Quantification of the biocontrol agent Trichoderma harzianum with real-time TaqMan PCR and its potential extrapolation to the hyphal biomass

2010 ◽  
Vol 101 (8) ◽  
pp. 2888-2891 ◽  
Author(s):  
Rubén López-Mondéjar ◽  
Anabel Antón ◽  
Stefan Raidl ◽  
Margarita Ros ◽  
José Antonio Pascual
Keyword(s):  
Real Time ◽  
Trichoderma Harzianum ◽  
Biocontrol Agent ◽  
Taqman Pcr

scholarly journals Development of a Real-Time TaqMan PCR Method for Absolute Quantification of the Biocontrol Agent Esteya vermicola

Plant Disease ◽  
2020 ◽  
Vol 104 (6) ◽  
pp. 1694-1700
Author(s):  
Hai-Hua Wang ◽  
Can Yin ◽  
Jie Gao ◽  
Ran Tao ◽  
Chun-Yan Wang ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Biocontrol Agent ◽  
Internal Standard ◽  
Absolute Quantification ◽  
Pinewood Nematode ◽  
Hyphal Length ◽  
Taqman Pcr ◽  
Esteya Vermicola ◽  
The Absolute

Esteya vermicola has been used as an effective biocontrol agent for the management of the pinewood nematode, Bursaphelenchus xylophilus. Tools for monitoring the colonization and parasitism patterns of E. vermicola are required for the development of highly effective biocontrol strategies. Because the TaqMan PCR technique is effective for quantification of species in environmental samples, a real-time PCR-based methodology was developed for absolute quantification of E. vermicola via internal standard addition and extrapolation of DNA quantity to hyphal length. Primers and a probe for the 28S ribosomal RNA gene of E. vermicola were designed, and nested TaqMan real-time PCR-based quantification was performed. In addition, internal standard-based yield measurement was correlated to the absolute quantity of target genomic DNA. Moreover, an extrapolation curve obtained by optical microscopy and image analysis of the mycelia was constructed for the measurement of fungal hyphal length. The absolute quantification method developed in the present study provides a sensitive and accurate technique to quantify fungal density in either wood or other substrate samples and can be used as an effective tool for future studies of biocontrol agents.

scholarly journals Draft Whole-Genome Sequence of the Biocontrol Agent Trichoderma harzianum T6776

2015 ◽  
Vol 3 (3) ◽  
Author(s):  
Riccardo Baroncelli ◽  
Giulia Piaggeschi ◽  
Lisa Fiorini ◽  
Edoardo Bertolini ◽  
Antonio Zapparata ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Trichoderma Harzianum ◽  
Biocontrol Agent ◽  
Whole Genome Sequence ◽  
Whole Genome

Assessment of NS1 gene-specific real time quantitative TaqMan PCR for the detection of Human Bocavirus in respiratory samples

2017 ◽  
Vol 34 ◽  
pp. 53-55 ◽  
Author(s):  
Marco Ligozzi ◽  
Erica Diani ◽  
Federica Lissandrini ◽  
Rosanna Mainardi ◽  
Davide Gibellini
Keyword(s):  
Real Time ◽  
Human Bocavirus ◽  
Taqman Pcr ◽  
Ns1 Gene

Utility of real-time Taqman PCR for antemortem and postmortem diagnosis of human rabies

2013 ◽  
Vol 86 (10) ◽  
pp. 1804-1812 ◽  
Author(s):  
Reeta Subramaniam Mani ◽  
Shampur Narayan Madhusudana ◽  
Anita Mahadevan ◽  
Vijayalakshmi Reddy ◽  
Ashwin Yajaman Belludi ◽  
...  
Keyword(s):  
Real Time ◽  
Human Rabies ◽  
Postmortem Diagnosis ◽  
Taqman Pcr

Differentiation of herpes simplex virus types 1 and 2 in clinical samples by a real-time taqman PCR assay

2005 ◽  
Vol 76 (3) ◽  
pp. 350-355 ◽  
Author(s):  
Lawrence Corey ◽  
Meei-Li Huang ◽  
Stacy Selke ◽  
Anna Wald
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Real Time ◽  
Clinical Samples ◽  
Pcr Assay ◽  
Taqman Pcr ◽  
Simplex Virus

scholarly journals Real-time TaqMan PCR for rapid detection and typing of genes encoding CTX-M extended-spectrum β-lactamases

2007 ◽  
Vol 56 (1) ◽  
pp. 52-55 ◽  
Author(s):  
Christopher I. Birkett ◽  
Hugo A. Ludlam ◽  
Neil Woodford ◽  
Derek F. J. Brown ◽  
Nicholas M. Brown ◽  
...  
Keyword(s):  
Real Time ◽  
Taqman Assay ◽  
Esbl Production ◽  
Pcr Assay ◽  
Extended Spectrum ◽  
Genes Encoding ◽  
Taqman Pcr ◽  
Assay Time ◽  
Phenotypic Methods ◽  
Single Reaction

The prevalence of CTX-M-producing members of the Enterobacteriaceae is increasing worldwide. A novel, multiplex, real-time TaqMan PCR assay to detect and type bla CTX-M genes is described which is an improvement on previously described techniques with respect to reduced assay time, elimination of the need for protracted post-PCR processing and the convenience of a single reaction vessel. Based on β-lactam antibiogram and MIC data, 478 of 1279 Enterobacteriaceae isolates from clinical blood and urine culture specimens were selected and tested for extended-spectrum β-lactamase (ESBL) production using phenotypic methods. The new TaqMan assay detected and typed bla CTX-M genes in 21 of 28 ESBL-producing isolates.

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