Development of fed-batch process for high-yielding β-glucosidase displayed on cell surface of industrial yeast Saccharomyces cerevisiae

2017 ◽  
Vol 128 ◽  
pp. 195-200 ◽  
Author(s):  
Kaoru Onodera ◽  
Shinji Hama ◽  
Ayumi Yoshida ◽  
Hideo Noda ◽  
Akihiko Kondo
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Surface ◽  
Batch Process ◽  
Industrial Yeast ◽  
Fed Batch ◽  
Yeast Saccharomyces Cerevisiae

scholarly journals Cell surface recycling in yeast: mechanisms and machineries

2016 ◽  
Vol 44 (2) ◽  
pp. 474-478 ◽  
Author(s):  
Chris MacDonald ◽  
Robert C. Piper
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Plasma Membrane ◽  
Membrane Protein ◽  
Cell Surface ◽  
Mammalian Cells ◽  
Genetic System ◽  
Integral Membrane Protein ◽  
Protein Activity ◽  
Yeast Saccharomyces Cerevisiae ◽  
Central Process

Sorting internalized proteins and lipids back to the cell surface controls the supply of molecules throughout the cell and regulates integral membrane protein activity at the surface. One central process in mammalian cells is the transit of cargo from endosomes back to the plasma membrane (PM) directly, along a route that bypasses retrograde movement to the Golgi. Despite recognition of this pathway for decades we are only beginning to understand the machinery controlling this overall process. The budding yeast Saccharomyces cerevisiae, a stalwart genetic system, has been routinely used to identify fundamental proteins and their modes of action in conserved trafficking pathways. However, the study of cell surface recycling from endosomes in yeast is hampered by difficulties that obscure visualization of the pathway. Here we briefly discuss how recycling is likely a more prevalent process in yeast than is widely appreciated and how tools might be built to better study the pathway.

Lipid Rafts in Protein Sorting and Cell Polarity in Budding Yeast Saccharomyces cerevisiae

2002 ◽  
Vol 383 (10) ◽  
pp. 1475-1480 ◽  
Author(s):  
M. Bagnat ◽  
K. Simons
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Surface ◽  
Lipid Rafts ◽  
Cell Polarity ◽  
Budding Yeast ◽  
Protein Sorting ◽  
Yeast Saccharomyces Cerevisiae ◽  
Functional Consequences ◽  
Acyl Chains

Abstract Cellular membranes contain many types and species of lipids. One of the most important functional consequences of this heterogeneity is the existence of microdomains within the plane of the membrane. Sphingolipid acyl chains have the ability of forming tightly packed platforms together with sterols. These platforms or lipid rafts constitute segregation and sorting devices into which proteins specifically associate. In budding yeast, Saccharomyces cerevisiae, lipid rafts serve as sorting platforms for proteins destined to the cell surface. The segregation capacity of rafts also provides the basis for the polarization of proteins at the cell surface during mating. Here we discuss some recent findings that stress the role of lipid rafts as key players in yeast protein sorting and cell polarity.

Improving ethanol production and viability of Saccharomyces cerevisiae by a vitamin feeding strategy during fed-batch process

2002 ◽  
Vol 60 (1-2) ◽  
pp. 67-72 ◽  
Author(s):  
Alfenore S. ◽  
Molina-Jouve C. ◽  
Guillouet S. ◽  
Uribelarrea J.-L. ◽  
Goma G. ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Production ◽  
Feeding Strategy ◽  
Batch Process ◽  
Fed Batch
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