Exploring the Binding Mode of Ester-based Cationic Gemini Surfactants with Calf Thymus DNA: A Detailed Physicochemical, Spectroscopic and Theoretical Study

A new tin(IV) corrole, 5,10,15-tris(4-methoxycarbonylphenyl) corrole tin(IV) (1-Sn) was synthesized and characterized. The DNA binding, photocleavage and anti-cancer activity were studied and compared with its free-base. The interaction of 1-Sn and its free-base 1 with calf thymus DNA had been investigated by spectroscopic methods, viscosity measurements and molecular docking analysis. The results revealed that 1-Sn and 1 could interact with calf thymus DNA via an outside groove binding mode. Furthermore, although 1 displayed no photonuclease activity, 1-Sn exhibited good photonuclease activity as indicated by agarose gel electrophoresis, and superoxide anion might be the active intermediate for the DNA scission. Finally, 1 was nontoxic but 1-Sn displayed cytotoxicity towards A549 tumor cell lines.

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Non-covalent interaction between CA–TAT and calf thymus DNA: Deciphering the binding mode by in vitro studies

International Journal of Biological Macromolecules ◽

10.1016/j.ijbiomac.2017.11.158 ◽

2018 ◽

Vol 114 ◽

pp. 1354-1360 ◽

Cited By ~ 9

Author(s):

Mingxiu Lv ◽

Mengwei Wang ◽

Kui Lu ◽

Bingchao Duan ◽

Yufen Zhao

Keyword(s):

Binding Mode ◽

In Vitro Studies ◽

Calf Thymus Dna ◽

Covalent Interaction ◽

Calf Thymus

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Studies of the binding mode of TXNHCH 2 COOH with calf thymus DNA by spectroscopic methods

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy ◽

10.1016/j.saa.2016.06.032 ◽

2016 ◽

Vol 169 ◽

pp. 128-133 ◽

Cited By ~ 7

Author(s):

Nese Ataci ◽

Nergis Arsu

Keyword(s):

Binding Mode ◽

Calf Thymus Dna ◽

Spectroscopic Methods ◽

Calf Thymus

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Synthesis of New Five Coordinated Copper(II) and Nickel(II) Complexes of L-Valine and Kinetic Study of Copper(II) with Calf Thymus DNA

Metal-Based Drugs ◽

10.1155/mbd.2002.81 ◽

2002 ◽

Vol 9 (1-2) ◽

pp. 81-90 ◽

Cited By ~ 3

Author(s):

Aijaz Ahmad Tak ◽

Farukh Arjmand ◽

Sartaj Tabassum

Keyword(s):

Metal Ion ◽

Binding Mode ◽

Molar Conductance ◽

Order Conditions ◽

Calf Thymus Dna ◽

First Order ◽

Reversible Redox ◽

Calf Thymus ◽

Vis Spectroscopy ◽

Kinetics Of

Five coordinated novel complexes of CuII and NiII have been synthesized from benzil and 1,3- diaminopropane-CuII/NiII complex and characterized by elemental analysis, i.r., n.m.r., e.p.r, molar conductance and u.v-vis, spectroscopy. The complexes are ionic in nature and exhibit pentaeoordinated geometry around the metal ion. The reaction kinetics of C25H36N5O2CuCl with calf thymus DNA was studied by u.v-vis, spectroscopy in aqueous medium. The complex after interaction with calf thymus DNA shows shift in the absorption spectrum and hypochromicity indicating an intercalative binding mode. The Kobs values have been calculated under pseudo-first order conditions. The redox behaviour of complex C25H36N5O2CuCl in the presence and in the absence of calf thymus DNA in the aqueous solution has been investigated by cyclic voltammetry. The cyclic voitammogram exhibits one quasi-reversible redox wave corresponding to CuII/CuI redox couple with E1/2 values of -0.377 and -0.237 V respectively at a scan rate of 0.1Vs-1 .On interaction with calf thymus DNA, the complex C25H36N5O2CuCl exhibits shifts in both Ep as well as in E1/2 values, indicating strong binding of the complex to the calf thymus DNA.

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