Effect of cyclosporin A on human gingival fibroblast collagen turnover in relation to the development of gingival overgrowth: an in vitro study

The aim of this study is to determine the cytotoxicity of three different nano composite resins (CRs) on human gingival fibroblast (hGF) and periodontal ligament fibroblast (hPDLF) cell lines. These CRs selected were nanohybrid organic monomer-based Admira Fusion (AF), nanohybrid Bis-(acryloyloxymethyl) tricyclo [5.2.1.0.sup.2,6] decane-based Charisma Topaz (CT), and supra nano filled resin-based Estelite Quick Sigma (EQS). MTT assay was performed to assess the cytotoxicity of CRs at 24 h and one week. AF and EQS applied on hGF cells at 24 h and one week demonstrated similar cytotoxic outcomes. Cytotoxicity of CT on hGF cells at one week was higher than 24 h (p = 0.04). Cytotoxicity of CT on hGF cells was higher at 24 h (p = 0.002) and one week (p = 0.009) compared to control. All composites showed higher cytotoxicity on hPDLF cells at one week than the 24 h (AF; p = 0.02, CT; p = 0.02, EQS; p = 0.04). AF and EQS demonstrated lower cytotoxicity on hPDLF cells than the control group at 24 h (AF; p = 0.01, EQS; p = 0.001). CT was found more cytotoxic on hPDLF cells than the control (p = 0.01) and EQS group (p = 0.008) at one week. The cytotoxicity of CRs on hGF and hPDLF cells vary, according to the type of composites, cell types, and exposure time.

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Effect of Lipid Composition on Topical Delivery of Cyclosporin-A from Nonionic Ldtosomal Formulations: An in Vitro Study with Hairless Mouse Skin

Journal of Liposome Research ◽

10.3109/08982109709035501 ◽

1997 ◽

Vol 7 (2-3) ◽

pp. 261-271 ◽

Cited By ~ 3

Author(s):

Neti Waranuch ◽

Chandrasekharan Ramachandran ◽

Norman D. Weiner

Keyword(s):

Cyclosporin A ◽

Lipid Composition ◽

Mouse Skin ◽

In Vitro Study ◽

Topical Delivery ◽

Hairless Mouse ◽

Vitro Study ◽

Hairless Mouse Skin

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Guided gingival fibroblast attachment to titanium surfaces: an in vitro study

Journal Of Clinical Periodontology ◽

10.1111/jcpe.12025 ◽

2012 ◽

Vol 40 (1) ◽

pp. 99-108 ◽

Cited By ~ 16

Author(s):

Victoria R Kearns ◽

Rachel L. Williams ◽

Felora Mirvakily ◽

Patrick J. Doherty ◽

Nicolas Martin

Keyword(s):

In Vitro Study ◽

Vitro Study ◽

Gingival Fibroblast ◽

Fibroblast Attachment ◽

Titanium Surfaces

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Molecular Aspects of Drug-Induced Gingival Overgrowth: An In Vitro Study on Amlodipine and Gingival Fibroblasts

International Journal of Molecular Sciences ◽

10.3390/ijms20082047 ◽

2019 ◽

Vol 20 (8) ◽

pp. 2047 ◽

Cited By ~ 2

Author(s):

Dorina Lauritano ◽

Alberta Lucchese ◽

Dario Di Stasio ◽

Fedora Della Vella ◽

Francesca Cura ◽

...

Keyword(s):

Gene Expression ◽

Extracellular Matrix ◽

In Vitro Study ◽

Gingival Fibroblast ◽

Gingival Hyperplasia ◽

Drug Induced ◽

Gingival Overgrowth ◽

Molecular Aspects ◽

Gene Expression Levels

Gingival overgrowth is a serious side effect that accompanies the use of amlodipine. Several conflicting theories have been proposed to explain the fibroblast’s function in gingival overgrowth. To determine whether amlodipine alters the fibrotic response, we investigated its effects on treated gingival fibroblast gene expression as compared with untreated cells. Materials and Methods: Fibroblasts from ATCC® Cell Lines were incubated with amlodipine. The gene expression levels of 12 genes belonging to the “Extracellular Matrix and Adhesion Molecules” pathway was investigated in treated fibroblasts cell culture, as compared with untreated cells, by real time PCR. Results: Most of the significant genes were up-regulated. (CTNND2, COL4A1, ITGA2, ITGA7, MMP10, MMP11, MMP12, MMP26) except for COL7A1, LAMB1, MMP8, and MMP16, which were down-regulated. Conclusion: These results seem to demonstrate that amlodipine has an effect on the extracellular matrix of gingival fibroblast. In the future, it would be interesting to understand the possible effect of the drug on fibroblasts of patients with amlodipine-induced gingival hyperplasia.

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