Exploratory behaviour in the open field test adapted for larval zebrafish: Impact of environmental complexity

2013 ◽  
Vol 92 ◽  
pp. 88-98 ◽  
Author(s):  
Farooq Ahmad ◽  
Michael K. Richardson
Keyword(s):  
Open Field ◽  
Field Test ◽  
Open Field Test ◽  
Exploratory Behaviour ◽  
Environmental Complexity ◽  
Larval Zebrafish

scholarly journals The Open Field Test

2021 ◽  
Author(s):  
Vootele Voikar ◽  
s. Clare Stanford
Keyword(s):  
Locomotor Activity ◽  
Open Field ◽  
Field Test ◽  
Open Field Test ◽  
Exploratory Behaviour ◽  
Biological Factors ◽  
Laboratory Conditions ◽  
General Locomotor Activity ◽  
Rats And Mice

The Open Field Test (OFT) is the most widely used method for observing the behaviour of mice and rats 18 under laboratory conditions. It is used to evaluate the exploratory behaviour, general locomotor activity 19 and emotionality of rats and mice. However, it has to be kept in mind that these behaviours are not 20 independent, they interact and so a change in one will affect another. Despite being a seemingly simple 21 test, several caveats need to be acknowledged when selecting the variables for analysis and interpreting 22 the data with regard to the aforementioned domains. The observed behaviours can depend on a number of 23 procedural, environmental and biological factors, which should be carefully considered when planning the 24 experiment, as well as during the analysis and interpretation of the results. This review provides a critical 25 overview of these factors, followed by some warnings and practical tips for conducting the OFT.

162. THE EFFECT OF MATERNAL FOLIC ACID SUPPLEMENTATION THROUGHOUT PREGNANCY ON NEURODEVELOPMENT, MOTOR FUNCTION AND BEHAVIOUR OF PROGENY IN THE RAT

2009 ◽  
Vol 21 (9) ◽  
pp. 80
Author(s):  
B. L. Pearce ◽  
J. A. Owens ◽  
M. Dziadek ◽  
P. A. Grant ◽  
M. Wlodek ◽  
...  
Keyword(s):  
Folic Acid ◽  
Open Field ◽  
Motor Function ◽  
Field Test ◽  
Open Field Test ◽  
Folic Acid Supplementation ◽  
Exploratory Behaviour ◽  
Acid Supplementation ◽  
Righting Reflex ◽  
Eye Opening

Background: Maternal folic acid supplementation (mFAS) during early pregnancy is recommended to reduce the incidence of neural tube defects and has recently been associated with improved neurodevelopment in children. However, the effect on neurodevelopment of mFAS from before conception and throughout pregnancy is unknown. We examined the effect of mFAS throughout the gestational period on postnatal growth, neurodevelopment and early adult motor function and behaviour in rat offspring. Methods: Female Wistar Rats were fed either a control (folic acid 2mg/kg, n=6) or moderate mFAS diet (folic acid 6mg/kg, n=6) from two weeks before mating with Lewis males, until birth of progeny. Male and female progeny (Control=36, mFAS=36) were weighed on postnatal day (PD) 3, 7, 14, 21, 40 and 90, and underwent various tests between PD4 and 14: righting reflex, palm-grasp reflex, negative geotaxis, forelimb hanging, ascent test and eye opening. Locomotor/ exploratory behaviour, motor coordination and anxiety were assessed using an open field test (PD52), rotarod (PD55) and elevated plus maze (PD58) (Control=24, mFAS=24). Results: mFAS did not alter maternal weight gain, litter-size at birth or progeny growth between PD3-90. mFAS tended to increase righting reflex time (p=0.057) and impair ascent ability (p=0.085). Negative geotaxis time was reduced at PD7 but not later (Diet x Age p=0.051). mFAS increased the proportion of progeny with eyes open at PD14 (p=0.008) and tended to increase forelimb hanging time(p=0.097). mFAS did not alter motor learning/function (rotarod), but increased ambulatory and exploratory behaviour (open field test; p=0.027). Conclusions: mFAS delays some early aspects of neurodevelopment including neonatal postural reflex maturation and proprioceptive/vestibular function, but accelerates others such as eye opening. However, the open field test indicated that mFAS improved the offspring's locomotion and exploratory behaviours in adulthood. Further studies will differentiate the neurodevelopmental effects of mFAS around conception from gestation-long mFAS.

scholarly journals Toxicity and Neuropharmacological Effects of Elenine

2011 ◽  
Vol 2011 ◽  
pp. 1-10 ◽  
Author(s):  
Eduardo Navarro ◽  
S. J. Alonso ◽  
R. Navarro
Keyword(s):  
Open Field ◽  
Field Test ◽  
Chemical Structure ◽  
Open Field Test ◽  
Control Group ◽  
General Behaviour ◽  
Similar Chemical ◽  
Sedative Effects ◽  
Traction Test ◽  
Central Depressant

Elenine is the aglycone of elenoside, a cytotoxic arylnaphthalene lignan (NSC 644013-W/1) derived fromJusticia hyssopifolia. (Family: Acanthaceae). Elenoside is a β-D-glucoside, with a similar chemical structure to etoposide, exhibiting central depressant activity. In the present study, elenine was given to mice and rats at doses of 10, 20, and 40 mg/kg. Acute toxicity (24 h) and general behaviour in mice was studied as well as its effects on muscular relaxant activity, locomotor activity (Varimex test), and the open-field test and were compared with 10 mg/kg of chlorpromazine. Elenine produced a reduction in the permanence time in muscular relaxant activity (traction test). Spontaneous activity was lower in the Varimex test. The ambulation and rearing were lower compared with the control group, and an increase in boluses was observed in the open-field test. Thus, it can be concluded that elenine has central sedative effects at lower doses than those used with elenoside and has a possible application in conditions of anxiety.

scholarly journals Participation ofGABAAChloride Channels in the Anxiolytic-Like Effects of a Fatty Acid Mixture

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Juan Francisco Rodríguez-Landa ◽  
Rosa Isela García-Ríos ◽  
Jonathan Cueto-Escobedo ◽  
Blandina Bernal-Morales ◽  
Carlos M. Contreras
Keyword(s):  
Open Field ◽  
Field Test ◽  
Chloride Channels ◽  
Wistar Rats ◽  
Elevated Plus Maze ◽  
Open Field Test ◽  
Acid Mixture ◽  
Plus Maze ◽  
Gaba Binding ◽  
Defensive Burying

Human amniotic fluid and a mixture of eight fatty acids (FAT-M) identified in this maternal fluid (C12:0, lauric acid, 0.9 μg%; C14:0, myristic acid, 6.9 μg%; C16:0, palmitic acid, 35.3 μg%; C16:1, palmitoleic acid, 16.4 μg%; C18:0, stearic acid, 8.5 μg%; C18:1cis, oleic acid, 18.4 μg%; C18:1trans, elaidic acid, 3.5 μg%; C18:2, linoleic acid, 10.1 μg%) produce anxiolytic-like effects that are comparable to diazepam in Wistar rats, suggesting the involvement ofγ-aminobutyric acid-A (GABAA) receptors, a possibility not yet explored. Wistar rats were subjected to the defensive burying test, elevated plus maze, and open field test. In different groups, threeGABAAreceptor antagonists were administered 30 min before FAT-M administration, including the competitive GABA binding antagonist bicuculline (1 mg/kg),GABAAbenzodiazepine antagonist flumazenil (5 mg/kg), and noncompetitiveGABAAchloride channel antagonist picrotoxin (1 mg/kg). The FAT-M exerted anxiolytic-like effects in the defensive burying test and elevated plus maze, without affecting locomotor activity in the open field test. TheGABAAantagonists alone did not produce significant changes in the behavioral tests. Picrotoxin but not bicuculline or flumazenil blocked the anxiolytic-like effect of the FAT-M. Based on the specific blocking action of picrotoxin on the effects of the FAT-M, we conclude that the FAT-M exerted its anxiolytic-like effects throughGABAAreceptor chloride channels.

Acute effects of ethanol on behavioral responses of male and female zebrafish in the open field test with the influence of a non-familiar object

2021 ◽  
pp. 104474
Author(s):  
Thiele P. Souza ◽  
Francini Franscescon ◽  
Flavia V. Stefanello ◽  
Talise E. Müller ◽  
Laura W. Santos ◽  
...  
Keyword(s):  
Open Field ◽  
Field Test ◽  
Open Field Test ◽  
Behavioral Responses ◽  
Familiar Object ◽  
Acute Effects ◽  
Male And Female ◽  
Female Zebrafish

Garcinia Camobogia Extract Alters Anxiety, Sociability, and Dopamine Turnover in Male Swiss Albino Mice

2021 ◽  
Author(s):  
Michael K. Ibrahim ◽  
Marina Aboelsaad ◽  
Fatma Tony ◽  
Moustafa Sayed
Keyword(s):  
Open Field ◽  
Field Test ◽  
Open Field Test ◽  
Modern Medicine ◽  
Receptor Expression ◽  
Dopamine Turnover ◽  
Male Mice ◽  
Garcinia Cambogia ◽  
Swiss Albino Mice ◽  
Albino Mice

Abstract Background: Obesity is a global concern, closely allied with somatic and psychosomatic disorders. Herbal drugs are available in modern medicine to treat obesity. Garcinia camobogia being used by so many people trying to lose weight produces various systemic side effects. The study was conducted to assess its effect on anxiety, sociability, and dopamine turnover in male mice. Methodology: Male Swiss albino mice of either were divided into three groups with seven mice in each group. Different groups were given distilled water (0.5ml p.o.) and Garcinia cambogia extract at two different doses (100mg/kg and 500 mg/kg p.o.). Effect of test drugs on anxiety was evaluated using open field test. Sociability and social novelty were evaluated using three chambers test. Results (mean ± standard deviation) were analyzed using one-way ANOVA test followed by Tukey’s test. Result: Garcinia cambogia extract significantly increased the time spent in the corners in the open field test, significantly reduced sociability and social novelty in the three chamber test, significantly reduced dopamine turnover and increased D2 receptor expression in ventral tegmental area. Conclusion: Garcinia cambogia extract have significant anxiogenic effect along with reduced sociability and social novelty in male mice. Moreover, these effects could be related to the altered dopamine turnover and D2 receptor expression in mice brain.

Early deprivation increases high-leaning behavior, a novel anxiety-like behavior, in the open field test in rats

2017 ◽  
Vol 123 ◽  
pp. 27-35 ◽  
Author(s):  
Hiroshi Kuniishi ◽  
Satoshi Ichisaka ◽  
Miki Yamamoto ◽  
Natsuko Ikubo ◽  
Sae Matsuda ◽  
...  
Keyword(s):  
Open Field ◽  
Field Test ◽  
Open Field Test ◽  
Early Deprivation
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