scholarly journals Real-time TaqMan PCR for rapid detection of genes encoding five types of non-metallo- (class A and D) carbapenemases in Enterobacteriaceae

2011 ◽  
Vol 38 (1) ◽  
pp. 35-38 ◽  
Author(s):  
R.L. Swayne ◽  
H.A. Ludlam ◽  
V.G. Shet ◽  
N. Woodford ◽  
M.D. Curran
Keyword(s):  
Real Time ◽  
Rapid Detection ◽  
Class A ◽  
Genes Encoding ◽  
Taqman Pcr

Real-Time TaqMan PCR for Rapid Detection and Typing of Prevalent Molecular Class A and D Carbapenemase Genes in Enterobacteriaceae

2011 ◽  
Vol 63 (6) ◽  
pp. e23
Author(s):  
Rosemary Swayne ◽  
Vrinda Shet ◽  
Hugo Ludlam ◽  
Neil Woodford ◽  
Martin Curran
Keyword(s):  
Real Time ◽  
Rapid Detection ◽  
Class A ◽  
Taqman Pcr

scholarly journals Real-time TaqMan PCR for rapid detection and typing of genes encoding CTX-M extended-spectrum β-lactamases

2007 ◽  
Vol 56 (1) ◽  
pp. 52-55 ◽  
Author(s):  
Christopher I. Birkett ◽  
Hugo A. Ludlam ◽  
Neil Woodford ◽  
Derek F. J. Brown ◽  
Nicholas M. Brown ◽  
...  
Keyword(s):  
Real Time ◽  
Taqman Assay ◽  
Esbl Production ◽  
Pcr Assay ◽  
Extended Spectrum ◽  
Genes Encoding ◽  
Taqman Pcr ◽  
Assay Time ◽  
Phenotypic Methods ◽  
Single Reaction

The prevalence of CTX-M-producing members of the Enterobacteriaceae is increasing worldwide. A novel, multiplex, real-time TaqMan PCR assay to detect and type bla CTX-M genes is described which is an improvement on previously described techniques with respect to reduced assay time, elimination of the need for protracted post-PCR processing and the convenience of a single reaction vessel. Based on β-lactam antibiogram and MIC data, 478 of 1279 Enterobacteriaceae isolates from clinical blood and urine culture specimens were selected and tested for extended-spectrum β-lactamase (ESBL) production using phenotypic methods. The new TaqMan assay detected and typed bla CTX-M genes in 21 of 28 ESBL-producing isolates.

Real-Time TaqMan PCR for Rapid Detection and Quantification of Coliforms in Chilled Meat

2015 ◽  
Vol 9 (4) ◽  
pp. 813-822 ◽  
Author(s):  
Shuangfang Hu ◽  
Yigang Yu ◽  
Rong Li ◽  
Xingzhou Xia ◽  
Xinglong Xiao ◽  
...  
Keyword(s):  
Real Time ◽  
Rapid Detection ◽  
Taqman Pcr ◽  
Detection And Quantification

scholarly journals Multiplex PCR for Rapid Detection of Genes Multiplex PCR for Rapid Detection of Genes Encoding Class A Carbapenemases

2012 ◽  
Vol 32 (5) ◽  
pp. 359-361 ◽  
Author(s):  
Sang Sook Hong ◽  
Kyeongmi Kim ◽  
Ji Young Huh ◽  
Bochan Jung ◽  
Myung Seo Kang ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Rapid Detection ◽  
Class A ◽  
Genes Encoding

Rapid detection of Chlamydia/Chlamydophila group in samples collected from swine herds with and without reproductive disorders

2014 ◽  
Vol 17 (2) ◽  
pp. 367-369 ◽  
Author(s):  
K. Rypula ◽  
A. Kumala ◽  
P. Lis ◽  
K. Niemczuk ◽  
K. Płoneczka-Janeczko ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Reproductive Disorders ◽  
Serum Samples ◽  
Swine Herds

Abstract The study was carried out in seven reproductive herds of pigs. In three of them reproductive disorders were observed. Three herds consisted of 10-50 and four consisted of 120-500 adult sows and they were called small and medium, respectively. Fifty-seven adult sows were randomly selected from herds. Serum samples were tested using the complement fixation test and swabs from both eyes and from the vaginal vestibule were examined using real-time PCR. All serum samples were negative. Infected sows were present in each of the study herds. In total, there were 28 positive samples (53%, 28/48) in real-time PCR in sows with reproductive disorders and 35 (53%, 35/66) in sows selected from herds without problems in reproduction. One isolate proved to be Chlamydophila pecorum, whereas all the remaining were Chamydia suis

Rapid detection of OXA-23-like, OXA-24-like, and OXA-58-like carbapenemases from Acinetobacter species by real-time PCR

2020 ◽  
Vol 105 (4) ◽  
pp. 741-746
Author(s):  
M. Mentasti ◽  
K. Prime ◽  
K. Sands ◽  
S. Khan ◽  
M. Wootton
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Acinetobacter Species
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