Insight into the reaction mechanism of the Escherichia coli cyclopropane fatty acid synthase: Isotope exchange and kinetic isotope effects

Biochimie ◽

10.1016/j.biochi.2010.05.019 ◽

2010 ◽

Vol 92 (10) ◽

pp. 1454-1457 ◽

Author(s):

Guangqi E ◽

Denis Lesage ◽

Olivier Ploux

Keyword(s):

Escherichia Coli ◽

Reaction Mechanism ◽

Isotope Exchange ◽

Fatty Acid Synthase ◽

Isotope Effects ◽

Kinetic Isotope Effects ◽

Cyclopropane Fatty Acid ◽

Kinetic Isotope ◽

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Reactivity and Secondary Kinetic Isotope Effects in the SN2 Reaction Mechanism: Dioxygen Radical Anion and Related Nucleophiles

Journal of the American Chemical Society ◽

10.1021/ja993093x ◽

2000 ◽

Vol 122 (8) ◽

pp. 1740-1748 ◽

Author(s):

Gustavo E. Davico ◽

Veronica M. Bierbaum

Keyword(s):

Reaction Mechanism ◽

Radical Anion ◽

Isotope Effects ◽

Kinetic Isotope Effects ◽

Kinetic Isotope ◽

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Bromine kinetic isotope effects: insight into Grignard reagent formation

New Journal of Chemistry ◽

10.1039/c3nj00197k ◽

2013 ◽

Vol 37 (8) ◽

pp. 2241 ◽

Author(s):

Lukasz Szatkowski ◽

Agnieszka Dybala-Defratyka ◽

Charlie Batarseh ◽

Jochanan Blum ◽

Ludwik Halicz ◽

...

Keyword(s):

Grignard Reagent ◽

Isotope Effects ◽

Kinetic Isotope Effects ◽

Kinetic Isotope ◽

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Permeable membrane/mass spectrometric measurement of solvent proton/deuterium, carbon-12/carbon-13, and oxygen-16/oxygen-18 kinetic isotope effects associated with .alpha.-chymotrypsin deacylation: evidence for reaction mechanism plasticity

Biochemistry ◽

10.1021/bi00371a014 ◽

1986 ◽

Vol 25 (23) ◽

pp. 7328-7336 ◽

Author(s):

Awadhesh K. Mishra ◽

Michael H. Klapper

Keyword(s):

Reaction Mechanism ◽

Isotope Effects ◽

Kinetic Isotope Effects ◽

Mass Spectrometric ◽

Permeable Membrane ◽

Mass Spectrometric Measurement ◽

Spectrometric Measurement ◽

Kinetic Isotope ◽

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Cyclopropane Fatty Acid Synthase from Escherichia coli: Enzyme Purification and Inhibition by Vinylfluorine and Epoxide-Containing Substrate Analogues

ChemBioChem ◽

10.1002/cbic.200300767 ◽

2003 ◽

Vol 4 (12) ◽

pp. 1352-1356 ◽

Author(s):

Erich J. Molitor ◽

Beth M. Paschal ◽

Hung-wen Liu

Keyword(s):

Escherichia Coli ◽

Enzyme Purification ◽

Fatty Acid Synthase ◽

Cyclopropane Fatty Acid ◽

Substrate Analogues

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Acidity of hydrocarbons. XLVII. Primary kinetic isotope effects and internal return in hydrogen isotope exchange of toluene and triphenylmethane with lithium cyclohexylamide

Journal of the American Chemical Society ◽

10.1021/ja00794a022 ◽

1973 ◽

Vol 95 (13) ◽

pp. 4254-4257 ◽

Author(s):

A. Streitwieser ◽

P. H. Owens ◽

G. Sonnichsen ◽

W. K. Smith ◽

G. R. Ziegler ◽

...

Keyword(s):

Hydrogen Isotope ◽

Isotope Exchange ◽

Isotope Effects ◽

Kinetic Isotope Effects ◽

Hydrogen Isotope Exchange ◽

Kinetic Isotope

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Transcriptional and Translational Leakiness in Expression of a Fungal Cyclopropane Fatty Acid Synthase in the Escherichia coli pET-vector Expression System

Current Trends in Biotechnology and Pharmacy ◽

10.5530/ctbp.2021.1.1 ◽

2020 ◽

Vol 15 (1) ◽

pp. 1-14

Author(s):

Ursula Kües ◽

Sevda Haghi Kia ◽

Kiran Lakkireddy ◽

Mojtaba Zomorrodi

Keyword(s):

Escherichia Coli ◽

Fatty Acid Synthase ◽

Expression System ◽

Cyclopropane Fatty Acid

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Ni2+-activated glyoxalase I from Escherichia coli: Substrate specificity, kinetic isotope effects and evolution within the βαβββ superfamily

Journal of Inorganic Biochemistry ◽

10.1016/j.jinorgbio.2011.11.008 ◽

2012 ◽

Vol 108 ◽

pp. 133-140 ◽

Author(s):

Kadia Y. Mullings ◽

Nicole Sukdeo ◽

Uthaiwan Suttisansanee ◽

Yanhong Ran ◽

John F. Honek

Keyword(s):

Escherichia Coli ◽

Substrate Specificity ◽

Isotope Effects ◽

Kinetic Isotope Effects ◽

Glyoxalase I ◽

Kinetic Isotope

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Interpretation of the Gas-Phase Solvent Deuterium Kinetic Isotope Effects in the SN2 Reaction Mechanism: Comparison of Theoretical and Experimental Results in the Reaction of Microsolvated Fluoride Ions with Methyl Halides

The Journal of Physical Chemistry A ◽

10.1021/jp0627168 ◽

2006 ◽

Vol 110 (48) ◽

pp. 13112-13121 ◽

Author(s):

Gustavo E. Davico

Keyword(s):

Reaction Mechanism ◽

Gas Phase ◽

Isotope Effects ◽

Kinetic Isotope Effects ◽

Experimental Results ◽

Fluoride Ions ◽

Methyl Halides ◽

Kinetic Isotope ◽

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Cyclopropane fatty acid synthase of Escherichia coli: Deduced amino acid sequence, purification, and studies of the enzyme active site

Biochemistry ◽

10.1021/bi00160a011 ◽

1992 ◽

Vol 31 (45) ◽

pp. 11020-11028 ◽

Author(s):

Ai Yu Wang ◽

Dennis W. Grogan ◽

John E. Cronan

Keyword(s):

Escherichia Coli ◽

Amino Acid Sequence ◽

Active Site ◽

Fatty Acid Synthase ◽

Cyclopropane Fatty Acid ◽

Enzyme Active Site

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Functional characterization of a stereospecific diol dehydrogenase, FucO, from Escherichia coli: Substrate specificity, pH dependence, kinetic isotope effects and influence of solvent viscosity

Journal of Molecular Catalysis B Enzymatic ◽

10.1016/j.molcatb.2010.04.010 ◽

2010 ◽

Vol 66 (1-2) ◽

pp. 148-155 ◽

Author(s):

Cecilia Blikstad ◽

Mikael Widersten

Keyword(s):

Escherichia Coli ◽

Substrate Specificity ◽

Isotope Effects ◽

Ph Dependence ◽

Functional Characterization ◽

Kinetic Isotope Effects ◽

Solvent Viscosity ◽

Kinetic Isotope ◽

Influence Of Solvent

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