Selective recovery and purification of carotenoids and fatty acids from Rhodotorula glutinis using mixtures of biosolvents

AbstractFatty acids represent important substrates for obtaining microbial lipases and biosurfactants. The yeast strain CMGBRG5 was identified as Rhodotorula glutinis using the BIOLOG MicroLog System. The opacity screening tests showed that R. glutinis CMGB-RG5 was able to produce lipases in presence of 1% Tween 80 after three days of incubation. Lipase induction was estimated as cell growth in presence of Tween 80, Tween 20, olive oil and tributyrin. After 48 hours, best results were obtained in presence of butyric acid, respectively, oleic acid: 2.2 × 107cells/ml on tributyrin and 1.0 × 107cells/ml on olive oil. Biosurfactant production was evaluated as emulsification index (E24%). After one week, high E24 values were obtained on fried sunflower oil (53%) and olive oil (35%). Crude and concentrated biosurfactants were tested against nine Candida strains. Best antimicrobial activity was observed for [20X] biosurfactants against C. tropicalis, C. guilliermondii and C. krusei. In conclusion, R. glutinis CGB-RG5 shows high potential for using fatty acids from various sources as unique carbon substrates for synthesis of biocompounds with high economic and biotechnological value.

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Carotenoids and Fatty Acids in Red Yeasts Sporobolomyces roseus and Rhodotorula glutinis

Applied Biochemistry and Microbiology ◽

10.1023/b:abim.0000033917.57177.f2 ◽

2004 ◽

Vol 40 (4) ◽

pp. 392-397 ◽

Cited By ~ 64

Author(s):

P. Davoli ◽

V. Mierau ◽

R. W. S. Weber

Keyword(s):

Fatty Acids ◽

Rhodotorula Glutinis ◽

Sporobolomyces Roseus

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Purification and characterization of acyl-acyl carrier protein synthetase from oleaginous yeast and its role in triacylglycerol biosynthesis

Biochemical Journal ◽

10.1042/bj3600471 ◽

2001 ◽

Vol 360 (2) ◽

pp. 471-479 ◽

Cited By ~ 8

Author(s):

Akanksha GANGAR ◽

Anjali A. KARANDE ◽

Ram RAJASEKHARAN

Keyword(s):

Fatty Acids ◽

Oleaginous Yeast ◽

Acyl Carrier Protein ◽

Rhodotorula Glutinis ◽

Cross Reactivity ◽

Carrier Protein ◽

Dependent Manner ◽

Triacylglycerol Biosynthesis ◽

Reducing Conditions ◽

Apparent Homogeneity

Fatty acids are activated in an ATP-dependent manner before they are utilized. We describe here how the 10S triacylglycerol biosynthetic multienzyme complex from Rhodotorula glutinis is capable of activating non-esterified fatty acids for the synthesis of triacylglycerol. The photolabelling of the complex with [32P]azido-ATP showed labelling of a 35kDa polypeptide. The labelled polypeptide was identified as acyl-acyl carrier protein (ACP) synthetase, which catalyses the ATP-dependent ligation of fatty acid with ACP to form acyl-ACP. The enzyme was purified by successive PAGE separations to apparent homogeneity from the soluble fraction of oleaginous yeast and its apparent molecular mass was 35kDa under denaturing and reducing conditions. Acyl-ACP synthetase was specific for ATP. The Km values for palmitic, stearic, oleic and linoleic acids were found to be 42.9, 30.4, 25.1 and 22.7μM, respectively. The antibodies to acyl-ACP synthetase cross-reacted with Escherichia coli acyl-ACP synthetase. Anti-ACP antibodies showed no cross-reactivity with the purified acyl-ACP synthetase, indicating no bound ACP with the enzyme. Immunoprecipitations with antibodies to acyl-ACP synthetase revealed that this enzyme is a part of the 10S triacylglycerol biosynthetic complex. These results demonstrate that the soluble acyl-ACP synthetase plays a novel role in activating fatty acids for triacylglycerol biosynthesis in oleaginous yeast.

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Biotechnology as a useful tool for nutritional improvement of cereal-based materials enriched with polyunsaturated fatty acids and pigments

Acta Agronomica Hungarica ◽

10.1556/aagr.56.2008.4.1 ◽

2008 ◽

Vol 56 (4) ◽

pp. 377-384 ◽

Cited By ~ 6

Author(s):

M. čertík ◽

Z. Adamechová ◽

V. Hanusová ◽

E. Breierová

Keyword(s):

Fatty Acids ◽

Arachidonic Acid ◽

Solid State ◽

Polyunsaturated Fatty Acids ◽

Eicosapentaenoic Acid ◽

Linolenic Acid ◽

Rhodotorula Glutinis ◽

Mortierella Alpina ◽

Nutritional Improvement ◽

Sporobolomyces Roseus

The fungi Thamnidium elegans , as a producer of γ-linolenic acid (GLA), and Mortierella alpina , as a producer of dihomo-γ-linolenic acid (DGLA), arachidonic acid (AA) and eicosapentaenoic acid (EPA), and the yeasts Rhodotorula glutinis and Sporobolomyces roseus , as producers of ß-carotene, torulene and torularhodin, were tested for their ability to utilize cereal substrates during solid state fermentations (SSF). Depending on the strain and conditions, the cereal materials were effectively enriched with polyunsaturated fatty acids (PUFAs) or carotenoids. These naturally prepared bioproducts could find applications in food, feed, biomedical, pharmaceutical and veterinary fields.

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