In vivo bone regeneration with injectable calcium phosphate biomaterial: A three-dimensional micro-computed tomographic, biomechanical and SEM study

Biomaterials ◽  
2005 ◽  
Vol 26 (27) ◽  
pp. 5444-5453 ◽  
Author(s):  
O GAUTHIER ◽  
R MULLER ◽  
D VONSTECHOW ◽  
B LAMY ◽  
P WEISS ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Bone Regeneration ◽  
Three Dimensional ◽  
Computed Tomographic ◽  
Sem Study

In vitro and in vivo biocompatibility of calcium‐phosphate scaffolds three‐dimensional printed by stereolithography for bone regeneration

2019 ◽  
Vol 108 (3) ◽  
pp. 412-425 ◽  
Author(s):  
Laurent Le Guéhennec ◽  
Dorien Van hede ◽  
Erwan Plougonven ◽  
Grégory Nolens ◽  
Bruno Verlée ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Bone Regeneration ◽  
Three Dimensional

scholarly journals Biphasic Calcium Phosphate Biomaterials: Stem Cell-Derived Osteoinduction or In Vivo Osteoconduction? Novel Insights in Maxillary Sinus Augmentation by Advanced Imaging

Materials ◽  
2021 ◽  
Vol 14 (9) ◽  
pp. 2159
Author(s):  
Giovanna Iezzi ◽  
Antonio Scarano ◽  
Luca Valbonetti ◽  
Serena Mazzoni ◽  
Michele Furlani ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Maxillary Sinus ◽  
Biphasic Calcium Phosphate ◽  
Calcium Phosphates ◽  
Three Dimensional ◽  
Sinus Augmentation ◽  
Native Bone ◽  
Maxillary Sinus Augmentation ◽  
Posterior Maxilla

Maxillary sinus augmentation is often necessary prior to implantology procedure, in particular in cases of atrophic posterior maxilla. In this context, bone substitute biomaterials made of biphasic calcium phosphates, produced by three-dimensional additive manufacturing were shown to be highly biocompatible with an efficient osteoconductivity, especially when combined with cell-based tissue engineering. Thus, in the present research, osteoinduction and osteoconduction properties of biphasic calcium-phosphate constructs made by direct rapid prototyping and engineered with ovine-derived amniotic epithelial cells or amniotic fluid cells were evaluated. More in details, this preclinical study was performed using adult sheep targeted to receive scaffold alone (CTR), oAFSMC, or oAEC engineered constructs. The grafted sinuses were explanted at 90 days and a cross-linked experimental approach based on Synchrotron Radiation microCT and histology analysis was performed on the complete set of samples. The study, performed taking into account the distance from native surrounding bone, demonstrated that no significant differences occurred in bone regeneration between oAEC-, oAFMSC-cultured, and Ctr samples and that there was a predominant action of the osteoconduction versus the stem cells osteo-induction. Indeed, it was proven that the newly formed bone amount and distribution decreased from the side of contact scaffold/native bone toward the bulk of the scaffold itself, with almost constant values of morphometric descriptors in volumes more than 1 mm from the border.

scholarly journals Three-Dimensional Zirconia-Based Scaffolds for Load-Bearing Bone-Regeneration Applications: Prospects and Challenges

Materials ◽  
2021 ◽  
Vol 14 (12) ◽  
pp. 3207
Author(s):  
Kumaresan Sakthiabirami ◽  
Vaiyapuri Soundharrajan ◽  
Jin-Ho Kang ◽  
Yunzhi Peter Yang ◽  
Sang-Won Park
Keyword(s):  
Bone Regeneration ◽  
Biological Activities ◽  
Three Dimensional ◽  
In Vivo Studies ◽  
High Mechanical Strength ◽  
Real World Applications ◽  
3D Fabrication ◽  
Dental Applications

The design of zirconia-based scaffolds using conventional techniques for bone-regeneration applications has been studied extensively. Similar to dental applications, the use of three-dimensional (3D) zirconia-based ceramics for bone tissue engineering (BTE) has recently attracted considerable attention because of their high mechanical strength and biocompatibility. However, techniques to fabricate zirconia-based scaffolds for bone regeneration are in a stage of infancy. Hence, the biological activities of zirconia-based ceramics for bone-regeneration applications have not been fully investigated, in contrast to the well-established calcium phosphate-based ceramics for bone-regeneration applications. This paper outlines recent research developments and challenges concerning numerous three-dimensional (3D) zirconia-based scaffolds and reviews the associated fundamental fabrication techniques, key 3D fabrication developments and practical encounters to identify the optimal 3D fabrication technique for obtaining 3D zirconia-based scaffolds suitable for real-world applications. This review mainly summarized the articles that focused on in vitro and in vivo studies along with the fundamental mechanical characterizations on the 3D zirconia-based scaffolds.

scholarly journals In Vitro and In Vivo Evaluation of a Three-Dimensional Porous Multi-Walled Carbon Nanotube Scaffold for Bone Regeneration

Nanomaterials ◽  
2017 ◽  
Vol 7 (2) ◽  
pp. 46 ◽  
Author(s):  
Manabu Tanaka ◽  
Yoshinori Sato ◽  
Mei Zhang ◽  
Hisao Haniu ◽  
Masanori Okamoto ◽  
...  
Keyword(s):  
Carbon Nanotube ◽  
Bone Regeneration ◽  
Three Dimensional ◽  
In Vivo Evaluation ◽  
Multi Walled Carbon Nanotube

A Novel Animal Model for Three-Dimensional Horizontal and Vertical Bone Regeneration Using Osseous Shell Technique: A Pre-clinical In-Vivo Study in Rabbits

2021 ◽  
Author(s):  
Mohammad Kamal ◽  
Sara Al-Obaidly ◽  
Bernd Lethaus ◽  
Alexander K. Bartella
Keyword(s):  
Animal Model ◽  
Bone Regeneration ◽  
Bone Grafting ◽  
Rabbit Model ◽  
Three Dimensional ◽  
Materials Testing ◽  
Bone Augmentation ◽  
Mandibular Ramus ◽  
Skeletal Defects

Abstract Background: Bone grafting is commonly used for reconstructing skeletal defects in the craniofacial region. Several bone augmentation models were developed to optimize bone regeneration in both vertical and horizontal dimesions. Aim: The aim of this study was to develop a surgical animal model for establishing a three-dimensional (3D) grafting environment in the animal's mandibular ramus for horizontal and vertical bone regeneration using osseous shell technique, as in human patients. Materials and methods: Initial osteological and imaging survey were performed on a postmortem skull of a New Zealand White (NZW) rabbit skull, Oryctolagus cuniculus, for feasibility assessment for performing the surgical procedure. 3D osseus defect was created in the mandibular ramus through a submandibular incision and the osseous shell plates were stabilized with osteosynthesis fixation screws and defect filled with particular bone grafting material. The in-vivo surgical procedures were conducted in four 8-week-old NZW rabbits utilising two osseous shell materials: xenogenic human cortical plates, and autogenous rabbit cortical plates, and the created 3D defects were filled using xenograft and allograft bone grafting materials. The healed defects were evaluated for bone regeneration after 12 weeks using histological and Cone Beam Computed Tomography (CBCT) imaging analysis. Results: Clinical analysis at 12 weeks after surgery revealed the stability of the 3D grafted bone augmentation defects using the osseous shell technique. Imaging and histological analyses confirmed the effectiveness of this model in assessing bone regeneration. Conclusion: The rabbit model is an efficient and reliable biological method for creating a seizable three-dimensional horizontal and vertical bone regeneration model in the mandibular ramus using osseous shell technique for testing various bone-substitute materials testing without compromising the health of the animal. The filled defects could be analyzed for osteogenesis, quantification of bone formation, and healing potential, using histomorphometric analysis, in addition to 3D morphologic evaluation using radiation imaging.

scholarly journals In vivo bone regeneration assessment of offset and gradient melt electrowritten (MEW) PCL scaffolds

2020 ◽  
Vol 24 (1) ◽  
Author(s):  
Naghmeh Abbasi ◽  
Ryan S. B. Lee ◽  
Saso Ivanovski ◽  
Robert M. Love ◽  
Stephen Hamlet
Keyword(s):  
Bone Regeneration ◽  
Size Structure ◽  
Three Dimensional ◽  
Immunohistochemical Analysis ◽  
Repair Process ◽  
Micro Computed Tomography ◽  
Calvarial Defects ◽  
Promising Solution ◽  
Poly Caprolactone

Abstract Background Biomaterial-based bone tissue engineering represents a promising solution to overcome reduced residual bone volume. It has been previously demonstrated that gradient and offset architectures of three-dimensional melt electrowritten poly-caprolactone (PCL) scaffolds could successfully direct osteoblast cells differentiation toward an osteogenic lineage, resulting in mineralization. The aim of this study was therefore to evaluate the in vivo osteoconductive capacity of PCL scaffolds with these different architectures. Methods Five different calcium phosphate (CaP) coated melt electrowritten PCL pore sized scaffolds: 250 μm and 500 μm, 500 μm with 50% fibre offset (offset.50.50), tri layer gradient 250–500-750 μm (grad.250top) and 750–500-250 μm (grad.750top) were implanted into rodent critical-sized calvarial defects. Empty defects were used as a control. After 4 and 8 weeks of healing, the new bone was assessed by micro-computed tomography and immunohistochemistry. Results Significantly more newly formed bone was shown in the grad.250top scaffold 8 weeks post-implantation. Histological investigation also showed that soft tissue was replaced with newly formed bone and fully covered the grad.250top scaffold. While, the bone healing did not happen completely in the 250 μm, offset.50.50 scaffolds and blank calvaria defects following 8 weeks of implantation. Immunohistochemical analysis showed the expression of osteogenic markers was present in all scaffold groups at both time points. The mineralization marker Osteocalcin was detected with the highest intensity in the grad.250top and 500 μm scaffolds. Moreover, the expression of the endothelial markers showed that robust angiogenesis was involved in the repair process. Conclusions These results suggest that the gradient pore size structure provides superior conditions for bone regeneration.

Guided Bone Regeneration of Femoral Segmental Defects using Equine Bone Graft: An In-Vivo Micro-Computed Tomographic Study in Rats

2018 ◽  
Vol 32 (5) ◽  
pp. 456-466 ◽  
Author(s):  
Mohammed Awadh Binsalah ◽  
Sundar Ramalingam ◽  
Mohammed Alkindi ◽  
Nasser Nooh ◽  
Khalid Al-Hezaimi
Keyword(s):  
Bone Graft ◽  
Bone Regeneration ◽  
Guided Bone Regeneration ◽  
Computed Tomographic ◽  
Equine Bone

Impact of Calcium Phosphate Particle Morphology on Osteoconduction: an In Vivo Study

2007 ◽  
Vol 361-363 ◽  
pp. 1237-1240 ◽  
Author(s):  
Christophe Drouet ◽  
Ronan Barré ◽  
Gérard Brunel ◽  
Gérard Dechambre ◽  
Edmond Benqué ◽  
...  
Keyword(s):  
Hydrothermal Synthesis ◽  
Chemical Composition ◽  
Calcium Phosphate ◽  
Bone Regeneration ◽  
Particle Morphology ◽  
In Vivo Study ◽  
Total Absence

Apatite/β−TCP particles exhibiting non-conventional urchin-like morphology were prepared by hydrothermal synthesis. Their implantation in the rat calvarium was followed during 60 days. A total absence of osteoconduction was observed despite a favorable chemical composition, stressing the fundamental role of particle morphology on bone regeneration. Results are discussed in relation with other literature data. Possible explanations include the disfavored accumulation of biological mediators due to the acicular shape of the particles and/or a limited accessibility for cells.

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