Fuel Ethanol Production from Wet Oxidised Corn Stover by S. Cerevisiae

2011 ◽  
Vol 343-344 ◽  
pp. 963-967 ◽  
Author(s):  
Zhang Qiang ◽  
Anne Belinda Thomsen
Keyword(s):  
Ethanol Production ◽  
Corn Stover ◽  
Simultaneous Saccharification And Fermentation ◽  
Ethanol Concentration ◽  
Ethanol Yield ◽  
Liquid Fraction ◽  
Raw Material ◽  
Wet Oxidation ◽  
Inhibition Effect ◽  
Simultaneous Saccharification

In order to find out appropriate process for ethanol production from corn stover, wet oxidation（195°C，15 minutes）and simultaneous saccharification and fermentation (SSF) was carried out to produce ethanol. The results showed that the cellulose recovery of 92.9% and the hemicellulose recovery of 74.6% were obtained after pretreatment. 86.5% of cellulose was remained in the solid cake . After 24h hydrolysis at 50°C using cellulase（Cellubrix L）,the achieved conversion of cellulose to glucose was 64.8%. Ethanol production was evaluated from dried solid cake and the hydrolysate was employed as liquid fraction . After 142 h of SSF with substrate concentration of 8% (W/V), ethanol yield of 73.1 % of the theoretical based on glucose in the raw material was obtained by S. cerevisiae(ordinary baker’ yeast) . The corresponding ethanol concentration and volumetric productivity were 17.2g/L and 0.121g/L.h respectively. The estimated total ethanol production was 257.7 kg/ton raw material by assuming consumption of both C-6 and C-5. No obvious inhibition effect occurred during SSF. These instructions give you the basic guidelines for preparing papers for WCICA/IEEE conference proceedings.

Extractive Fermentation of Ethanol from Fresh Cassava Roots Using Vacuum Fractionation Technique

2014 ◽  
Vol 931-932 ◽  
pp. 1096-1100 ◽  
Author(s):  
Supathra Phakping ◽  
Mariena Ketudat-Cairns ◽  
Apichat Boontawan
Keyword(s):  
Simultaneous Saccharification And Fermentation ◽  
Ethanol Concentration ◽  
Fermentation Broth ◽  
Product Inhibition ◽  
Raw Material ◽  
Yeast Cells ◽  
Batch Mode ◽  
Inhibition Effect ◽  
Vacuum Fractionation ◽  
Simultaneous Saccharification

Fresh cassava roots were used as a raw material for ethanol fermentation. Conventional batch mode (CF), simultaneous saccharification and fermentation (SSF) and simultaneous liquefaction, saccharification and fermentation (SLSF) were investigated with various enzyme systems. The ethanol concentrations obtained in batch fermentations ranged from 8-12 wt%. In addition, vacuum fractionating technique was successfully introduced to simultaneously remove high purity ethanol from fermentation broth in batch mode. The distilled ethanol concentration was approximately 86 wt% whilst its concentration in the bioreactor was kept lower than 2 wt%. As a result, the product inhibition effect to yeast cells was reduced.

Production of Fuel Ethanol from Hydrotherm-Pretreated Corn Stover by Pichia stipitis

2013 ◽  
Vol 641-642 ◽  
pp. 943-946
Author(s):  
Qiang Zhang
Keyword(s):  
Ethanol Production ◽  
Corn Stover ◽  
Substrate Concentration ◽  
Simultaneous Saccharification And Fermentation ◽  
Pichia Stipitis ◽  
Fuel Ethanol ◽  
Hydrothermal Pretreatment ◽  
Raw Material ◽  
Initial Ph ◽  
Simultaneous Saccharification

Corn stover is an abundant raw material for fuel ethanol production. Finding out the appropriate process for ethanol production will be possible to achieve industrilization. Hydrothermal pretreatment (195°C,15min) and simultaneous saccharification and fermentation (SSF) were adopted to produce ethanol from corn stover. The results showed that 86.5% of cellulose were remained in solid cake and most of hemicellulose were solubilized after pretreatment. The highest ethanol concentrationl of 12.12 g/ L was achieved at initial pH of 5.5，temperature 30°C and 130 rpm shaking rate with substrate concentration of 5%(w/v) by Pichia stipitis58376 after 192 h.The corresponding volumetric productivity were 0.065g/Lh .

The Effect of Gleditsia Saponin on Simultaneous Saccharification and Fermentation of Furfural Residue for Ethanol Production

2011 ◽  
Vol 236-238 ◽  
pp. 108-111 ◽  
Author(s):  
Yong Tang ◽  
Zhao Qing Su ◽  
Dan Qing Zhao ◽  
Xiang Qi ◽  
Jian Xin Jiang
Keyword(s):  
Ethanol Production ◽  
Simultaneous Saccharification And Fermentation ◽  
Ethanol Yield ◽  
Liquid Fraction ◽  
Tween 20 ◽  
Enzyme Loading ◽  
Simple Method ◽  
Positive Effects ◽  
Furfural Residue ◽  
Simultaneous Saccharification

Simultaneous saccharification and fermentation is an attractive process configuration for bioethanol. However, the process cost in SSF is still high. Further reductions in cost are expected with reduction of enzyme addition and the use of waste agricultural or industrial materials as feedstock. Surfactant addition is a simple method to reduce the amount of enzyme loading. In the present work we explore the effect of Gleditsia Saponin, an inexpensive and natural surfactant, on SSF of furfural residue, a main waste of furfural industry in China, for ethanol production. Gleditsia Saponin addition even at low concentration, 0.04 g/l, increased the ethanol yield by 9.9%. Meanwhile, when Gleditsia Saponin addition was 0.16 g/l, the yield was increased by 22.8% and the enzyme activity significantly increased in the liquid fraction at the end of SSF, which would contribute to recover and recycle enzyme. Gleditsia Saponin addition at 1.25 g/l had a better performance in improving SSF efficiency than that of 2.5 g/l Tween-20 addition. The positive effects of Gleditsia Saponin addition on SSF, such as less enzyme loading and shorter residence time could also been observed.

Ethanol Production from Corn Stover Using Soaking Pretreatment

2010 ◽  
Vol 171-172 ◽  
pp. 261-265
Author(s):  
Zhuang Zuo ◽  
Xiu Shan Yang
Keyword(s):  
Ethanol Production ◽  
Corn Stover ◽  
Simultaneous Saccharification And Fermentation ◽  
Pichia Stipitis ◽  
Liquid Ratio ◽  
Mild Conditions ◽  
Whole Process ◽  
Conversion Rates ◽  
Mild Temperature ◽  
Simultaneous Saccharification

Corn stover was pretreated using different soaking conditions at mild temperature. Among the tested conditions, the best was 1% NaOH+8% NH4OH，50°C，48 h, Solid-to-liquid ratio 1:10. The results showed that soaking pretreatment achieved 63.6% delignification, retained the xylan and glucan. After enzymatic hydrolysis, conversion rates of xylan and glucan were 70.9% and 78.5%, respectively. The pretreated filtration re-soaking cause 52.7% xylan and 65.0% glucan conversion. NaOH+NH4OH treatment can be performed under mild conditions, gives a good buffering effect, low carbohydates degradation and extensive removal of lignin. Additionally, simultaneous saccharification and fermentation was conducted with pretreated corn stover to assess the ethanol production. For the whole process, 0.15g ethanol /g corn stover was achieved using Saccharomyces cerevisiae Y5, and 0.19g ethanol /g corn stover when using Pichia stipitis.

Expression of serpins by Clostridium thermocellum and simultaneous saccharification of lignocellulosic biomass to enhance ethanol production

BioResources ◽  
2020 ◽  
Vol 15 (4) ◽  
pp. 8662-8676
Author(s):  
Maria Mushtaq ◽  
Muhammad Javaid Asad ◽  
Muhammad Zeeshan Hyder ◽  
Syed Muhammad Saqlan Naqvi ◽  
Saad Imran Malik ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Wheat Straw ◽  
Corn Stover ◽  
Rice Straw ◽  
Clostridium Thermocellum ◽  
Ethanol Yield ◽  
Biofuel Production ◽  
Yeast Fermentation ◽  
Second Generation Bioethanol ◽  
Simultaneous Saccharification

Utilization of biomass for production of second generation bioethanol was considered as a way to reduce burdens of fossil fuel in Pakistan. The materials wheat straw, rice straw, cotton stalk, corn stover, and peel wastes were used in this experiment. Various parameters, such as acidic and alkali pretreatment, enzymatic hydrolysis by cellulases, and effect of proteases inhibitors on ethanol production, were examined. Fermentation was completed by the yeasts Saccharomyces cerevisiae and Clostridium thermocellum separately, and their ethanol production were compared and maximum ethanol yield was obtained with wheat straw i.e.,11.3 g/L by S. cerevisiae and 8.5 g/L by C. thermocellum. Results indicated that a higher quantity of sugar was obtained from wheat straw (19.6 ± 1.6 g/L) followed by rice straw (17.6 ± 0.6 g/L) and corn stover (16.1 ± 0.9 g/L) compared to the other evaluated biomass samples. A higher yield of ethanol (11.3 g/L) was observed when a glucose concentration of 21.7 g/L was used, for which yeast fermentation efficiency was 92%. Results also revealed the increased in ethanol production (93%) by using celluases in combination with recombinant Serine protease inhibitors from C. thermocellum. It is expected that the use of recombinant serpins with cellulases will play a major role in the biofuel production by using agricultural biomass. This will also help in the economics of the biofuel.

scholarly journals Effect of Pre-hydrolysis on Simultaneous Saccharification and Fermentation of Native Rye Starch

2020 ◽  
Vol 13 (6) ◽  
pp. 923-936 ◽  
Author(s):  
Ewelina Strąk-Graczyk ◽  
Maria Balcerek
Keyword(s):  
Dry Matter ◽  
Agricultural Sector ◽  
Simultaneous Saccharification And Fermentation ◽  
Ethanol Yield ◽  
Matter Content ◽  
Raw Material ◽  
Process Efficiency ◽  
Dry Matter Content ◽  
Sem Images ◽  
Simultaneous Saccharification

Abstract The rising population and increasing demand for food place added pressure on the agricultural sector to maintain high process efficiency while implementing environmentally friendly methods. In this study, we investigate the effect of pre-hydrolysis of native rye starch and its influence on the yield of ethanol obtained by simultaneous saccharification and fermentation (SSF) from high gravity rye mashes with 25% and 28% w w−1 dry matter content. Fermentation was carried out in a 3-day system at a temperature of 35 ± 1 °C using the dry distillery yeast Ethanol Red (Saccharomyces cerevisiae). The characteristics of the tested raw material and changes in the native rye starch during enzymatic hydrolysis were analyzed using a scanning electron microscope (SEM). The SEM images revealed characteristic changes on the surface of the starch, which was found to have a layered structure, as well as interesting behavior by the yeast during SSF when the glucose concentration in the environment was lowered. Both in the mashes with 25% and 28% w w−1 dry matter, starch pre-hydrolysis did not significantly increase either the initial amounts of sugars available to the yeast or the fermentation efficiency and ethanol yield in comparison to the mashes without this pre-treatment.

Proper ultrasound treatment increases ethanol production from simultaneous saccharification and fermentation of sugarcane bagasse

RSC Advances ◽  
2016 ◽  
Vol 6 (94) ◽  
pp. 91409-91419 ◽  
Author(s):  
Rajendran Velmurugan ◽  
Aran Incharoensakdi
Keyword(s):  
Ethanol Production ◽  
Sugarcane Bagasse ◽  
Simultaneous Saccharification And Fermentation ◽  
Ethanol Yield ◽  
Ultrasound Treatment ◽  
Fermentation Processes ◽  
Cellulase Complex ◽  
Simultaneous Saccharification

To improve the saccharification and fermentation processes, proper ultrasound was applied which resulted in the presence of cellulase complex with improved β-glucosidase ratio leading to enhanced overall ethanol yield.

Optimization of Simultaneous Saccharification and Fermentation for Ethanol Production from Steam-Exploded Cotton Stalk

2013 ◽  
Vol 724-725 ◽  
pp. 391-398
Author(s):  
Qin Zhang ◽  
Yan Bin Li ◽  
Zhan Wen Liu ◽  
Yun Feng Pu ◽  
Li Ming Xia
Keyword(s):  
Ethanol Production ◽  
Simultaneous Saccharification And Fermentation ◽  
Ph Value ◽  
Raw Material ◽  
Cotton Stalk ◽  
Fermentation Time ◽  
Box Behnken Design ◽  
Initial Ph ◽  
Experimental Yield ◽  
Simultaneous Saccharification

Steam-exploded cotton stalk was used as raw material in ethanol production through simultaneous saccharification and fermentation by Penicillium Q59 and Saccharomyces cerevisiae P1. The fermentative conditions were firstly examined by single factor experiments to determine the central point in Box-Behnken design, which was explored with expectation to get optimized fermentative conditions for enhancement of ethanol production. The results of optimized fermentative conditions were determined as follows: fermentation time was 10.5 days, bran added percent was 15%, initial pH value was 5.5. Under the optimal conditions, the experimental yield of ethanol was 99.85 ± 4.21 g·kg-1SECS (steam-exploded cotton stalk), which was close to the theoretical predicting value, it showed the model was feasible. The research results will provide technical reference for further exploitation of cotton stalk.

scholarly journals Pretreatment of Corn Stover Using an Extremely Low-Liquid Ammonia (ELLA) Method for the Effective Utilization of Sugars in Simultaneous Saccharification and Fermentation (SSF) of Ethanol

Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 191
Author(s):  
Tin Diep Trung Le ◽  
Vi Phuong Nguyen Truong ◽  
My Thi Tra Ngo ◽  
Tae Hyun Kim ◽  
Kyeong Keun Oh
Keyword(s):  
Corn Stover ◽  
Liquid Ammonia ◽  
Elevated Temperatures ◽  
Simultaneous Saccharification And Fermentation ◽  
Aqueous Ammonia ◽  
Ethanol Yield ◽  
Enzymatic Saccharification ◽  
Extended Period ◽  
Pretreatment Conditions ◽  
Simultaneous Saccharification

Extremely low-liquid ammonia (ELLA) pretreatment using aqueous ammonia was investigated in order to enhance the enzymatic saccharification of corn stover and subsequent ethanol production. In this study, corn stover was treated with an aqueous ammonia solution at different ammonia loading rates (0.1, 0.2, and 0.3 g NH3/g biomass) and various liquid-to-solid (L/S) ratios (0.55, 1.12, and 2.5). The ELLA pretreatment was conducted at elevated temperatures (90–150 °C) for an extended period (24–120 h). Thereafter, the pretreated material was saccharified by enzyme digestion and subjected to simultaneous saccharification and fermentation (SSF) tests. The effects of key parameters on both glucan digestibility and xylan digestibility were analyzed using analysis of variance (ANOVA). Under optimal pretreatment conditions (L/S = 2.5, 0.1 g-NH3/g-biomass, 150 °C), 81.2% glucan digestibility and 61.1% xylan digestibility were achieved. The highest ethanol yield achieved on the SSF tests was 85.4%. The ethanol concentration was 14.5 g/L at 96 h (pretreatment conditions: liquid-to-solid ratio (L/S) = 2.5, 0.1 g-NH3/g-biomass, 150 °C, 24 h. SSF conditions: microorganism Saccharomyces cerevisiae (D5A), 15 FPU/g-glucan, CTech2, 3% w/v glucan, 37 °C, 150 rpm).

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