Transcriptional and Translational Leakiness in Expression of a Fungal Cyclopropane Fatty Acid Synthase in the Escherichia coli pET-vector Expression System

Ursula Kües; Sevda Haghi Kia; Kiran Lakkireddy; Mojtaba Zomorrodi

doi:10.5530/ctbp.2021.1.1

Transcriptional and Translational Leakiness in Expression of a Fungal Cyclopropane Fatty Acid Synthase in the Escherichia coli pET-vector Expression System

Current Trends in Biotechnology and Pharmacy ◽

10.5530/ctbp.2021.1.1 ◽

2020 ◽

Vol 15 (1) ◽

pp. 1-14

Author(s):

Ursula Kües ◽

Sevda Haghi Kia ◽

Kiran Lakkireddy ◽

Mojtaba Zomorrodi

Keyword(s):

Escherichia Coli ◽

Fatty Acid ◽

Fatty Acid Synthase ◽

Expression System ◽

Cyclopropane Fatty Acid

Download Full-text

Cyclopropane Fatty Acid Synthase from Escherichia coli: Enzyme Purification and Inhibition by Vinylfluorine and Epoxide-Containing Substrate Analogues

ChemBioChem ◽

10.1002/cbic.200300767 ◽

2003 ◽

Vol 4 (12) ◽

pp. 1352-1356 ◽

Cited By ~ 9

Author(s):

Erich J. Molitor ◽

Beth M. Paschal ◽

Hung-wen Liu

Keyword(s):

Escherichia Coli ◽

Fatty Acid ◽

Enzyme Purification ◽

Fatty Acid Synthase ◽

Cyclopropane Fatty Acid ◽

Substrate Analogues

Download Full-text

Cyclopropane fatty acid synthase of Escherichia coli: Deduced amino acid sequence, purification, and studies of the enzyme active site

Biochemistry ◽

10.1021/bi00160a011 ◽

1992 ◽

Vol 31 (45) ◽

pp. 11020-11028 ◽

Cited By ~ 62

Author(s):

Ai Yu Wang ◽

Dennis W. Grogan ◽

John E. Cronan

Keyword(s):

Escherichia Coli ◽

Fatty Acid ◽

Amino Acid ◽

Amino Acid Sequence ◽

Active Site ◽

Fatty Acid Synthase ◽

Cyclopropane Fatty Acid ◽

Enzyme Active Site

Download Full-text

Cyclopropane fatty acid synthase of Escherichia coli. Stabilization, purification, and interaction with phospholipid vesicles

Biochemistry ◽

10.1021/bi00582a015 ◽

1979 ◽

Vol 18 (15) ◽

pp. 3292-3300 ◽

Cited By ~ 42

Author(s):

Frederick R. Taylor ◽

John E. Cronan

Keyword(s):

Escherichia Coli ◽

Fatty Acid ◽

Fatty Acid Synthase ◽

Phospholipid Vesicles ◽

Cyclopropane Fatty Acid

Download Full-text

Cloning and manipulation of the Escherichia coli cyclopropane fatty acid synthase gene: physiological aspects of enzyme overproduction.

Journal of Bacteriology ◽

10.1128/jb.158.1.286-295.1984 ◽

1984 ◽

Vol 158 (1) ◽

pp. 286-295 ◽

Cited By ~ 45

Author(s):

D W Grogan ◽

J E Cronan

Keyword(s):

Escherichia Coli ◽

Fatty Acid ◽

Fatty Acid Synthase ◽

Cyclopropane Fatty Acid ◽

Enzyme Overproduction ◽

Fatty Acid Synthase Gene

Download Full-text

[18] Cyclopropane fatty acid synthase from Escherichia coli

Methods in Enzymology - Lipids Part C ◽

10.1016/0076-6879(81)71020-6 ◽

1981 ◽

pp. 133-139 ◽

Cited By ~ 11

Author(s):

Frederick R. Taylor ◽

Dennis W. Grogan ◽

John E. Cronan

Keyword(s):

Escherichia Coli ◽

Fatty Acid ◽

Fatty Acid Synthase ◽

Cyclopropane Fatty Acid

Download Full-text

Insight into the reaction mechanism of the Escherichia coli cyclopropane fatty acid synthase: Isotope exchange and kinetic isotope effects

Biochimie ◽

10.1016/j.biochi.2010.05.019 ◽

2010 ◽

Vol 92 (10) ◽

pp. 1454-1457 ◽

Cited By ~ 8

Author(s):

Guangqi E ◽

Denis Lesage ◽

Olivier Ploux

Keyword(s):

Escherichia Coli ◽

Fatty Acid ◽

Reaction Mechanism ◽

Isotope Exchange ◽

Fatty Acid Synthase ◽

Isotope Effects ◽

Kinetic Isotope Effects ◽

Cyclopropane Fatty Acid ◽

Kinetic Isotope ◽

Insight Into

Download Full-text

An active site mutant of Escherichia coli cyclopropane fatty acid synthase forms new non-natural fatty acids providing insights on the mechanism of the enzymatic reaction

Biochimie ◽

10.1016/j.biochi.2013.08.007 ◽

2013 ◽

Vol 95 (12) ◽

pp. 2336-2344 ◽

Cited By ~ 4

Author(s):

Guangqi E ◽

Thierry Drujon ◽

Isabelle Correia ◽

Olivier Ploux ◽

Dominique Guianvarc'h

Keyword(s):

Escherichia Coli ◽

Fatty Acids ◽

Fatty Acid ◽

Active Site ◽

Fatty Acid Synthase ◽

Enzymatic Reaction ◽

Cyclopropane Fatty Acid

Download Full-text

Escherichia coli cyclopropane fatty acid synthase. Mechanistic and site-directed mutagenetic studies

European Journal of Biochemistry ◽

10.1111/j.1432-1033.2004.04441.x ◽

2004 ◽

Vol 271 (23-24) ◽

pp. 4769-4778 ◽

Cited By ~ 16

Author(s):

Fabienne Courtois ◽

Christine Guerard ◽

Xavier Thomas ◽

Olivier Ploux

Keyword(s):

Escherichia Coli ◽

Fatty Acid ◽

Fatty Acid Synthase ◽

Cyclopropane Fatty Acid

Download Full-text

Characterization of recombinant thioesterase and acyl carrier protein domains of chicken fatty acid synthase expressed in Escherichia coli

Journal of Biological Chemistry ◽

10.1016/s0021-9258(19)84696-3 ◽

1989 ◽

Vol 264 (30) ◽

pp. 18195-18201

Author(s):

M Pazirandeh ◽

S S Chirala ◽

W Y Huang ◽

S J Wakil

Keyword(s):

Escherichia Coli ◽

Fatty Acid ◽

Fatty Acid Synthase ◽

Acyl Carrier Protein ◽

Protein Domains ◽

Carrier Protein

Download Full-text

Site-directed mutagenesis studies on the recombinant thioesterase domain of chicken fatty acid synthase expressed in Escherichia coli.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)54802-x ◽

1991 ◽

Vol 266 (31) ◽

pp. 20946-20952

Author(s):

M. Pazirandeh ◽

S.S. Chirala ◽

S.J. Wakil

Keyword(s):

Escherichia Coli ◽

Fatty Acid ◽

Fatty Acid Synthase ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis

Download Full-text