Statistical optimization of aqueous ammonia pretreatment and enzymatic hydrolysis of corn cob powder for enhancing sugars production

Effects of soaking aqueous ammonia pretreatment on chemical composition and enzymatic hydrolysis of corn stover. The aim of this research was to investigate the effect of applying two different temperatures of the soaking aqueous ammonia treatment on the chemical composition and enzymatic hydrolysis yield of the corn stover. Native corn stover as well as solid fractions after 20 h of alkali pretreatment performed at 15% ammonia solution and at 50 °C or 90 °C were analysed in terms of cellulose, holocellulose, lignin and extractives content. Both untreated and treated samples were subjected to the enzymatic hydrolysis and hydrolysates were examined with a high performance liquid chromatography (HPLC). Results indicated a significant development of enzymatic digestibility of the SAA treated biomass. Furthermore, a 38.7% and a 68.9% delignification levels in the biomass treated with ammonia at respectively 50 °C and 90 °C process comparing to the raw material were achieved.

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Enhanced enzymatic hydrolysis of corncob by ultrasound-assisted soaking in aqueous ammonia pretreatment

3 Biotech ◽

10.1007/s13205-018-1186-2 ◽

2018 ◽

Vol 8 (3) ◽

Cited By ~ 7

Author(s):

Ruoyu Du ◽

Rongxin Su ◽

Wei Qi ◽

Zhimin He

Keyword(s):

Enzymatic Hydrolysis ◽

Aqueous Ammonia ◽

Ultrasound Assisted ◽

Ammonia Pretreatment ◽

Hydrolysis Of ◽

Soaking In Aqueous Ammonia

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Enhancing enzymatic hydrolysis of corn cob, corn stover and sorghum stalk by dilute aqueous ammonia combined with ultrasonic pretreatment

Industrial Crops and Products ◽

10.1016/j.indcrop.2017.08.038 ◽

2017 ◽

Vol 109 ◽

pp. 220-226 ◽

Cited By ~ 33

Author(s):

Qin-Qin Xu ◽

Meng-Jiao Zhao ◽

Zhen-Zi Yu ◽

Jian-Zhong Yin ◽

Guo-Min Li ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Corn Stover ◽

Aqueous Ammonia ◽

Corn Cob ◽

Ultrasonic Pretreatment ◽

Hydrolysis Of

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Improving the efficiency of enzymatic hydrolysis of Eucalyptus residues with a modified aqueous ammonia soaking method

Nordic Pulp & Paper Research Journal ◽

10.1515/npprj-2018-3025 ◽

2018 ◽

Vol 33 (2) ◽

pp. 165-174 ◽

Cited By ~ 2

Author(s):

Dan Huo ◽

Qiulin Yang ◽

Guigan Fang ◽

Qiujuan Liu ◽

Chuanling Si ◽

...

Keyword(s):

Hydrogen Peroxide ◽

Enzymatic Hydrolysis ◽

Aqueous Ammonia ◽

Pulp Mill ◽

Fiber Surface ◽

Raw Material ◽

Sem Images ◽

Aqueous Ammonia Soaking ◽

Guaiacyl Lignin ◽

Hydrolysis Of

Abstract Eucalyptus residues from pulp mill were pretreated with aqueous ammonia soaking (AAS) method to improve the efficiency of enzymatic hydrolysis. The optimized condition of AAS was obtained by response surface methodology. Meanwhile, hydrogen peroxide was introduced into the AAS system to modify the AAS pretreatment (AASP). The results showed that a fermentable sugar yield of 64.96 % was obtained when the eucalypt fibers were pretreated at the optimal conditions, with 80 % of ammonia (w/w) for 11 h and keeping the temperature at 90 °C. In further research it was found that the addition of H2O2 to the AAS could improve the pretreatment efficiency. The delignification rate and enzymatic digestibility were increased to 64.49 % and 73.85 %, respectively, with 5 % of hydrogen peroxide being used. FTIR analysis indicated that most syringyl and guaiacyl lignin and a trace amount of xylan were degraded and dissolved during the AAS and AASP pretreatments. The CrI of the raw material was increased after AAS and AASP pretreatments, which was attributed to the removal of amorphous portion. SEM images showed that microfibers were separated and explored from the initial fiber structure after AAS pretreatment, and the AASP method could improve the destructiveness of the fiber surface.

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