In vivo tissue response to resorbable silica xerogels as controlled-release materials

Abstract Background A compact passive oxide layer can grow on tantalum (Ta). It has been reported that this oxide layer can facilitate bone ingrowth in vivo though the development of bone-like apatite, which promotes hard and soft tissue adhesion. Thus, Ta surface treatment on facial implant materials may improve the tissue response, which could result in less fibrotic encapsulation and make the implant more stable on the bone surface. The purposes of this study were to verify whether surface treatment of facial implant materials using Ta can improve the biohistobiological response and to determine the possibility of potential clinical applications. Methods Two different and commonly used implant materials, silicone and expanded polytetrafluoroethylene (ePTFE), were treated via Ta ion implantation using a Ta sputtering gun. Ta-treated samples were compared with untreated samples using in vitro and in vivo evaluations. Osteoblast (MG-63) and fibroblast (NIH3T3) cell viability with the Ta-treated implant material was assessed, and the tissue response was observed by placing the implants over the rat calvarium (n = 48) for two different lengths of time. Foreign body and inflammatory reactions were observed, and soft tissue thickness between the calvarium and the implant as well as the bone response was measured. Results The treatment of facial implant materials using Ta showed a tendency toward increased fibroblast and osteoblast viability, although this result was not statistically significant. During the in vivo study, both Ta-treated and untreated implants showed similar foreign body reactions. However, the Ta-treated implant materials (silicone and ePTFE) showed a tendency toward better histological features: lower soft tissue thickness between the implant and the underlying calvarium as well as an increase in new bone activity. Conclusion Ta surface treatment using ion implantation on silicone and ePTFE facial implant materials showed the possibility of reducing soft tissue intervention between the calvarium and the implant to make the implant more stable on the bone surface. Although no statistically significant improvement was observed, Ta treatment revealed a tendency toward an improved biohistological response of silicone and ePTFE facial implants. Conclusively, tantalum treatment is beneficial and has the potential for clinical applications.

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Real‐Time In Vivo Detection of Cellular Senescence through the Controlled Release of the NIR Fluorescent Dye Nile Blue

Angewandte Chemie International Edition ◽

10.1002/anie.202004142 ◽

2020 ◽

Vol 59 (35) ◽

pp. 15152-15156 ◽

Cited By ~ 2

Author(s):

Beatriz Lozano‐Torres ◽

Juan F. Blandez ◽

Irene Galiana ◽

Alba García‐Fernández ◽

María Alfonso ◽

...

Keyword(s):

Controlled Release ◽

Real Time ◽

Cellular Senescence ◽

Nile Blue ◽

Fluorescent Dye ◽

In Vivo Detection

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Combination of hybrid peptide with biodegradable gelatin hydrogel for controlled release and enhancement of anti-tumor activity in vivo

Journal of Controlled Release ◽

10.1016/j.jconrel.2013.12.021 ◽

2014 ◽

Vol 176 ◽

pp. 1-7 ◽

Cited By ~ 42

Author(s):

Arong Gaowa ◽

Tomohisa Horibe ◽

Masayuki Kohno ◽

Keisuke Sato ◽

Hiroshi Harada ◽

...

Keyword(s):

Controlled Release ◽

Hybrid Peptide ◽

Gelatin Hydrogel ◽

Anti Tumor Activity

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Controlled Release of Endothelial Cell Growth Factor from Chitosan-Albumin Microspheres for Localized Angiogenesis: In Vitro and in Vivo Studies

Artificial Cells Blood Substitutes and Biotechnology ◽

10.3109/10731199609117438 ◽

1996 ◽

Vol 24 (3) ◽

pp. 257-271 ◽

Cited By ~ 46

Author(s):

Y. Murat Elçin ◽

Vivek Dixit ◽

Gary Gitnick

Keyword(s):

Endothelial Cell ◽

Controlled Release ◽

Growth Factor ◽

Cell Growth ◽

In Vivo Studies ◽

Albumin Microspheres ◽

Endothelial Cell Growth Factor ◽

Endothelial Cell Growth

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The Biological Effects of Combining Metals in a Posterior Spinal Implant: In Vivo Model Development Report of the First Two Cases

Advances in Orthopedic Surgery ◽

10.1155/2014/761967 ◽

2014 ◽

Vol 2014 ◽

pp. 1-9 ◽

Cited By ~ 1

Author(s):

Christine L. Farnsworth ◽

Peter O. Newton ◽

Eric Breisch ◽

Michael T. Rohmiller ◽

Jung Ryul Kim ◽

...

Keyword(s):

Galvanic Corrosion ◽

Biological Effects ◽

Model Development ◽

Spinal Instrumentation ◽

Pedicle Screws ◽

Tissue Response ◽

In Vivo Model ◽

Particle Count ◽

Spinal Implant

Study Design. Combinations of metal implants (stainless steel (SS), titanium (Ti), and cobalt chrome (CC)) were placed in porcine spines. After 12 months, tissue response and implant corrosion were compared between mixed and single metal junctions. Objective. Model development and an attempt to determine any detriment of combining different metals in posterior spinal instrumentation. Methods. Yucatan mini-pigs underwent instrumentation over five unfused lumbar levels. A SS rod and a Ti rod were secured with Ti and SS pedicle screws, SS and Ti crosslinks, SS and CC sublaminar wires, and Ti sublaminar cable. The resulting 4 SS/SS, 3 Ti/Ti, and 11 connections between dissimilar metals per animal were studied after 12 months using radiographs, gross observation, and histology (foreign body reaction (FBR), metal particle count, and inflammation analyzed). Results. Two animals had constructs in place for 12 months with no complications. Histology of tissue over SS/SS connections demonstrated 11.1 ± 7.6 FBR cells, 2.1 ± 1.7 metal particles, and moderate to extensive inflammation. Ti/Ti tissue showed 6.3 ± 3.8 FBR cells, 5.2 ± 6.7 particles, and no to extensive inflammation (83% extensive). Tissue over mixed components had 14.1 ± 12.6 FBR cells and 13.4 ± 27.8 particles. Samples surrounding wires/cables versus other combinations demonstrated FBR (12.4 ± 13.5 versus 12.0 ± 9.6 cells, P = 0.96), particles (19.8 ± 32.6 versus 4.3 ± 12.7, P = 0.24), and inflammation (50% versus 75% extensive, P = 0.12). Conclusions. A nonfusion model was developed to study corrosion and analyze biological responses. Although no statistical differences were found in overlying tissue response to single versus mixed metal combinations, galvanic corrosion between differing metals is not ruled out. This pilot study supports further investigation to answer concerns when mixing metals in spinal constructs.

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