scholarly journals Extreme homozygosity in Southern Hemisphere populations of Deladenus siricidicola, a biological control agent of Sirex noctilio

2011 ◽  
Vol 59 (3) ◽  
pp. 348-353 ◽  
Author(s):  
X. Osmond Mlonyeni ◽  
Brenda D. Wingfield ◽  
Michael J. Wingfield ◽  
Rodrigo Ahumada ◽  
Paula Klasmer ◽  
...  
2012 ◽  
Vol 42 (6) ◽  
pp. 1173-1177 ◽  
Author(s):  
I. Leal ◽  
B. Foord ◽  
C. Davis ◽  
P. de Groot ◽  
X.O. Mlonyeni ◽  
...  

The woodwasp Sirex noctilio Fabricius, along with its obligate symbiotic fungus Amylostereum areolatum (Chaillet ex Fr.) Boidin, is amongst the most damaging invasive species to many commercial pine plantations. The most effective biocontrol agent for management of this woodwasp has been the nematode Deladenus siricidicola Bedding. Before this agent can be used in North America, answering key questions about its interaction with native siricids and other strains of the nematode is essential, as would be the need to track its spread after release. The aim of this study was to develop tools to differentiate between the North American D. siricidicola isolates and the Southern Hemisphere Kamona strain of this species. We sequenced a region from ribosomal DNA and the cytochrome oxidase subunit 1 and developed a PCR–RFLP method based on a single nucleotide polymorphism flanking a microsatellite sequence. These markers will be useful for science-based operational biocontrol of S. noctilio.


NeoBiota ◽  
2019 ◽  
Vol 44 ◽  
pp. 39-55
Author(s):  
Tonya D. Bittner ◽  
Nathan Havill ◽  
Isis A.L. Caetano ◽  
Ann E. Hajek

Sirexnoctilio is an invasive woodwasp that, along with its symbiotic fungus, has killed pine trees (Pinus spp.) in North America and in numerous countries in the Southern Hemisphere. We tested a biological control agent in North America that has successfully controlled S.noctilio in Oceania, South Africa, and South America. Deladenussiricidicola nematodes feed on the symbiotic white rot fungus Amylostereumareolatum and can switch to being parasitic on S.noctilio. When parasitic, the Kamona nematode strain can sterilise the eggs of S.noctilio females. However, in North America, a different strain of D.siricidicola (NA), presumably introduced along with the woodwasp, parasitises but does not sterilise S.noctilio. We tested the sterilising Kamona biological control strain of D.siricidicola against S.noctilio in North America. Interactions between the biological control strain and the NA strain could include competitive exclusion, co-infection within hosts or hybridisation. We reared D.siricidicola Kamona on an A.areolatum strain native to North America (IGS-BE) and another strain (IGS-BDF) used commercially to mass-produce the nematode in Australia. We inoculated Kamona reared on either strain of A.areolatum into logs infested with S.noctilio larvae and compared parasitism rates against control logs. Individual nematodes were isolated from S.noctilio hemocoels and from sterilised eggs and were genotyped with eight microsatellite loci. A high rate of parasitisation of S.noctilio by D.siricidicolaNA was found for all treatments and we found evidence of both co-infection and hybridisation. Surprisingly, sterilisation rates were not related to the rates of parasitisation by D.siricidicola Kamona.


2007 ◽  
Vol 24 (3) ◽  
pp. 165-167 ◽  
Author(s):  
Kevin J. Dodds ◽  
Robert R. Cooke ◽  
Daniel W. Gilmore

Abstract A nonnative woodwasp of Eurasian origin, Sirex noctilio F., was detected recently in Oswego, New York, infesting Scots, red, and white pine. S. noctilio has caused periodic widespread losses of pine timber resources in several Southern Hemisphere countries and may cause significant damage in pure even-aged stands and overstocked plantations in North America. However, stand management and biological control programs have successfully managed S. noctilio populations in other countries and similar programs are being developed for North America. Until the primary biological control agent, Beddingia siricidicola, a parasitic nematode, is established in North America, forest owners will have to rely solely on silvicultural treatments to reduce the susceptibility of at-risk pine stands to S. noctilio attack. Silvicultural treatments including precommercial thinning, promoting optimal growing conditions for pines on a given site, reducing numbers of susceptible hosts, and consistent monitoring of stands are suggested activities to help protect pine stands from invasion by S. noctilio.


2002 ◽  
Vol 55 ◽  
pp. 433-433
Author(s):  
B.A. Gresham ◽  
M.K. Kay ◽  
W. Faulds ◽  
T.M. Withers

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