Reducing the cost for cellulase production by improving efficiency to prepare the soluble inducer and saccharification of corn stover using in-house generated crude enzymes

2021 ◽  
pp. 108296
Author(s):  
Yonghao Li ◽  
Peng Zhang ◽  
Deying Zhu ◽  
Bo Yao ◽  
Tomohisa Hasunuma ◽  
...  
1969 ◽  
Vol 16 (3) ◽  
Author(s):  
Anuj K Chandel ◽  
Om V Singh ◽  
Gajula Chandrasekhar ◽  
Linga Venkateswar Rao ◽  
Mangamoori Lakshmi Narasu

The imposition of ethanol derived from biomass for blending in gasoline would make countries less dependent on current petroleum sources, which would save foreign exchange reserves, improve rural economies and provide job opportunities in a clean and safe environment. The key drivers for successful commercial ethanol production are cheap raw materials, economic pretreatment technologies, in-house cellulase production with high and efficient titers, high ethanol fermentation rates, downstream recovery of ethanol and maximum by-products utilization. Furthermore, recent developments in engineering of biomass for increased biomass, down-regulation of lignin synthesis, improved cellulase titers and re-engineering of cellulases, and process integration of the steps involved have increased the possibility of cheap bioethanol production that competes with the price of petroleum. Recently, many companies have come forward globally for bioethanol production on a large scale. It is very clear now that bioethanol will be available at the price of fossil fuels by 2010. This article intends to provide insight and perspectives on the important recent developments in bioethanol research, the commercialization status of bioethanol production, the step-wise cost incurred in the process involved, and the possible innovations that can be utilized to reduce the cost of ethanol production.


2021 ◽  
Author(s):  
Genilton S Faheina ◽  
Kally A Sousa ◽  
Jerri E Zilli ◽  
Carlos Vergara ◽  
Gustavo A. Saavedra Pinto ◽  
...  

Abstract Cellulases are a complex of enzymes necessary for the complete solubilization of cellulose in sugars, thus playing a key role in the natural carbon cycle through the hydrolysis of lignocellulosic structures. The aim of this study was to evaluate the increase in the capacity of Talaromyces amestolkiae CMIAT 055 to produce cellulases by optimizing the components of the culture medium containing banana pseudostem as an inducer, as well as in different agitation configurations in a bioreactor. Optimization was performed through statistical experimental design (Plackett-Burman and DCCR), a study of pH control in bioreactors, and a study of the agitation system by comparing impellers with different flow profiles in the liquid medium. For this purpose, a wild strain of Talaromyces amestolkiae CMIAT 055 was used. In the Plackett-Burman and DCCR statistical design, four components of the culture medium were significant and optimized for greater synthesis of FPase: banana pseudostem, CaCl2, KH2PO4, and urea. In bioreactors tests, these parameters were beneficial for greater enzyme activities: maintenance of pH at 5.0, use of Pitched blade impeller, and rotation speed at 300 rpm. Comparing the first test using banana pseudostem in an Erlenmeyer flask to the last fermentation process in bioreactors, it was observed that the total cellulase activity increased from 424.7 FPU/L to 2172.8 FPU/L. This fact showed that the strategies adopted in this study are a pertinent way to reduce the cost of enzyme production through the use of lignocellulosic materials.


2015 ◽  
Vol 18 (01) ◽  
pp. 213-217 ◽  
Author(s):  
Zhaoli Meng ◽  
Qiuhong Xie ◽  
Yang Liu ◽  
Xinyue Zhang ◽  
Hongyu Xiang

2021 ◽  
Vol 9 ◽  
Author(s):  
William L. Kubic ◽  
Cameron M. Moore ◽  
Troy A. Semelsberger ◽  
Andrew D. Sutton

Converting biomass into jet fuel involves more than the core chemical process. The overall process includes the logistics of harvesting and transporting the biomass, handling and preparing the material for processing, and processing and disposal of waste. All of these activities contribute to cost. Controlling cost involves more than developing efficient process chemistry. Choice of feedstock also has a significant impact on process economics. We consider chemical conversion of paper from municipal solid waste as a feedstock for the production of jet fuel and diesel. Paper has a significantly higher cellulose content than raw lignocellulosic biomass such as corn stover, so it requires less pretreatment to convert it into hydrocarbons than lignocellulosic biomass. Our techno-economic analysis showed that the cost of converting paper waste into jet fuel is about $1.00/gal less than jet fuel produced from corn stover. Although the cost of recycling paper into jet fuel is less than producing it from corn stover, the process is not competitive with petroleum. We estimated a minimum selling price of $3.97/gal for paper-derived jet fuel. Our sensitivity studies indicated that the biggest economic obstacle is the cost of cellulose hydrolysis. Direct hydrogenation of paper to sugar alcohols combined with increased economy of scale could make recycling paper jet fuel competitive.


BioResources ◽  
2020 ◽  
Vol 15 (3) ◽  
pp. 6974-6988
Author(s):  
Rui-Ping Xi ◽  
Ye-Qiang Qi ◽  
Bi-Xian Zhang ◽  
Xin-Miao He ◽  
He-Shu Chen ◽  
...  

Ionic liquids (ILs) are effective solvents for lignocellulose pretreatment. Enzymatic saccharification converts pretreated lignocelluloses into valuable products, and IL-tolerant cellulase improves the enzymatic efficiency and the reuse of ILs. In this study, a fungal strain with a relatively high cellulase production was isolated and identified as Aspergillus aculeatus G1-3. The high production of β-glucosidase (1.943 U per mL), CMCase (1.303 U per mL), and FPase (0.165 U per mL) was obtained using corn stover as the carbon source and peptone as the nitrogen source. The results were obtained at pH 8.0 and 30 °C with an inoculation size of 3% (volume per volume) for 7 days. A mutant strain Aspergillus aculeatus P6 with β-glucosidase (7.023 U per mL), CMCase (1.543 U per mL), and FPase (0.098 U per mL) was obtained by 60Co-γ irradiation. The cellulase activity was measured at pH 5.0 and 60 °C for enzymatic hydrolysis. The cellulase from mutant strains was stable in different concentrations of 1-ethyl-3-methylimidazolium acetate. Enzymatic saccharification of the original corn stover and ILs-pretreated corn stover was successfully performed with high sugar yields. The mutant strains of Aspergillus aculeatus have great potential for their further application in the conversion of lignocellulosic biomass into biofuels.


RSC Advances ◽  
2017 ◽  
Vol 7 (89) ◽  
pp. 56239-56246 ◽  
Author(s):  
Chen Zhao ◽  
Lu Deng ◽  
Hao Fang ◽  
Shaolin Chen

Mixed culture ofTrichoderma reeseiandAspergillus nigerwas employed to accomplish on-site cellulase production where cellulases were applied directly to the enzymatic hydrolysis of pretreated corn stover.


Energy ◽  
2017 ◽  
Vol 123 ◽  
pp. 499-510 ◽  
Author(s):  
Chen Zhao ◽  
Zongsheng Zou ◽  
Jisheng Li ◽  
Honglei Jia ◽  
Johannes Liesche ◽  
...  

2018 ◽  
Vol 118 ◽  
pp. 14-24 ◽  
Author(s):  
Chen Zhao ◽  
Zongsheng Zou ◽  
Jisheng Li ◽  
Honglei Jia ◽  
Johannes Liesche ◽  
...  

RSC Advances ◽  
2013 ◽  
Vol 3 (48) ◽  
pp. 25960 ◽  
Author(s):  
Alan Culbertson ◽  
Mingjie Jin ◽  
Leonardo da Costa Sousa ◽  
Bruce E. Dale ◽  
Venkatesh Balan

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