocompounds with high economic and biotechnological value.

Purification and characterization of acyl-acyl carrier protein synthetase from oleaginous yeast and its role in triacylglycerol biosynthesis

2001 ◽  
Vol 360 (2) ◽  
pp. 471-479 ◽  
Author(s):  
Akanksha GANGAR ◽  
Anjali A. KARANDE ◽  
Ram RAJASEKHARAN
Keyword(s):  
Fatty Acids ◽  
Oleaginous Yeast ◽  
Acyl Carrier Protein ◽  
Rhodotorula Glutinis ◽  
Cross Reactivity ◽  
Carrier Protein ◽  
Dependent Manner ◽  
Triacylglycerol Biosynthesis ◽  
Reducing Conditions ◽  
Apparent Homogeneity

Fatty acids are activated in an ATP-dependent manner before they are utilized. We describe here how the 10S triacylglycerol biosynthetic multienzyme complex from Rhodotorula glutinis is capable of activating non-esterified fatty acids for the synthesis of triacylglycerol. The photolabelling of the complex with [32P]azido-ATP showed labelling of a 35kDa polypeptide. The labelled polypeptide was identified as acyl-acyl carrier protein (ACP) synthetase, which catalyses the ATP-dependent ligation of fatty acid with ACP to form acyl-ACP. The enzyme was purified by successive PAGE separations to apparent homogeneity from the soluble fraction of oleaginous yeast and its apparent molecular mass was 35kDa under denaturing and reducing conditions. Acyl-ACP synthetase was specific for ATP. The Km values for palmitic, stearic, oleic and linoleic acids were found to be 42.9, 30.4, 25.1 and 22.7μM, respectively. The antibodies to acyl-ACP synthetase cross-reacted with Escherichia coli acyl-ACP synthetase. Anti-ACP antibodies showed no cross-reactivity with the purified acyl-ACP synthetase, indicating no bound ACP with the enzyme. Immunoprecipitations with antibodies to acyl-ACP synthetase revealed that this enzyme is a part of the 10S triacylglycerol biosynthetic complex. These results demonstrate that the soluble acyl-ACP synthetase plays a novel role in activating fatty acids for triacylglycerol biosynthesis in oleaginous yeast.

Selective recovery and purification of carotenoids and fatty acids from Rhodotorula glutinis using mixtures of biosolvents

2021 ◽  
Vol 266 ◽  
pp. 118548
Author(s):  
Cassamo U. Mussagy ◽  
Daniela Remonatto ◽  
Ariela V. Paula ◽  
Rondinelli D. Herculano ◽  
Valéria C. Santos-Ebinuma ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Rhodotorula Glutinis ◽  
Selective Recovery

scholarly journals Effect of cassava wastewater on physicochemical characteristics and fatty acids composition of meat from feedlot-finished lambs

2017 ◽  
Vol 39 (4) ◽  
pp. 377
Author(s):  
Jose Adelson Santana Neto ◽  
Evandro Neves Muniz ◽  
Gladston Rafael Arruda Santos ◽  
Francisco de Assis Fonseca Macedo ◽  
Jose Henrique De Albuquerque Rangel ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Physicochemical Characteristics ◽  
Fatty Acids Composition ◽  
Cassava Wastewater

Acidogenic fermentation of cassava wastewater for volatile fatty acids production

2020 ◽  
Vol 25 (2) ◽  
pp. 245
Author(s):  
Mirian Yasmine Krauspenhar Niz ◽  
Edinéia Lazarotto Formagini ◽  
Marc Àrpad Boncz ◽  
Paula Loureiro Paulo
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Acidogenic Fermentation ◽  
Cassava Wastewater

Biotechnology as a useful tool for nutritional improvement of cereal-based materials enriched with polyunsaturated fatty acids and pigments

2008 ◽  
Vol 56 (4) ◽  
pp. 377-384 ◽  
Author(s):  
M. čertík ◽  
Z. Adamechová ◽  
V. Hanusová ◽  
E. Breierová
Keyword(s):  
Fatty Acids ◽  
Arachidonic Acid ◽  
Solid State ◽  
Polyunsaturated Fatty Acids ◽  
Eicosapentaenoic Acid ◽  
Linolenic Acid ◽  
Rhodotorula Glutinis ◽  
Mortierella Alpina ◽  
Nutritional Improvement ◽  
Sporobolomyces Roseus

The fungi Thamnidium elegans , as a producer of γ-linolenic acid (GLA), and Mortierella alpina , as a producer of dihomo-γ-linolenic acid (DGLA), arachidonic acid (AA) and eicosapentaenoic acid (EPA), and the yeasts Rhodotorula glutinis and Sporobolomyces roseus , as producers of ß-carotene, torulene and torularhodin, were tested for their ability to utilize cereal substrates during solid state fermentations (SSF). Depending on the strain and conditions, the cereal materials were effectively enriched with polyunsaturated fatty acids (PUFAs) or carotenoids. These naturally prepared bioproducts could find applications in food, feed, biomedical, pharmaceutical and veterinary fields.

scholarly journals Physicochemical composition and fatty acids profile of the meat of lambs fed with cassava wastewater

2019 ◽  
Vol 9 (4) ◽  
pp. 456-555 ◽  
Author(s):  
Stela Antas Urbano ◽  
Marcelo Andrade Ferreira ◽  
Paulo Sérgio Azevedo ◽  
Gustavo Araújo Vasconcelos ◽  
Hilson Barreto Santos Filho ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Shear Force ◽  
Monounsaturated Fatty Acids ◽  
Quadratic Effect ◽  
Linear Effect ◽  
Physicochemical Composition ◽  
Fatty Acids Profile ◽  
Cassava Wastewater ◽  
Breed Sheep ◽  
Minimum Force

The effects of replacing corn with cassava wastewater at 0; 25; 50; 75 and 100% in the sheep diet over chemical composition, fatty acid profile and physical-chemical parameters of meat were studied. Forty Santa Inês breed sheep were slaughtered after 70 days of confinement. The moisture content of meat increased, while the crude protein and ether extract decreased linearly with the replacement. There was a quadratic effect for shear force with minimum force estimated at 2.78 kg / cm ² with 54.25% replacement. There was a quadratic effect for C15:0 and C:17, (0.37 and 1.30 when the replacement was 46.25% and 68.50%, respectively), increasing linearly for the C18:0 and decreasing for the C22:0. For monounsaturated fatty acids there was a decreasing linear effect for C14:1 and C20:1 n9, and increasing to C18:1 n11. For C15:1 a quadratic effect was observed (1.48 when 26.75% of corn was replaced). As to the polyunsaturated fatty acids, there was a quadratic effect for C18:2 9c 11t (0.60 when replacement was 36.66%). There was a linear decrease for relations polyunsaturated: saturated and ω6: ω3, without effect on other relations. Cassava wastewater alters the shear force and chemical and lipid composition of meat.

Acidogenic fermentation of cassava wastewater for volatile fatty acids production

2020 ◽  
Vol 25 (2) ◽  
pp. 245
Author(s):  
Edinéia Lazarotto Formagini ◽  
Paula Loureiro Paulo ◽  
Marc Àrpad Boncz ◽  
Mirian Yasmine Krauspenhar Niz
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Acidogenic Fermentation ◽  
Cassava Wastewater
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