Cell surface display of Neurospora crassa glutamate decarboxylase on Escherichia coli for extracellular Gamma-aminobutyric acid production from high cell density culture

2021 ◽  
Vol 176 ◽  
pp. 108196
Author(s):  
Sivachandiran Somasundaram ◽  
Jaehoon Jeong ◽  
Soon Ho Hong
Keyword(s):  
Escherichia Coli ◽  
Cell Surface ◽  
Glutamate Decarboxylase ◽  
High Cell Density ◽  
Surface Display ◽  
Cell Surface Display ◽  
Aminobutyric Acid ◽  
High Cell Density Culture ◽  
Gamma Aminobutyric Acid ◽  
High Cell

scholarly journals Designing novel construction for cell surface display of protein E on Escherichia coli using non-classical pathway based on Lpp-OmpA

AMB Express ◽  
2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Meisam Jeiranikhameneh ◽  
Mohamad Reza Razavi ◽  
Shiva Irani ◽  
Seyed Davar Siadat ◽  
Mana Oloomi
Keyword(s):  
Escherichia Coli ◽  
Cell Surface ◽  
Surface Display ◽  
Cell Surface Display ◽  
Classical Pathway

scholarly journals Functional Cell Surface Display and Controlled Secretion of Diverse Agarolytic Enzymes by Escherichia coli with a Novel Ligation-Independent Cloning Vector Based on the Autotransporter YfaL

2012 ◽  
Vol 78 (9) ◽  
pp. 3051-3058 ◽  
Author(s):  
Hyeok-Jin Ko ◽  
Eunhye Park ◽  
Joseph Song ◽  
Taek Ho Yang ◽  
Hee Jong Lee ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell Surface ◽  
Fluorescent Protein ◽  
Surface Display ◽  
Cell Surface Display ◽  
Heterologous Proteins ◽  
Display System ◽  
Reporter Protein ◽  
Content Type ◽  
Ligation Independent Cloning

ABSTRACTAutotransporters have been employed as the anchoring scaffold for cell surface display by replacing their passenger domains with heterologous proteins to be displayed. We adopted an autotransporter (YfaL) ofEscherichia colifor the cell surface display system. The critical regions in YfaL for surface display were identified for the construction of a ligation-independent cloning (LIC)-based display system. The designed system showed no detrimental effect on either the growth of the host cell or overexpressing heterologous proteins on the cell surface. We functionally displayed monomeric red fluorescent protein (mRFP1) as a reporter protein and diverse agarolytic enzymes fromSaccharophagus degradans2-40, including Aga86C and Aga86E, which previously had failed to be functional expressed. The system could display different sizes of proteins ranging from 25.3 to 143 kDa. We also attempted controlled release of the displayed proteins by incorporating a tobacco etch virus protease cleavage site into the C termini of the displayed proteins. The maximum level of the displayed protein was 6.1 × 104molecules per a single cell, which corresponds to 5.6% of the entire cell surface of actively growingE. coli.

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