Analysis of expressed sequence tags from the venom ducts of Conus striatus: focusing on the expression profile of conotoxins

Biochimie ◽  
2006 ◽  
Vol 88 (2) ◽  
pp. 131-140 ◽  
Author(s):  
Canhui Pi ◽  
Yun Liu ◽  
Can Peng ◽  
Xiuhua Jiang ◽  
Junliang Liu ◽  
...  
Keyword(s):  
Expression Profile ◽  
Expressed Sequence Tags ◽  
Expressed Sequence ◽  
Conus Striatus

Isolation, identification and expression profile of fourteen differentially expressed sequence tags in the longissimus dorsi muscle tissues from Meishan, Meishan×Large White cross and Large White pigs

2007 ◽  
Vol 4 (1) ◽  
pp. 9-14
Author(s):  
Liu Yong-Gang ◽  
Xiong Yuan-Zhu ◽  
Zuo Bo ◽  
Jiang Si-Wen ◽  
Deng Chang-Yan ◽  
...  
Keyword(s):  
Expression Profile ◽  
Differential Display ◽  
Expressed Sequence Tags ◽  
Differentially Expressed ◽  
Longissimus Dorsi ◽  
Large White ◽  
Longissimus Dorsi Muscle ◽  
Dorsi Muscle ◽  
Muscle Tissues ◽  
Expressed Sequence

AbstractIn order to detect the molecular mechanism of heterosis in pigs, an mRNA differential display (DD) technique was performed to investigate the differences in gene expression in the longissimus dorsi muscle tissues from Meishan, Meishan×Large White cross and Large White pigs. Fourteen expressed sequence tags (ESTs), differentially expressed between the hybrid and purebred pigs, were isolated and identified through semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Nucleotide sequence analysis revealed that the 14 ESTs are not homologous to any of the known genes or ESTs. These novel ESTs were then deposited in the GenBank database. Tissue expression profile analysis showed that the ESTs were expressed in most tissues, including heart, spleen, liver, kidney, small intestine, ovary and lung, and this also implied that these genes must be important for the life process. Our results indicate the diversity of differential display of genes between the hybrids and purebreds in the Meishan×Large White cross combination. Results also suggest that heterosis in pigs might be derived from the differential expression of many indispensable genes in specific life phases.

scholarly journals Generation and chromosome mapping of expressed sequence tags (ESTs) from a human infant thymus

Genome ◽  
1999 ◽  
Vol 42 (3) ◽  
pp. 457-464 ◽  
Author(s):  
Mee-Yul Hwang ◽  
Yoon-Joong Kang ◽  
Young-Ho Kim ◽  
Steve W Scherer ◽  
Lap-Chee Tsui ◽  
...  
Keyword(s):  
Expression Profile ◽  
Radiation Hybrid ◽  
Expressed Sequence Tags ◽  
Radiation Hybrid Mapping ◽  
Human Infant ◽  
Hybrid Mapping ◽  
Novel Genes ◽  
Ehlers Danlos Syndrome ◽  
Disease Loci ◽  
Expressed Sequence

In an effort to identify novel genes that are expressed differentially in an infant thymus, we constructed an oligo-d(T) primed cDNA library from a human infant thymus followed by single-run partial sequencing to generate expressed sequence tags (ESTs). Characterization of more than 1400 sequences enabled us to convert human thymus transcripts into 1223 useful ESTs. These ESTs consisted of 613 (50.1%) showing homology to known human genes, 51 (4.2%) matching to genes from other species, 289 (23.6%) matching ESTs of unknown functions, and 182 (14.9%) being novel transcripts. The expression profile of an infant thymus features a high number of genes related to cell division-DNA synthesis and gene-protein expression, indicating the active growth stage of an infant thymus. To identify the chromosomal localization of 43 thymus ESTs, PCR-based mapping was performed using a human-rodent somatic cell hybrid or radiation hybrid mapping panel. The results indicated that several novel genes were determined to be located in the vicinity of previously mapped disease loci; histidinemia loci, plasminogen Tochigi disease loci, Ehlers-Danlos syndrome, hypertriglyceridemia, thyroid resistance locus, ocular albinism, galactosemia, porphyria variegata, Charcot-Marie-tooth disease, FEOM (fibrosis of extraocular muscles), Prader-Willi syndrome.Key words: cDNA, expression profile, radiation hybrid mapping, disease locus.

scholarly journals Gene Expression Profile in the Anterior Regeneration of the Earthworm Using Expressed Sequence Tags

2009 ◽  
Vol 73 (1) ◽  
pp. 29-34 ◽  
Author(s):  
Sung-Jin CHO ◽  
Myung Sik LEE ◽  
Eun Sik TAK ◽  
Eun LEE ◽  
Ki Seok KOH ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Expressed Sequence Tags ◽  
Expressed Sequence ◽  
Anterior Regeneration

scholarly journals Gene Discovery and Expression Profile Analysis through Sequencing of Expressed Sequence Tags from Different Developmental Stages of the Chytridiomycete Blastocladiella emersonii

2005 ◽  
Vol 4 (2) ◽  
pp. 455-464 ◽  
Author(s):  
Karina F. Ribichich ◽  
Silvia M. Salem-Izacc ◽  
Raphaela C. Georg ◽  
Ricardo Z. N. Vêncio ◽  
Luci D. Navarro ◽  
...  
Keyword(s):  
Life Cycle ◽  
Expression Profile ◽  
Expressed Sequence Tags ◽  
Developmental Stages ◽  
Profile Analysis ◽  
Protein Biosynthesis ◽  
Northern Analysis ◽  
Cdna Clones ◽  
Blastocladiella Emersonii ◽  
Expressed Sequence

ABSTRACT Blastocladiella emersonii is an aquatic fungus of the chytridiomycete class which diverged early from the fungal lineage and is notable for the morphogenetic processes which occur during its life cycle. Its particular taxonomic position makes this fungus an interesting system to be considered when investigating phylogenetic relationships and studying the biology of lower fungi. To contribute to the understanding of the complexity of the B. emersonii genome, we present here a survey of expressed sequence tags (ESTs) from various stages of the fungal development. Nearly 20,000 cDNA clones from 10 different libraries were partially sequenced from their 5′ end, yielding 16,984 high-quality ESTs. These ESTs were assembled into 4,873 putative transcripts, of which 48% presented no matches with existing sequences in public databases. As a result of Gene Ontology (GO) project annotation, 1,680 ESTs (35%) were classified into biological processes of the GO structure, with transcription and RNA processing, protein biosynthesis, and transport as prevalent processes. We also report full-length sequences, useful for construction of molecular phylogenies, and several ESTs that showed high similarity with known proteins, some of which were not previously described in fungi. Furthermore, we analyzed the expression profile (digital Northern analysis) of each transcript throughout the life cycle of the fungus using Bayesian statistics. The in silico approach was validated by Northern blot analysis with good agreement between the two methodologies.

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